The PacBio Revio is our new long-read sequencer and replaces our PacBio Sequel IIe
Uses
- De novo whole genome sequencing
- Amplicon sequencing
- Target enrichment
- Iso-seq (RNA sequencing, Methylation detection, Fiber-seq)
Specifications
- The Revio can run up to 4 SMRT cells at a time
- Each SMRTcell can produce up to ~120 Gb of data
- High Fidelity (HiFi) reads with >99% accuracy from templates of 1 – 20 kb in length can be generated from circular consensus sequencing
- Multiplexing available for Iso-seq, amplicon, and bacterial genome sequencing
- Each sample requires only 500 ng input
Details
This instrument is operated by Genomics Core Facility staff in conjunction with next-generation sequencing services.