The CSL Behring Fermentation Facility is a biotechnology pilot plant capable of research- and pilot-scale production of microbial cells, recombinant proteins, and other microbial products over a wide range of controlled conditions. The facility provides equipment and expertise to university-affiliated and independent government and industry researchers interested in fermentation and related technologies, including cell separation and disruption, biomolecule production and purification, and process monitoring.
Upstream Capabilities
- Two Innova 44 shaker incubators - one with refrigeration capability
- Suite of Applikon 500mL mini bioreactors
- Sartorius Biostat B control units running 4 x 5L bioreactors
- Sartorius stainless steel vessels ranging from 30L to 500L with full instrumentation
- Sartorius MFCS supervisory control and data acquisition software with automation facilities
Biomass Processing Capabilities
- Cell lysis through Microfluids LM-20-30 and M-110-EH systems
- Culture broth separation via benchtop and floor centrifuges, Sharples T-1-P and AS-16 tube bowl centrifuges, and GEA Westfalia disk stack centrifuge
- Labconoco FreeZone 18L freeze dryer with bulk tray dryer
- Buchi rotavapor R-100
- Buchi S300 Mini-spray dryer
- YSI 2950 Bioanalyzer
Purification and Analytical Capabilities
- Tangential flow filtration through Sartorius SartoFlow Smart and Advanced units. Typically utilized for dialysis/diafiltration and concentration of proteins
- Fast protein liquid chromatography via GE AKTA Pure 150M
- Thermo Vanquish binary UHPLC system with UV-Vis Variable Wavelength and Refractive Index (RI) detectors
