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The Illumina NextSeq 2000 is our newest short-read sequencer, and has replaced the NextSeq 550.
![Illumina NextSeq](https://www.huck.psu.edu/assets/uploads/content-images/_1024xAUTO_fit_center-center_80_none/insert_illumina_nextseq.jpg)
Uses
The sequencing chemistry is similar across all Illumina platforms. Libraries compatible with one Illumina sequencer should be compatible with all Illumina sequencers.
Used for a variety of applications, including but not limited to:
- De novo whole genome sequencing
- Whole genome resequencing
- Exome sequencing
- RNA-Seq
- scRNA-Seq
- miRNA/small RNA-Seq
- ChIP-Seq
- RIP-Seq
- Degradome sequencing
Specifications
- Three output sizes available: P1, P2, and P3 producing 100 million, 400 million, or 1 billion reads (or read pairs) per run respectively
- P1 is available only as a 300 cycle kit
- P2 and P3 are available as 100, 200 or 300 cycle kits
- One library pool per flow cell, no individual lane loading
- Sample multiplexing available from 96-384 samples, depending on the application
Data produced in the FASTQ format.
This instrument is operated by Genomics Core Facility staff in conjunction with next-generation sequencing services.