Illumina NextSeq 2000

Illumina NextSeq

The Illumina NextSeq 2000 is our newest short-read sequencer, and has replaced the NextSeq 550.   

Illumina NextSeq


  • Three output sizes available: P1, P2, and P3 producing 100 million, 400 million, or 1 billion reads (or read pairs) per run respectively.
  • P1 is available only as a 300 cycle kit.
  • P2 and P3 are available as 100, 200 or 300 cycle kits.
  • One library pool per flow cell, no individual lane loading. 
  • Sample multiplexing available from 96-384 samples, depending on the application.


The sequencing chemistry is similar across all Illumina platforms. Libraries compatible with one Illumina sequencer should be compatible with all Illumina sequencers.

Used for a variety of applications, including but not limited to:

  • De novo whole genome sequencing
  • Whole genome resequencing
  • Exome sequencing
  • RNA-Seq
  • scRNA-Seq
  • miRNA/small RNA-Seq
  • ChIP-Seq
  • RIP-Seq
  • Degradome sequencing

Data produced in the FASTQ format.

This instrument is operated by Genomics Core Facility staff in conjunction with next-generation sequencing services