Microscopy Facility

Nikon N-SIM/N-STORM

Nikon N-STORM

The Nikon N-SIM/N-STORM microscope consolidates two super-resolution techniques on one microscope stand.

N-SIM (structured illumination microscopy)

  • Double the R\resolution of conventional optical microscopes (xy 115 nm, z 300 nm)
  • Applicable to any sample prepared for conventional fluorescence microscopy – no special fluorophore needed
  • Fast temporal resolution of approximately 1 sec/frame
  • Specifications
    • Motorized inverted Nikon ECLIPSE Ti-E TIRF microscope with perfect focus system
    • Lasers: 405 nm, 488 nm, 561 nm, 640 nm
    • Objective lens
    • CFI SR Apochromat TIRF 100×oil (NA1.49)
    • CFI 20x
    • ORCA-Flash 4.0 sCMOS camera
    • NIS-Elements Ar

For more information visit https://www.nikoninstruments.com/Products/Super-resolution/N-SIM-E-Super-Resolution

N-STORM (STochastic Optical Reconstruction Microscopy)

  • The N-STORM super-resolution microscope provides 10 times that of conventional optical microscopes
  • Lateral (XY) resolution ~20-30 nm
  • Axial (Z) resolution ~50 nm

For more information visit https://www.nikoninstruments.com/Products/Super-resolution/N-STORM-Super-Resolution