Research Applications

Research Applications

The Microscopy Core Facility provides microscopic imaging services to the Penn State research community 24 hours per day, 7 days per week. Services include technical support, consultation for instrumentat selection, project planning, training, collaboration, and hands-on experimentation, etc. Some recent publications include:


Banerjee, S., D. Aponte-Diaz, C. Yeager, S.D. Sharma, G. Ning, H.S. Oh, Q. Han, M. Umeda, Y. Hara, R.Y.L. Wang, and C.E. Cameron. 2018. Hijacking of multiple phospholipid biosynthetic pathways and induction of membrane biogenesis by a picornaviral 3CD protein. PLoS Pathog. 14(5):e1007086. doi:10.1371/journal.ppat.1007086.

Oh, H.S., S. Banerjee, D. Aponte-Diaz, S.D. Sharma, J. Aligo, M.F. Lodeiro, G. Ning, R. Sharma, Arnold, J.J., and C.E. Cameron. 2018. Multiple poliovirus-induced organelles suggested by comparison of spatiotemporal dynamics of
membranous structures and phosphoinositides. PLoS Pathog. 14(4):e1007036. doi: 10.1371/journal.ppat.1007036.

Shrestha, S., X. Li, G. Ning, J. Miao, and L. Cui. 2016. The RNA-binding protein Puf1 functions in the maintenance of gametocytes in Plasmodium falciparum. J Cell Sci. 129(16):3144-52. doi:10.1242/jcs.186908.

Law, M., Y. Lee, J.L. Morales, G. Ning, W. Huang, J. Pabon, A.K. Kannan, A.R. Jeong, A. Wood, C. Carter, S. Mohinta, J. Song, and A. August. 2015. Cutting Edge: Drebrin-regulated actin dynamics regulate IgE-dependent mast cell activation and allergic responses. J. Immunol. 195(2):426-30. doi:10.4049/jimmunol.1401442. 

Ye, X., H. Zeng, G. Ning, J.F. Reiter, A. Liu. 2014. C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals. Proc. Natl. Acad. Sci. USA. 111(6):2164-9. doi:10.1073/pnas.1318737111. 

Immunogold Electron Microscopy

Liu, W.S., Y. Zhao, C. Lu, G. Ning, Y. Ma, F. Diaz, and M.A. O'Connor. 2017. Novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle. Reproduction. 153(6):847-863. doi: 10.1530/REP-17-0013. 

Wu, X., Z. Wu, G. Ning, Y. Guo, R. Ali, R.L. Macdonald, A.L. De Blas, B. Luscher, and G. Chen. 2012. γ-Aminobutyric acid type A (GABAA) receptor α subunits play a direct role in synaptic versus extrasynaptic targeting. J. Biol. Chem. 287(33):27417-30. doi: 10.1074/jbc.M112.360461. 

Scanning Electron Microscopy (SEM)

Zheng, Y., D.J. Cosgrove, and G. Ning. 2017. High-resolution field emission scanning electron microscopy (FESEM) imaging of cellulose microfibril organization in plant primary cell walls. Microsc. Microanal. 23(5):1048-1054. doi:

Zheng, Y., X. Wang, Y. Chen, E. Wagner, D.J. 2018. Cosgrove. Xyloglucan in the primary cell wall: Assessment by FESEM, selective enzyme digestions and nanogold
affinity tags. Plant J. 93(2):211-226. doi: 10.1111/tpj.13778. 

Barnes, W.J. and C.T. Anderson. 2018. Cytosolic invertases contribute to cellulose biosynthesis and influence carbon partitioning in seedlings of Arabidopsis thaliana. Plant J. 94(6):956-974. doi: 10.1111/tpj.13909. 

Serial Block-Face Imaging and 3D Ultrastructure

Fredericksen, M.A., Y. Zhang, M.L. Hazen, R.G. Loreto, C.A. Mangold, D.Z. Chen, and D.P. Hughes. 2017. Three-dimensional visualization and a deep-learning model reveal complex fungal parasite networks in behaviorally manipulated ants. Proc. Natl. Acad. Sci. USA. 114(47):12590-12595. doi: 10.1073/pnas.1711673114. 

Wham, D.C., G. Ning, T.C. LaJeunesse. 2017. Symbiodinium glynnii sp. nov., a species of stress-tolerant symbiotic dinoflagellates from pocilloporid and montiporid corals in the Pacific Ocean. Phycologia. 56(4):396-409.

Light Microscopy (Confocal, WF, BF)

Hao, S., L. Ha, G. Cheng, Y. Wan, Y. Xia, D.M. Sosnoski, A.M. Mastro, and S.Y. Zheng. 2018. A spontaneous 3D bone-on-a-chip for bone metastasis study of breast cancer cells. Small. 14(12):e1702787. doi: 10.1002/smll.201702787. 

Ziyuan, G., L. Zhang, W. Zheng, Y. Chen, F. Wang, and G. Chen. 2014. In vivo direct reprogramming of reactive glial cells into functional neurons after brain injury and in an Alzheimer’s disease model. Cell Stem Cell. 14:188-202.