Analytical ultracentrifugation (AUC) is the most versatile, rigorous and accurate means for determining the hydrodynamic shape, size, molar mass and binding thermodynamics of any macromolecule and its complexes.
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recent development made possible by new optical systems. MW-AUC extends the basic hydrodynamic information content of AUC and provides access to a wide range of new applications for biopolymer characterization, and is poised to become an essential analytical tool to study macromolecular interactions.
Uses
Parameters measured from a single SV-AUC sample
- Sedimentation coefficient (for each macromolecule present)
- Diffusion coefficient (averaged for the entire solution; can be derived with 2D analysis)
- Frictional coefficient (averaged for the entire solution; can be derived with 2D analysis)
- Molar Mass estimates for each species
Parameters that can be obtained through multiple SV-AUC cells (spun simultaneously)
- Oligomerization propensity of each macromolecule, through a concentration series
- Demonstration of an interaction between macromolecules, through a concentration series of the two species. This can also be used to determine binding kinetics including Kd values and thermodynamics of the interaction
Specifications
| Fastest Data Acquisition Rate | ABS: <7sec/sector INT: <5sec/scan |
| Max # of Wavelengths | 20 |
| Wavelength Precision | ± 0.5nm |
| Lowest Radial Resolution | 10µm |
| Absorbance Flash Lamp Frequency | 300 Hz |
| CCD Camera Specifications | 2048 x 1088 pixels |
| Interference Fringes | 10 fringes/cell |
| Usable Concentration Ranges | ABS: .005-2mg/mL INT: .025-5mg/mL BSA |
| Operating System | LINUX remote GUI |
| Sample Temperature range | 0-40°C |