Process
- 15 microliters of macromolecular sample is needed for DLS to determine hydrodynamic Radius and Molecular weight. Ideal concentration = 1-2 mg/mL
- Sample is loaded into quartz cuvette (provided). Laser excites the sample and using Brownian motion a series of light scattering measurements are collected at three angles in a matter of minutes and the data averaged
- A Mass model and distribution type is selected. A graph will display bar graph which includes particle size distributions including percentage Rh(nm), %RSD, MW(kD)
- User training is provided for data collection and analysis
Instrumentation
Viscotek 802 Dynamic Light-Scattering Instrument
A state-of-the-art dynamic light-scattering instrument matched with powerful but user-friendly OmniSIZE software to give the most sensitive and accurate hydrodynamic radius.