The DynaPro NanoStar can be operated using microcuvette-based format to analyze size distributions and particle concentrations of proteins, nanomedicines, viruses, vesicles, emulsions, micelles ,and metallic nanoparticles.
Uses
- Hydrodynamic Radius (Rh)
- Intensity, Mass and Number distributions
- Aggregation
- Molecular Weight
- A2 – Second Virial Coefficient
- Temperature transition (Tonset)
Specifications
- Size Range, Rh, by DLS : 0.2 to 25000 nm
- Molar Mass Range by SLS: 300 Da to 1 MDa
- Sensitivity (lysozyme 14.4 kDa): 0.1 mg/mL
- Minimum Sample Volume: 2 µL (quartz cuvette),4 µL (disposable cuvette)
- Laser Wavelength: 658 nm
- Laser Power Control: Programmable 10% to 100%
- Temperature Control: -15 to +120 °C
Details
Traditional batch dynamic light scattering: The DynaPro NanoStar uniquely combines two optimized 90° detections channels: one for DLS and one for static light scattering for determining hydrodynamic Radius (Rh) and Molecular weight. The NanoStar performs robust, accurate measurements with as little as 2 µL of sample.
On-Line with SEC-MALS Dynamic Light Scattering: Using DLS on-line with SEC-MALS will provide hydrodynamic radii and, in combination with MALS, the shape factor Rg:Rh of macromolecules > 10nm.
Acknowledgement
Research reported here is supported by the NIH grant S10 OD030490 for the Wyatt SEC-MALS system to Dr. Neela Yennawar, X-ray Crystallography core facility, Penn State Huck Institutes of the Life Sciences.