Isothermal titration calorimetry (ITC) is the gold standard analytical techniques for in-depth characterization of molecular binding events.
Uses
• Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules
• Binding affinity (Kd), Enthalpy (ΔH), Entropy (ΔS), Stoichiometry (n)
• Dimer dissociation
• Enzyme kinetics
• Assessment of molecular structure changes on binding mechanisms
Specifications
• Operating temperature: 2°C to 80°C
• Autosampler storage temperature: 4°C to ambient
• Response time: 3.3 seconds
• Cell design: 185uL active volume cylindrical cell
• Injection syringe: up to 270uL
• Usable syringe volume: 100µl
• Smallest injection size: 0.1µl
• Stirring speed: up to 200 rpm
• Full Automation 24K Gold cell
Details
The affinity ITC is fully automated, Users can set up samples in 96 well plate in a controlled temperature autosampler. Automated cleaning can also be programmed to reduce contamination between runs. Optional manual mode is available for using even less volume. Affinity ITC is located in a glove box in order to run oxygen sensitive samples.
Acknowledgement
Contributions made by data from this instrument may be acknowledged in papers with language similar to the example below:
We would like to thank Ms. Julia Fecko and Dr. Neela Yennawar for the helpful discussions for the ITC binding study. The work was supported by the NIH grant S10OD025145 to Dr. Yennawar for the TA Instruments Low Volume AutoAffinity ITC, housed in the Automated Biological Calorimetry core facility at Penn State's Huck Institutes of the Life Sciences.