Scott Showalter

Professor of Chemistry; Professor of Biochemistry and Molecular Biology

Scott Showalter

Research Summary

Biophysical Chemistry applied to solution NMR spectroscopy of partially disordered proteins. NMR studies of protein dynamics coupled with computational and theoretical studies of the coupling between nuclear spin relaxation and molecular motion.

Huck Affiliations

Links

Publication Tags

Proteins Protein Intrinsically Disordered Proteins Nuclear Magnetic Resonance Phosphorylation Rna Rna Polymerase Ii Nmr Spectroscopy Magnetic Resonance Spectroscopy Statistical Ensemble Proline Polymers Carbon Application Micrornas Experiments Transcription Factors Nuclear Magnetic Resonance Spectroscopy Epitopes Double Stranded Rna Viral Proteins Masks Rna Polymerase Iii Cyclic Amp Dependent Protein Kinases Lanthanoid Series Elements

Most Recent Publications

Biophysical insights into glucose-dependent transcriptional regulation by PDX1

Emery T. Usher, Scott A. Showalter, 2022, Journal of Biological Chemistry

Probing multiple enzymatic methylation events in real time with NMR spectroscopy

Emery T. Usher, Kevin E.W. Namitz, Michael S. Cosgrove, Scott A. Showalter, 2021, Biophysical Journal on p. 4710-4721

Transient Electrostatic Interactions between Fcp1 and Rap74 Bias the Conformational Ensemble of the Complex with Minimal Impact on Binding Affinity

Victor A. Prieto, Kevin E.W. Namitz, Scott A. Showalter, 2021, Journal of Physical Chemistry B on p. 10917-10927

Intrinsically disordered substrates dictate SPOP subnuclear localization and ubiquitination activity

Emery T. Usher, Nafiseh Sabri, Roman Rohac, Amie K. Boal, Tanja Mittag, Scott A. Showalter, 2021, Journal of Biological Chemistry

Mapping invisible epitopes by NMR spectroscopy

Emery T. Usher, Scott A. Showalter, 2020, Journal of Biological Chemistry on p. 17411-17412

Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures

Suzette A. Pabit, Yen Lin Chen, Emery T. Usher, Erik C. Cook, Lois Pollack, Scott A. Showalter, 2020, Biophysical Journal on p. 2524-2536

An adjunctive therapy administered with an antibiotic prevents enrichment of antibiotic-resistant clones of a colonizing opportunistic pathogen

Valerie J. Morley, Clare Kinnear, Derek G. Sim, Samantha N. Olson, Samantha Olsen, Lindsey Jackson, Elsa Hansen, Grace Usher, Scott A. Showalter, Manjunath Pai, Robert Woods, Andrew F. Read, 2020, eLife on p. 1-17

Reviving Protein-Observed <sup>19</sup>F Lineshape Analysis for Deep Insight into Protein-Ligand Binding Events

Scott A. Showalter, 2020, Biophysical Journal on p. 2333-2335

Ultrasound-Guided Cytosolic Protein Delivery via Transient Fluorous Masks

Janna Sloand, Janna Sloand^, Eric Cook, Theodore Nguyen, Scott A. Zinck, Tawanda Zimudzi, Erik C. Cook, Scott Zinck, S Showalter, Tawanda J. Zimudzi, Scott Schowalter, Scott A. Showalter, Julianna Simon, S Medina, Adam B. Glick, Julianna Simon, Scott Medina*, 2020, ACS Nano on p. 4061-4073

Solution Ensemble of the C-Terminal Domain from the Transcription Factor Pdx1 Resembles an Excluded Volume Polymer

Erik C. Cook, Debashish Sahu, Monique Bastidas, Scott A. Showalter, 2019, Journal of Physical Chemistry B on p. 106-116

Most-Cited Papers

Application of NMR to studies of intrinsically disordered proteins

Eric B. Gibbs, Erik C. Cook, Scott A. Showalter, 2017, Archives of Biochemistry and Biophysics on p. 57-70

Structural Basis for Rare Earth Element Recognition by Methylobacterium extorquens Lanmodulin

Erik C. Cook, Emily R. Featherston, Scott A. Showalter, Joseph A. Cotruvo, 2019, Biochemistry on p. 120-125

Phosphorylation induces sequence-specific conformational switches in the RNA polymerase II C-terminal domain

Eric B. Gibbs, Feiyue Lu, Bede Portz, Michael J. Fisher, Brenda P. Medellin, Tatiana N. Laremore, Yan Jessie Zhang, David S. Gilmour, Scott A. Showalter, 2017, Nature Communications

Quantitative biophysical characterization of intrinsically disordered proteins

Eric B. Gibbs, Scott A. Showalter, 2015, Biochemistry on p. 1314-1326

Generating NMR chemical shift assignments of intrinsically disordered proteins using carbon-detected NMR methods

Debashish Sahu, Monique Bastidas, Scott A. Showalter, 2014, Analytical Biochemistry on p. 17-25

Structural heterogeneity in the intrinsically disordered RNA polymerase II C-terminal domain

Bede Portz, Feiyue Lu, Eric B. Gibbs, Joshua E. Mayfield, M. Rachel Mehaffey, Yan Jessie Zhang, Jennifer S. Brodbelt, Scott A. Showalter, David S. Gilmour, 2017, Nature Communications

A primer for carbon-detected NMR applications to intrinsically disordered proteins in solution

Monique Bastidas, Eric B. Gibbs, Debashish Sahu, Scott A. Showalter, 2015, Concepts in Magnetic Resonance Part A: Bridging Education and Research on p. 54-66

Ensemble analysis of primary microRNA structure reveals an extensive capacity to deform near the drosha cleavage site

Kaycee A. Quarles, Debashish Sahu, Mallory A. Havens, Ellen R. Forsyth, Christopher Wostenberg, Michelle L. Hastings, Scott A. Showalter, 2013, Biochemistry on p. 795-807

Role of ordered proteins in the folding-upon-binding of intrinsically disordered proteins

Chad W. Lawrence, Sushant Kumar, William G. Noid, Scott A. Showalter, 2014, Journal of Physical Chemistry Letters on p. 833-838

Expanding the proteome of an RNA virus by phosphorylation of an intrinsically disordered viral protein

Daniel G. Cordek, Tayler J. Croom-Perez, Jungwook Hwang, Michele R.S. Hargittai, Chennareddy V. Subba-Reddy, Qingxia Han, Maria Fernanda Lodeiro, Gang Ning, Thomas S. McCrory, Jamie J. Arnold, Hasan Koc, Brett D. Lindenbach, Scott A. Showalter, Craig E. Cameron, 2014, Journal of Biological Chemistry on p. 24397-24416