Mark Hedglin

Assistant Professor of Chemistry; Assistant Professor of Biochemistry and Molecular Biology

Mark Hedglin

Research Summary

Deciphering how efficient and faithful replication of the human genome is achieved within the highly-complex, dynamic, and reactive environment of the nucleus. Identifying pathways for genomic instability in humans, identifying novel oncogenic drug targets, developing better chemotherapeutic treatments for human cancers caused by genomic instability.

Huck Affiliations

Publication Tags

Dna Proliferating Cell Nuclear Antigen Replication Protein A Protein Antigen Dna Directed Dna Polymerase Proteins Holoenzymes Single Stranded Dna Clamping Devices Damage Tolerance Binding Sites Mutation Cell Viability Diffusion Haemophilus Influenzae Type B Polysaccharide Vaccine Diphtheria Protein Binding Replication Protein C Antineoplastic Agent Genes Dna Damage Modification Ubiquitin Dna Replication Loaders

Most Recent Publications

Tracking of progressing human DNA polymerase δ holoenzymes reveals distributions of DNA lesion bypass activities

Rachel L. Dannenberg, Joseph A. Cardina, Kara G. Pytko, Mark Hedglin, 2022, Nucleic Acids Research on p. 9893-9908

Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch

Christl Gaubitz, Xingchen Liu, Joseph Magrino, Nicholas P. Stone, Jacob Landeck, Mark Hedglin, Brian A. Kelch, 2020, Proceedings of the National Academy of Sciences of the United States of America on p. 23571-23580

Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase

Yeonoh Shin, Mark Hedglin, Katsuhiko S. Murakami, 2020, Nucleic Acids Research on p. 2144-2155

Rad6/Rad18 Competes with DNA Polymerases ν and δfor PCNA Encircling DNA

Mingjie Li, Leah Larsen, Mark Hedglin, 2020, Biochemistry on p. 407-416

PCNA Monoubiquitination Is Regulated by Diffusion of Rad6/Rad18 Complexes along RPA Filaments

Mingjie Li, Bhaswati Sengupta, Stephen J. Benkovic, Tae Hee Lee, Mark Hedglin, 2020, Biochemistry on p. 4694-4702

Rad6/Rad18 competes with DNA polymerases ? and ? for PCNA encircling DNA

Mingjie Li, Leah Larsen, Mark Hedglin, 2019, Biochemistry on p. 407-416

Replication protein A dynamically regulates monoubiquitination of proliferating cell nuclear antigen

Mark Hedglin, Mahesh Aitha, Anthony Pedley, Stephen Benkovic, 2019, Journal of Biological Chemistry on p. 5157-5168

RPA Dynamically Regulates Monoubiquitination of PCNA by Rad6/Rad18

Mark Hedglin, Mahesh Aitha, Anthony Pedley, S Benkovic, 2019, JOURNAL OF BIOLOGICAL CHEMISTRY on p. 20

Monitoring the Retention of Human Proliferating Cell Nuclear Antigen at Primer/Template Junctions by Proteins That Bind Single-Stranded DNA

Mark Hedglin, Mahesh Aitha, Stephen J. Benkovic, 2017, Biochemistry on p. 3415-3421

Eukaryotic Translesion DNA Synthesis on the Leading and Lagging Strands: Unique Detours around the Same Obstacle

Mark Hedglin, Stephen J. Benkovic, 2017, Chemical Reviews on p. 7857-7877

Most-Cited Papers

Replication clamps and clamp loaders

Mark Hedglin, Ravindra Kumar, Stephen J. Benkovic, 2013, Cold Spring Harbor perspectives in biology on p. 1-19

Regulation of Rad6/Rad18 Activity during DNA Damage Tolerance

Mark Hedglin, Stephen J. Benkovic, 2015, Annual Review of Biophysics and Biomolecular Structure on p. 207-228

Stability of the human polymerase δ holoenzyme and its implications in lagging strand DNA synthesis

Mark Hedglin, Binod Pandey, Stephen J. Benkovic, 2016, Proceedings of the National Academy of Sciences of the United States of America on p. E1777-E1786

Characterization of human translesion DNA synthesis across a UV-induced DNA lesion

Mark Hedglin, Binod Pandey, Stephen J. Benkovic, 2016, eLife

Eukaryotic Translesion DNA Synthesis on the Leading and Lagging Strands: Unique Detours around the Same Obstacle

Mark Hedglin, Stephen J. Benkovic, 2017, Chemical Reviews on p. 7857-7877

Isolating contributions from intersegmental transfer to DNA searching by alkyladenine DNA glycosylase

Mark Hedglin, Yaru Zhang, Patrick J. O'Brien, 2013, Journal of Biological Chemistry on p. 24550-24559

Stepwise assembly of the human replicative polymerase holoenzyme

Mark Hedglin, Senthil K. Perumal, Zhenxin Hu, Stephen Benkovic, 2013, eLife

Replication protein A dynamically regulates monoubiquitination of proliferating cell nuclear antigen

Mark Hedglin, Mahesh Aitha, Anthony Pedley, Stephen Benkovic, 2019, Journal of Biological Chemistry on p. 5157-5168

Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch

Christl Gaubitz, Xingchen Liu, Joseph Magrino, Nicholas P. Stone, Jacob Landeck, Mark Hedglin, Brian A. Kelch, 2020, Proceedings of the National Academy of Sciences of the United States of America on p. 23571-23580

Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA

Mark Hedglin, Stephen J. Benkovic, 2017, Biochemistry on p. 1824-1835