Mark Hedglin

Assistant Professor of Chemistry; Assistant Professor of Biochemistry and Molecular Biology

Mark Hedglin

Research Summary

Deciphering how efficient and faithful replication of the human genome is achieved within the highly-complex, dynamic, and reactive environment of the nucleus. Identifying pathways for genomic instability in humans, identifying novel oncogenic drug targets, developing better chemotherapeutic treatments for human cancers caused by genomic instability.

Huck Graduate Students

Huck Affiliations

Publication Tags

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Dna Proliferating Cell Nuclear Antigen Protein Antigen Replication Protein A Single Stranded Dna Proteins Dna Directed Dna Polymerase Diffusion Nucleotides Binding Sites Damage Tolerance Dna Damage Holoenzymes Haemophilus Influenzae Type B Polysaccharide Vaccine Diphtheria Amino Acids Replication Protein C Filament Modification Clamping Devices Nucleotide Ubiquitin Cell Viability Mutation Dna Binding Proteins

Most Recent Publications

PARP2 promotes Break Induced Replication-mediated telomere fragility in response to replication stress

Daniela Muoio, Natalie Laspata, Rachel L. Dannenberg, Caroline Curry, Simone Darkoa-Larbi, Mark Hedglin, Shikhar Uttam, Elise Fouquerel, 2024, Nature Communications

Replication of [AT/TA]<sub>25</sub> Microsatellite Sequences by Human DNA Polymerase δ Holoenzymes Is Dependent on dNTP and RPA Levels

Kara G. Pytko, Rachel L. Dannenberg, Kristin A. Eckert, Mark Hedglin, 2024, Biochemistry

Stepwise requirements for polymerases δ and θ in theta-mediated end joining

Susanna Stroik, Juan Carvajal-Garcia, Dipika Gupta, Alyssa Edwards, Adam Luthman, David W. Wyatt, Rachel L. Dannenberg, Wanjuan Feng, Thomas A. Kunkel, Gaorav P. Gupta, Mark Hedglin, Richard Wood, Sylvie Doublié, Eli Rothenberg, Dale A. Ramsden, 2023, Nature on p. 836-841

Mechanistic insight into AP-endonuclease 1 cleavage of abasic sites at stalled replication fork mimics

Nicole M. Hoitsma, Jessica Norris, Thu H. Khoang, Vikas Kaushik, Rahul Chadda, Edwin Antony, Mark Hedglin, Bret D. Freudenthal, 2023, Nucleic Acids Research on p. 6738-6753

Tracking of progressing human DNA polymerase δ holoenzymes reveals distributions of DNA lesion bypass activities

Rachel L. Dannenberg, Joseph A. Cardina, Kara G. Pytko, Mark Hedglin, 2022, Nucleic Acids Research on p. 9893-9908

Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch

Christl Gaubitz, Xingchen Liu, Joseph Magrino, Nicholas P. Stone, Jacob Landeck, Mark Hedglin, Brian A. Kelch, 2020, Proceedings of the National Academy of Sciences of the United States of America on p. 23571-23580

Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase

Yeonoh Shin, Mark Hedglin, Katsuhiko S. Murakami, 2020, Nucleic Acids Research on p. 2144-2155

Rad6/Rad18 Competes with DNA Polymerases ν and δfor PCNA Encircling DNA

Mingjie Li, Leah Larsen, Mark Hedglin, 2020, Biochemistry on p. 407-416

PCNA Monoubiquitination Is Regulated by Diffusion of Rad6/Rad18 Complexes along RPA Filaments

Mingjie Li, Bhaswati Sengupta, Stephen J. Benkovic, Tae Hee Lee, Mark Hedglin, 2020, Biochemistry on p. 4694-4702

Rad6/Rad18 competes with DNA polymerases δ and η for PCNA encircling DNA

Mingjie Li, Leah Larsen, Mark Hedglin, 2019, Biochemistry on p. 407-416

Most-Cited Papers

Regulation of Rad6/Rad18 Activity during DNA Damage Tolerance

Mark Hedglin, Stephen J. Benkovic, 2015, Annual Review of Biophysics and Biomolecular Structure on p. 207-228

Stability of the human polymerase δ holoenzyme and its implications in lagging strand DNA synthesis

Mark Hedglin, Binod Pandey, Stephen J. Benkovic, 2016, Proceedings of the National Academy of Sciences of the United States of America on p. E1777-E1786

Characterization of human translesion DNA synthesis across a UV-induced DNA lesion

Mark Hedglin, Binod Pandey, Stephen J. Benkovic, 2016, eLife

Eukaryotic Translesion DNA Synthesis on the Leading and Lagging Strands: Unique Detours around the Same Obstacle

Mark Hedglin, Stephen J. Benkovic, 2017, Chemical Reviews on p. 7857-7877

Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch

Christl Gaubitz, Xingchen Liu, Joseph Magrino, Nicholas P. Stone, Jacob Landeck, Mark Hedglin, Brian A. Kelch, 2020, Proceedings of the National Academy of Sciences of the United States of America on p. 23571-23580

Replication protein A dynamically regulates monoubiquitination of proliferating cell nuclear antigen

Mark Hedglin, Mahesh Aitha, Anthony Pedley, Stephen Benkovic, 2019, Journal of Biological Chemistry on p. 5157-5168

Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA

Mark Hedglin, Stephen J. Benkovic, 2017, Biochemistry on p. 1824-1835

Probing the DNA structural requirements for facilitated diffusion

Mark Hedglin, Yaru Zhang, Patrick J. O'Brien, 2015, Biochemistry on p. 557-566

Monitoring the Retention of Human Proliferating Cell Nuclear Antigen at Primer/Template Junctions by Proteins That Bind Single-Stranded DNA

Mark Hedglin, Mahesh Aitha, Stephen J. Benkovic, 2017, Biochemistry on p. 3415-3421

PCNA Monoubiquitination Is Regulated by Diffusion of Rad6/Rad18 Complexes along RPA Filaments

Mingjie Li, Bhaswati Sengupta, Stephen J. Benkovic, Tae Hee Lee, Mark Hedglin, 2020, Biochemistry on p. 4694-4702