Ibrahim Moustafa

Assistant Research Professor of Biochemistry and Molecular Biology

Ibrahim Moustafa

Research Summary

Process and analyze cryo-EM data to generate high-resolution 3D reconstructions using established software packages.

Huck Affiliations

Links

Most Recent Publications

Induced copy-back RNA synthesis as a therapeutic mechanism against RNA viruses

Ibrahim Moustafa, bioRxiv

Atomic-resolution structure of a chimeric Powassan tick-borne flavivirus

Sayan Das, Anoop Narayanan, Anqi Wang, Ibrahim M. Moustafa, Sung Hyun Cho, Dana Mitzel, Joyce Jose, Susan Hafenstein, 2025, Science advances

Mechanism of Forced-Copy-Choice RNA Recombination by Enteroviral RNA-Dependent RNA Polymerases

Jamie J. Arnold, Alexandre Martinez, Abha Jain, Xinran Liu, Ibrahim M. Moustafa, Craig E. Cameron, 2025, ACS Bio and Med Chem Au on p. 427-446

Non-lytic spread of poliovirus requires the nonstructural protein 3CD

David Aponte-Diaz, Jayden M. Harris, Tongjia Ella Kang, Victoria Korboukh, Mohamad S. Sotoudegan, Jennifer Lynn Gray, Neela H. Yennawar, Ibrahim M. Moustafa, Andrew Macadam, Craig Eugene Cameron, 2025, mBio

Intranasal SARS-CoV-2 RBD decorated nanoparticle vaccine enhances viral clearance in the Syrian hamster model

Devanshi R. Patel, Allen M. Minns, Derek G. Sim, Cassandra J. Field, Abigail E. Kerr, Talia A. Heinly, Erin H. Luley, Randall M. Rossi, Carol M. Bator, Ibrahim M. Moustafa, Elizabeth B. Norton, Susan L. Hafenstein, Scott E. Lindner, Troy C. Sutton, 2024, Microbiology spectrum

Interfering with nucleotide excision by the coronavirus 3<sup>'</sup>-to-5<sup>'</sup> exoribonuclease

Rukesh Chinthapatla, Mohamad Sotoudegan, Pankaj Srivastava, Thomas K. Anderson, Ibrahim M. Moustafa, Kellan T. Passow, Samantha A. Kennelly, Ramkumar Moorthy, David Dulin, Joy Y. Feng, Daniel A. Harki, Robert N. Kirchdoerfer, Craig E. Cameron, Jamie J. Arnold, 2023, Nucleic Acids Research on p. 315-336

Interfering with nucleotide excision by the coronavirus 3`-to-5` exoribonuclease

Ibrahim Moustafa, 2022, bioRxiv

Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses

Richard Janissen, Andrew Woodman, Djoshkun Shengjuler, Thomas Vallet, Kuo Ming Lee, Louis Kuijpers, Ibrahim M. Moustafa, Fiona Fitzgerald, Peng Nien Huang, Angela L. Perkins, Daniel A. Harki, Jamie J. Arnold, Belén Solano, Shin Ru Shih, Marco Vignuzzi, Craig E. Cameron, Nynke H. Dekker, 2021, Molecular Cell on p. 4467-4480.e7

Identification of a pocket factor that is critical to Zika virus assembly

Nadia M. DiNunno, Daniel J. Goetschius, Anoop Narayanan, Sydney A. Majowicz, Ibrahim Moustafa, Carol M. Bator, Susan L. Hafenstein, Joyce Jose, 2020, Nature Communications

Rational Control of Poliovirus RNA-Dependent RNA Polymerase Fidelity by Modulating Motif-D Loop Conformational Dynamics

Jingjing Shi, Jacob M. Perryman, Xiaorong Yang, Xinran Liu, Derek M. Musser, Alyson K. Boehr, Ibrahim M. Moustafa, Jamie J. Arnold, Craig E. Cameron, David D. Boehr, 2019, Biochemistry on p. 3735-3743

Most-Cited Papers

Sequence-Specific Fidelity Alterations Associated with West Nile Virus Attenuation in Mosquitoes

Greta A. Van Slyke, Jamie J. Arnold, Alex J. Lugo, Sara B. Griesemer, Ibrahim M. Moustafa, Laura D. Kramer, Craig E. Cameron, Alexander T. Ciota, 2015, PLoS Pathogens

Identification of a pocket factor that is critical to Zika virus assembly

Nadia M. DiNunno, Daniel J. Goetschius, Anoop Narayanan, Sydney A. Majowicz, Ibrahim Moustafa, Carol M. Bator, Susan L. Hafenstein, Joyce Jose, 2020, Nature Communications

The RNA-Binding Site of Poliovirus 3C Protein Doubles as a Phosphoinositide-Binding Domain

Djoshkun Shengjuler, Yan Mei Chan, Simou Sun, Ibrahim M. Moustafa, Zhen Lu Li, David W. Gohara, Matthias Buck, Paul S. Cremer, David D. Boehr, Craig E. Cameron, 2017, Structure with Folding & design on p. 1875-1886.e7

Fidelity of Nucleotide Incorporation by the RNA-Dependent RNA Polymerase from Poliovirus

C. E. Cameron, I. M. Moustafa, J. J. Arnold, 2016, DNA REPLICATION ACROSS TAXA on p. 293--323

Rational Control of Poliovirus RNA-Dependent RNA Polymerase Fidelity by Modulating Motif-D Loop Conformational Dynamics

Jingjing Shi, Jacob M. Perryman, Xiaorong Yang, Xinran Liu, Derek M. Musser, Alyson K. Boehr, Ibrahim M. Moustafa, Jamie J. Arnold, Craig E. Cameron, David D. Boehr, 2019, Biochemistry on p. 3735-3743

Interfering with nucleotide excision by the coronavirus 3<sup>'</sup>-to-5<sup>'</sup> exoribonuclease

Rukesh Chinthapatla, Mohamad Sotoudegan, Pankaj Srivastava, Thomas K. Anderson, Ibrahim M. Moustafa, Kellan T. Passow, Samantha A. Kennelly, Ramkumar Moorthy, David Dulin, Joy Y. Feng, Daniel A. Harki, Robert N. Kirchdoerfer, Craig E. Cameron, Jamie J. Arnold, 2023, Nucleic Acids Research on p. 315-336

Triphosphate reorientation of the incoming nucleotide as a fidelity checkpoint in viral RNA-dependent RNA Polymerases

Xiaorong Yang, Xinran Liu, Derek M. Musser, Ibrahim M. Moustafa, Jamie J. Arnold, Craig E. Cameron, David D. Boehr, 2017, Journal of Biological Chemistry on p. 3810-3826

Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses

Richard Janissen, Andrew Woodman, Djoshkun Shengjuler, Thomas Vallet, Kuo Ming Lee, Louis Kuijpers, Ibrahim M. Moustafa, Fiona Fitzgerald, Peng Nien Huang, Angela L. Perkins, Daniel A. Harki, Jamie J. Arnold, Belén Solano, Shin Ru Shih, Marco Vignuzzi, Craig E. Cameron, Nynke H. Dekker, 2021, Molecular Cell on p. 4467-4480.e7

Conformational ensemble of the poliovirus 3CD precursor observed by MD simulations and confirmed by SAXS: A strategy to expand the viral proteome?

Ibrahim M. Moustafa, D Gohara, A Uchida, Neela Yennawar, C Cameron, 2015, Viruses on p. 5962-5986

Long-Range Communication between Different Functional Sites in the Picornaviral 3C Protein

Yan M. Chan, Ibrahim M. Moustafa, Jamie J. Arnold, Craig E. Cameron, David D. Boehr, 2016, Structure with Folding & design on p. 509-517