Ibrahim Moustafa

Assistant Research Professor of Biochemistry and Molecular Biology

W231 Millennium Science Complex
University Park, PA
ria2@psu.edu
814-865-5733

Research Summary

Process and analyze cryo-EM data to generate high-resolution 3D reconstructions using established software packages.

Huck Affiliations

Cryo-Electron Microscopy Facility

Center for Structural Biology

Most Recent Publications

Induced copy-back RNA synthesis as a therapeutic mechanism against RNA viruses

Ibrahim Moustafa, bioRxiv

Intranasal SARS-CoV-2 RBD decorated nanoparticle vaccine enhances viral clearance in the Syrian hamster model.

D Patel, A Minns, D Sim, C Field, A Kerr, T Heinly, E Luley, R Rossi, C Bator, I Moustafa, E Norton, S Hafenstein, S Lindner, Troy Sutton, 2024, Microbiology spectrum on p. e0499822

Intranasal SARS-CoV-2 RBD decorated nanoparticle vaccine enhances viral clearance in the Syrian hamster model

Devanshi R. Patel, Allen M. Minns, Derek G. Sim, Cassandra J. Field, Abigail E. Kerr, Talia A. Heinly, Erin H. Luley, Randall M. Rossi, Carol M. Bator, Ibrahim M. Moustafa, Elizabeth B. Norton, Susan L. Hafenstein, Scott E. Lindner, Troy C. Sutton, 2024, Microbiology spectrum

Interfering with nucleotide excision by the coronavirus 3<sup>'</sup>-to-5<sup>'</sup> exoribonuclease

Rukesh Chinthapatla, Mohamad Sotoudegan, Pankaj Srivastava, Thomas K. Anderson, Ibrahim M. Moustafa, Kellan T. Passow, Samantha A. Kennelly, Ramkumar Moorthy, David Dulin, Joy Y. Feng, Daniel A. Harki, Robert N. Kirchdoerfer, Craig E. Cameron, Jamie J. Arnold, 2023, Nucleic Acids Research on p. 315-336

Interfering with nucleotide excision by the coronavirus 3`-to-5` exoribonuclease

Ibrahim Moustafa, 2022, bioRxiv

Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses

Richard Janissen, Andrew Woodman, Djoshkun Shengjuler, Thomas Vallet, Kuo Ming Lee, Louis Kuijpers, Ibrahim M. Moustafa, Fiona Fitzgerald, Peng Nien Huang, Angela L. Perkins, Daniel A. Harki, Jamie J. Arnold, Belén Solano, Shin Ru Shih, Marco Vignuzzi, Craig E. Cameron, Nynke H. Dekker, 2021, Molecular Cell on p. 4467-4480.e7

Identification of a pocket factor that is critical to Zika virus assembly

Nadia M. DiNunno, Daniel J. Goetschius, Anoop Narayanan, Sydney A. Majowicz, Ibrahim Moustafa, Carol M. Bator, Susan L. Hafenstein, Joyce Jose, 2020, Nature Communications

Rational Control of Poliovirus RNA-Dependent RNA Polymerase Fidelity by Modulating Motif-D Loop Conformational Dynamics

Jingjing Shi, Jacob M. Perryman, Xiaorong Yang, Xinran Liu, Derek M. Musser, Alyson K. Boehr, Ibrahim M. Moustafa, Jamie J. Arnold, Craig E. Cameron, David D. Boehr, 2019, Biochemistry on p. 3735-3743

Molecular dynamics simulations suggest changes in electrostatic interactions as a potential mechanism through which serine phosphorylation inhibits DNA polymerase β activity

Dirar Homouz, Kwee Hong Joyce-Tan, Mohd ShahirShamsir, Ibrahim M. Moustafa, Haitham T. Idriss, 2018, Journal of Molecular Graphics and Modelling on p. 236-241

Molecular dynamics simulations suggest changes in electrostatic interactions as a potential mechanism through which serine phosphorylation inhibits DNA polymerase β activity.

D Homouz, K Joyce-Tan, M ShahirShamsir, Ibrahim Moustafa, H Idriss, 2018, Journal of molecular graphics & modelling on p. 236-241

Most-Cited Papers

Sequence-Specific Fidelity Alterations Associated with West Nile Virus Attenuation in Mosquitoes

Greta A. Van Slyke, Jamie J. Arnold, Alex J. Lugo, Sara B. Griesemer, Ibrahim M. Moustafa, Laura D. Kramer, Craig E. Cameron, Alexander T. Ciota, 2015, PLoS Pathogens

Structural dynamics as a contributor to error-prone replication by an RNA-dependent RNA polymerase

Ibrahim M. Moustafa, Victoria K. Korboukh, Jamie J. Arnold, Eric D. Smidansky, Laura L. Marcotte, David W. Gohara, Xiaorong Yang, María Antonieta Saánchez-Farraán, David Filman, Janna K. Maranas, David D. Boehr, James M. Hogle, Coray M. Colina, Craig E. Cameron, 2014, Journal of Biological Chemistry on p. 36229-36248

Identification of a pocket factor that is critical to Zika virus assembly

Nadia M. DiNunno, Daniel J. Goetschius, Anoop Narayanan, Sydney A. Majowicz, Ibrahim Moustafa, Carol M. Bator, Susan L. Hafenstein, Joyce Jose, 2020, Nature Communications

Fidelity of Nucleotide Incorporation by the RNA-Dependent RNA Polymerase from Poliovirus

C. E. Cameron, I. M. Moustafa, J. J. Arnold, 2016, DNA REPLICATION ACROSS TAXA on p. 293--323

The RNA-Binding Site of Poliovirus 3C Protein Doubles as a Phosphoinositide-Binding Domain

Djoshkun Shengjuler, Yan Mei Chan, Simou Sun, Ibrahim M. Moustafa, Zhen Lu Li, David W. Gohara, Matthias Buck, Paul S. Cremer, David D. Boehr, Craig E. Cameron, 2017, Structure with Folding & design on p. 1875-1886.e7

Triphosphate reorientation of the incoming nucleotide as a fidelity checkpoint in viral RNA-dependent RNA Polymerases

Xiaorong Yang, Xinran Liu, Derek M. Musser, Ibrahim M. Moustafa, Jamie J. Arnold, Craig E. Cameron, David D. Boehr, 2017, Journal of Biological Chemistry on p. 3810-3826

Rational Control of Poliovirus RNA-Dependent RNA Polymerase Fidelity by Modulating Motif-D Loop Conformational Dynamics

Conformational ensemble of the poliovirus 3CD precursor observed by MD simulations and confirmed by SAXS: A strategy to expand the viral proteome?

Ibrahim M. Moustafa, David W. Gohara, Akira Uchida, Neela Yennawar, Craig E. Cameron, 2015, Viruses on p. 5962-5986

Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses

Long-Range Communication between Different Functional Sites in the Picornaviral 3C Protein

Yan M. Chan, Ibrahim M. Moustafa, Jamie J. Arnold, Craig E. Cameron, David D. Boehr, 2016, Structure with Folding & design on p. 509-517

News Articles Featuring Ibrahim Moustafa

Mar 12, 2024

New nasal vaccine platform helps clear COVID-19 infections in an animal model

A newly developed intranasal vaccine candidate helps to clear COVID-19 infections more quickly than controls in pre-clinical testing, according to a recent study.

Full Article