B. Tracy Nixon

Professor of Biochemistry and Molecular Biology

B. Tracy Nixon

Research Summary

Structural and functional basis of cellulose synthesis. Using Physcomitrella patens and other organisms as model systems, we are learning how plants make cellulose for building new cell wall. The studies use methods of molecular biology and cryoEM to characterize the enzyme as a monomer, and when it assembles into its larger 'Cellulose Synthase Complex '(CSC for short). The aim is to understand cellulose synthesis to explain fundamentals of cell wall biology in plants, and to enable manipulation of its synthesis for applications in fields of bioenergy and materials.

Huck Affiliations

Links

Publication Tags

Cellulose Cellulose Synthase Proteins Microfibrils Synthesis Membrane Proteins In Vitro Techniques Adenosine Triphosphate Adenosine Triphosphatases Arabidopsis Cell Walls Bacteria Crystallization Adenosine Diphosphate Cell Wall Membranes X Rays Nucleotides Hydrolysis Bryopsida Cellulose Microfibrils Dna Directed Rna Polymerases Rhodobacter Sphaeroides Transmission Electron Microscopy Coloring Agents

Most Recent Papers

Synthesis and self-assembly of cellulose microfibrils from reconstituted cellulose synthase

Sung Hyun Cho, Pallinti Purushotham, Chao Fang, Cassandra Maranas, Sara M. Díaz-Moreno, Vincent Bulone, Jochen Zimmer, Manish Kumar, B. Tracy Nixon, 2017, Plant Physiology on p. 146-156

A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro

Pallinti Purushotham, Sung Hyun Cho, Sara M. Díaz-Moreno, Manish Kumar, B. Tracy Nixon, Vincent Bulone, Jochen Zimmer, 2016, Proceedings of the National Academy of Sciences of the United States of America on p. 11360-11365

Comparative Structural and Computational Analysis Supports Eighteen Cellulose Synthases in the Plant Cellulose Synthesis Complex

B. Tracy Nixon, Katayoun Mansouri, Abhishek Singh, Juan Du, Jonathan K. Davis, Jung Goo Lee, Erin Slabaugh, Venu Gopal Vandavasi, Hugh O'Neill, Eric M. Roberts, Alison W. Roberts, Yaroslava G. Yingling, Candace H. Haigler, 2016, Scientific Reports

Structure of the cellulose synthase complex of Gluconacetobacter hansenii at 23.4 Å resolution

Juan Du, Venkata Vepachedu, Sung Hyun Cho, Manish Kumar, B. Tracy Nixon, 2016, PLoS One

The Enzymatic Conversion of Major Algal and Cyanobacterial Carbohydrates to Bioethanol

Al Abdallah, Qusai, B. Tracy Nixon, Jarrod Fortwendel, 2016, FRONTIERS IN ENERGY RESEARCH

A structural study of CESA1 catalytic domain of arabidopsis cellulose synthesis complex

Venu Gopal Vandavasi, Daniel K. Putnam, Qiu Zhang, Loukas Petridis, William T. Heller, B. Tracy Nixon, Candace H. Haigler, Udaya Kalluri, Leighton Coates, Paul Langan, Jeremy C. Smith, Jens Meiler, Hugh O’Neill, 2016, Plant Physiology on p. 123-135

Advancing Rhodobacter sphaeroides as a platform for expression of functional membrane proteins

Mustafa Erbakan, Brandon S. Curtis, B. Tracy Nixon, Manish Kumar, Wayne R. Curtis, 2015, Protein Expression and Purification on p. 109-117

In vitro synthesis of cellulose microfibrils by a membrane protein from protoplasts of the non-vascular plant Physcomitrella patens

Sung Hyun Cho, Juan Du, Ian Sines, Venkata Giridhar Poosarla, Venkata Vepachedu, Kabindra Kafle, Yong Bum Park, Seong Kim, Manish Kumar, B. Tracy Nixon, 2015, Biochemical Journal on p. 195-205

Crystallization and preliminary X-ray analysis of the ATPase domain of the σ<sup>54</sup>-dependent transcription activator NtrC1 from aquifex aeolicus bound to the ATP analog ADP-BeF<sub>x</sub>

Tatyana A. Sysoeva, Neela Yennawar, Marc Allaire, B. Tracy Nixon, 2013, Acta Crystallographica Section F:Structural Biology Communications on p. 1384-1388

Nucleotide-induced asymmetry within ATPase activator ring drives Σ54-RNAP interaction and ATP hydrolysis

Tatyana A. Sysoeva, Saikat Chowdhury, Liang Guo, B. Tracy Nixon, 2013, Genes and Development on p. 2500-2511

Most-Cited Papers

Phase transitions in the assembly of multivalent signalling proteins

Pilong Li, Sudeep Banjade, Hui Chun Cheng, Soyeon Kim, Baoyu Chen, Liang Guo, Marc Llaguno, Javoris V. Hollingsworth, David S. King, Salman F. Banani, Paul S. Russo, Qiu Xing Jiang, B. Tracy Nixon, Michael K. Rosen, 2012, Nature on p. 336-340

Opening and closing of the bacterial RNA polymerase clamp

Anirban Chakraborty, Dongye Wang, Yon W. Ebright, You Korlann, Ekaterine Kortkhonjia, Taiho Kim, Saikat Chowdhury, Sivaramesh Wigneshweraraj, Herbert Irschik, Rolf Jansen, B. Tracy Nixon, Jennifer Knight, Shimon Weiss, Richard H. Ebright, 2012, Science on p. 591-595

Comparative Structural and Computational Analysis Supports Eighteen Cellulose Synthases in the Plant Cellulose Synthesis Complex

B. Tracy Nixon, Katayoun Mansouri, Abhishek Singh, Juan Du, Jonathan K. Davis, Jung Goo Lee, Erin Slabaugh, Venu Gopal Vandavasi, Hugh O'Neill, Eric M. Roberts, Alison W. Roberts, Yaroslava G. Yingling, Candace H. Haigler, 2016, Scientific Reports

A structural study of CESA1 catalytic domain of arabidopsis cellulose synthesis complex

Venu Gopal Vandavasi, Daniel K. Putnam, Qiu Zhang, Loukas Petridis, William T. Heller, B. Tracy Nixon, Candace H. Haigler, Udaya Kalluri, Leighton Coates, Paul Langan, Jeremy C. Smith, Jens Meiler, Hugh O’Neill, 2016, Plant Physiology on p. 123-135

A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro

Pallinti Purushotham, Sung Hyun Cho, Sara M. Díaz-Moreno, Manish Kumar, B. Tracy Nixon, Vincent Bulone, Jochen Zimmer, 2016, Proceedings of the National Academy of Sciences of the United States of America on p. 11360-11365

Nucleotide-induced asymmetry within ATPase activator ring drives Σ54-RNAP interaction and ATP hydrolysis

Tatyana A. Sysoeva, Saikat Chowdhury, Liang Guo, B. Tracy Nixon, 2013, Genes and Development on p. 2500-2511

Synthesis and self-assembly of cellulose microfibrils from reconstituted cellulose synthase

Sung Hyun Cho, Pallinti Purushotham, Chao Fang, Cassandra Maranas, Sara M. Díaz-Moreno, Vincent Bulone, Jochen Zimmer, Manish Kumar, B. Tracy Nixon, 2017, Plant Physiology on p. 146-156

Structure of the cellulose synthase complex of Gluconacetobacter hansenii at 23.4 Å resolution

Juan Du, Venkata Vepachedu, Sung Hyun Cho, Manish Kumar, B. Tracy Nixon, 2016, PLoS One

In vitro synthesis of cellulose microfibrils by a membrane protein from protoplasts of the non-vascular plant Physcomitrella patens

Sung Hyun Cho, Juan Du, Ian Sines, Venkata Giridhar Poosarla, Venkata Vepachedu, Kabindra Kafle, Yong Bum Park, Seong Kim, Manish Kumar, B. Tracy Nixon, 2015, Biochemical Journal on p. 195-205

Advancing Rhodobacter sphaeroides as a platform for expression of functional membrane proteins

Mustafa Erbakan, Brandon S. Curtis, B. Tracy Nixon, Manish Kumar, Wayne R. Curtis, 2015, Protein Expression and Purification on p. 109-117