Overview and a new method for IEM without detergent permeabilization

Immunogold labeling combined with electron microscopy is a powerful tool in biomedical research for localization of specific molecules with ultrastructural resolution in cells and tissues. However, technical challenges resulting from labor-intensive and expensive procedures, together with limited success rates, have prevented its application by many researchers. Sponsored by a Huck Facility pilot grant, Rick Ordway s research group has developed and implemented a new IEM method for his research in neuroscience, where traditionally-used detergent treatment of tissue is not employed. This mini-workshop aims to provide an overview of the principles and methods of IEM, and it will introduce this important novel procedure which facilitates preservation of ultrastructure by eliminating detergent treatment of tissue samples. Time and Schedule**: Wednesday, July 9, 2014 10:00 - 10:50 Introduction and Overview. Greg Ning 10:50 - 11:10 Coffee break 11:10 - 12:00 Pre-embedding IEM: A New Method without Detergent Permeabilization and its Applications. Rick Ordway 12:00 - 12:30 Round-table Discussion Place: MSC W203 Instructors: Rick Ordway, Greg Ning, Fumi Kawasaki Refreshments will be served. Registration: Attendance is FREE! However, registration is required for head-counting in advance (for the refreshments!). Please send an email to Julia Fecko (jaf48@psu.edu). We look forward to seeing you. **Prior to this workshop there is a Huck Microscopy and Cytometry Facility s Monthly Training session from 9:00 - 9:50AM.

Contact

  Greg Ning
  gxn7@psu.edu
  814-863-0994