The FEI Titan Krios is an instrument that realizes the revolutionary developments in cryo-EM leading to the “resolution revolution” that allows atomic resolution maps of biological objects with low or no symmetry. These transformative advances include the exceptionally stable microscope stage, automated data collection, direct electron detection technology, fast read out for data transfer. However, our Penn State Krios microscope is one of a kind.
Uses:
- Screening of proteins, viruses, nanoparticles, etc. at cryogenic temperature
- Data collection of single particles for 3D reconstruction
- Data collection for tomography
Specifications:
- Cs (spherical aberration) corrector
- Falcon 4i direct electron detector
- Selectris X energy filter
- Fringe-Free Imaging (FFI)
- Cryo-Flow for real-time quality check during data collection
- EPU software
- Tomography software
- CryoSparc-live
- The best resolution (1.46A) from apoferritin
Instrument/Service Location:
Cryo-Electron Microscopy Facility
How to Obtain Access:
Visit https://psu.corefacilities.org
How to Obtain Support:
Visit https://psu.corefacilities.org