Yuguo (Leo) Lei

Associate Professor of Biomedical Engineering, Faculty Oversight, Sartorius Cell Culture Facility

Yuguo (Leo) Lei

Research Summary

Cell therapy; Cell manufacturing; Biomaterials

Huck Affiliations


Publication Tags

Stem Cells Hydrogels Cell Culture Polyethylene Glycol Dimethacrylate Hydrogel Hydrogel Pluripotent Stem Cells Hyaluronic Acid Skin Muscle Cells Tissue Fibronectins Drug Cell Culture Techniques Dna Fibrin Costs Endothelial Cells Brain Nanofibers Collagen Fibrinogen Reaction Yield Scaffolds Genes

Most Recent Papers

Demonstration of re-epithelialization in a bioprinted human skin equivalent wound model

Carlos Poblete Jara, Carolina Motter Catarino, Yuguo Lei, Lício Augusto Velloso, Pankaj Karande, William H. Velander, Eliana Pereira de Araujo, 2021, Bioprinting


Yuguo Lei, Stephanie Michelle Willerth, Tiago G. Fernandes, 2021, Frontiers in Bioengineering and Biotechnology

Comparative Study of Human Pluripotent Stem Cell-Derived Endothelial Cells in Hydrogel-Based Culture Systems

Zhanqi Wang, Fuxing Zuo, Qing Liu, Xuesheng Wu, Qian Du, Yuguo Lei, Zhangmin Wu, Haishuang Lin, 2021, ACS Omega on p. 6942-6952

Amyloid precursor protein is a restriction factor that protects against Zika virus infection in mammalian brains

Amy Lingel, Haishuang Lin, Yuval Gavriel, Eric Weaver, Pascal Polepole, Virginia Lopez, Yuguo Lei, Thomas M. Petro, Beka Solomon, Chi Zhang, Luwen Zhang, 2020, Journal of Biological Chemistry on p. 17114-17127

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Carlos Poblete Jara, Ou Wang, Thais Paulino do Prado, Ayman Ismail, Frank Marco Fabian, Han Li, Licio A. Velloso, Mark A. Carlson, William Burgess, Yuguo Lei, William H. Velander, Eliana P. Araújo, 2020, Bioactive Materials on p. 949-962

Reversible associations between human plasma fibronectin and fibrinogen γγ’ heterodimer observed by high pressure size exclusion chromatography and dynamic light scattering

Frank M. Fabian, Ayman E.A. Ismail, Ou Wang, Yuguo Lei, William H. Velander, 2020, Analytical Biochemistry

Multi-scale modeling of the lamellar unit of arterial media

Hozhabr Mozafari, Lulu Wang, Yuguo Lei, Linxia Gu, 2020, Nanotechnology Reviews on p. 539-547

Engineered Microenvironment for Manufacturing Human Pluripotent Stem Cell-Derived Vascular Smooth Muscle Cells

Haishuang Lin, Xuefeng Qiu, Qian Du, Qiang Li, Ou Wang, Leonard Akert, Zhanqi Wang, Dirk Anderson, Kan Liu, Linxia Gu, Chi Zhang, Yuguo Lei, 2019, Stem Cell Reports on p. 84-97

Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels

Haishuang Lin, Qian Du, Qiang Li, Ou Wang, Zhanqi Wang, Kan Liu, Leonard Akert, Chi Zhang, Soonkyu Chung, Bin Duan, Yuguo Lei, 2019, Biomaterials Science on p. 347-361

Integrated generation of induced pluripotent stem cells in a low-cost device

Haishuang Lin, Qiang Li, Qian Du, Ou Wang, Zhanqi Wang, Leonard Akert, Mark A. Carlson, Chi Zhang, Anuradha Subramanian, Chi Zhang, Matthew Lunning, Ming Li, Yuguo Lei, 2019, Biomaterials on p. 23-36

Most-Cited Papers

A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation

Yuguo Lei, David V. Schaffer, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. E5039-E5048

Developing defined and scalable 3D culture systems for culturing human pluripotent stem cells at high densities

Yuguo Lei, Daeun Jeong, Jifang Xiao, David V. Schaffer, 2014, Cellular and Molecular Bioengineering on p. 172-183

Thermoreversible Hyaluronic Acid-PNIPAAm Hydrogel Systems for 3D Stem Cell Culture

Barbara L. Ekerdt, Christina M. Fuentes, Yuguo Lei, Maroof M. Adil, Anusuya Ramasubramanian, Rachel A. Segalman, David V. Schaffer, 2018, Advanced healthcare materials

Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs

Talar Tokatlian, Cynthia Cam, Shayne N. Siegman, Yuguo Lei, Tatiana Segura, 2012, Acta Biomaterialia on p. 3921-3931

Establishment of a Human iPSC- and Nanofiber-Based Microphysiological Blood-Brain Barrier System

Dianjun Qi, Shaohua Wu, Haishuang Lin, Mitchell A. Kuss, Yuguo Lei, Alexey Krasnoslobodtsev, Shaheen Ahmed, Chi Zhang, Hyung Joon Kim, Peng Jiang, Bin Duan, 2018, ACS applied materials & interfaces on p. 21825-21835

Three-dimensional tissues using human pluripotent stem cell spheroids as biofabrication building blocks

Haishuang Lin, Qiang Li, Yuguo Lei, 2017, Biofabrication

Scalable Production of Glioblastoma Tumor-initiating Cells in 3 Dimension Thermoreversible Hydrogels

Qiang Li, Haishuang Lin, Ou Wang, Xuefeng Qiu, Srivatsan Kidambi, Loic P. Deleyrolle, Brent A. Reynolds, Yuguo Lei, 2016, Scientific Reports

Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System

Qiang Li, Haishuang Lin, Jack Rauch, Loic P. Deleyrolle, Brent A. Reynolds, Hendrik J. Viljoen, Chi Zhang, Linxia Gu, Erika Van Wyk, Yuguo Lei, 2018, Scientific Reports

Effects of tunable, 3D-bioprinted hydrogels on human brown adipocyte behavior and metabolic function

Mitchell Kuss, Jiyoung Kim, Dianjun Qi, Shaohua Wu, Yuguo Lei, Soonkyu Chung, Bin Duan, 2018, Acta Biomaterialia on p. 486-495

Scalable and physiologically relevant microenvironments for human pluripotent stem cell expansion and differentiation

Qiang Li, Haishuang Lin, Qian Du, Kan Liu, Ou Wang, Catelyn Evans, Hannah Christian, Chi Zhang, Yuguo Lei, 2018, Biofabrication