Xiaojun (Lance) Lian

Associate Professor of Biomedical Engineering

Xiaojun (Lance) Lian

Research Summary

Human Stem Cell Engineering; Genome Editing via CRISPR-Cas9; Epigenome Editing and Epigenetics.

Huck Graduate Students

Huck Affiliations

Links

Publication Tags

These publication tags are generated from the output of this researcher. Click any tag below to view other Huck researchers working on the same topic.

Pluripotent Stem Cells Stem Cells Application Cardiac Myocytes Catenins Glucose Nanopores Mechanics Clustered Regularly Interspaced Short Palindromic Repeats Endothelial Cells Drug Transcription Factors Rna Sequencing Rna Beta Catenin Molecules Cell Differentiation Proteins Dna Messenger Rna Substrates Lasers Solid State Gene Editing Genomics

Most Recent Publications

Fabricating 3-dimensional human brown adipose microtissues for transplantation studies

Ou Wang, Li Han, Haishuang Lin, Mingmei Tian, Shuyang Zhang, Bin Duan, Soonkyu Chung, Chi Zhang, Xiaojun Lian, Yong Wang, Yuguo Lei, 2023, Bioactive Materials on p. 518-534

Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells

Tahir Haideri, Alessandro Howells, Yuqian Jiang, Jian Yang, Xiaoping Bao, Xiaojun Lance Lian, 2022, Cell Reports Methods

Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems

Yuqian Jiang, Rachel Catherine Hoenisch, Yun Chang, Xiaoping Bao, Craig E. Cameron, Xiaojun Lance Lian, 2022, Bioactive Materials on p. 313-320

Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy

Yun Chang, Ramizah Syahirah, Xuepeng Wang, Gyuhyung Jin, Sandra Torregrosa-Allen, Bennett D. Elzey, Sydney N. Hummel, Tianqi Wang, Can Li, Xiaojun Lian, Qing Deng, Hal E. Broxmeyer, Xiaoping Bao, 2022, Cell Reports

Temporal Expression of Transcription Factor ID2 Improves Natural Killer Cell Differentiation from Human Pluripotent Stem Cells

Juhyung Jung, Yun Chang, Gyuhyung Jin, Xiaojun Lian, Xiaoping Bao, 2022, ACS Synthetic Biology on p. 2001-2008

Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells

Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao, 2022, Biomaterials

A dual cardiomyocyte reporter model derived from human pluripotent stem cells

Yuqian Jiang, Xiaoping Bao, Xiaojun Lance Lian, 2021, Stem Cell Research and Therapy

Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores

Reza Nouri, Yuqian Jiang, Zifan Tang, Xiaojun Lance Lian, Weihua Guan, 2021, Nano Letters on p. 8393-8400

Generation of pancreatic progenitors from human pluripotent stem cells by small molecules

Yuqian Jiang, Chuanxin Chen, Lauren N. Randolph, Songtao Ye, Xin Zhang, Xiaoping Bao, Xiaojun Lance Lian, 2021, Stem Cell Reports on p. 2395-2409

Optogenetic-mediated cardiovascular differentiation and patterning of human pluripotent stem cells

Peter B. Hellwarth, Yun Chang, Arundhati Das, Po Yu Liang, Xiaojun Lian, Nicole A. Repina, Xiaoping Bao, 2021, Advanced Genetics

Most-Cited Papers

Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions

Xiaojun Lian, Jianhua Zhang, Samira M. Azarin, Kexian Zhu, Laurie B. Hazeltine, Xiaoping Bao, Cheston Hsiao, Timothy J. Kamp, Sean P. Palecek, 2013, Nature Protocols on p. 162-175

Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling

Xiaojun Lian, Xiaoping Bao, Abraham Al-Ahmad, Jialu Liu, Yue Wu, Wentao Dong, Kaitlin K. Dunn, Eric V. Shusta, Sean P. Palecek, 2014, Stem Cell Reports on p. 804-816

Chemically defined, albumin-free human cardiomyocyte generation

Xiaojun Lian, Xiaoping Bao, Misha Zilberter, Mattias Westman, André Fisahn, Cheston Hsiao, Laurie B. Hazeltine, Kaitlin K. Dunn, Timothy J. Kamp, Sean P. Palecek, 2015, Nature Methods on p. 595-596

Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions

Xiaoping Bao, Xiaojun Lian, Timothy A. Hacker, Eric G. Schmuck, Tongcheng Qian, Vijesh J. Bhute, Tianxiao Han, Mengxuan Shi, Lauren Drowley, Alleyn T. Plowright, Qing Dong Wang, Marie Jose Goumans, Sean P. Palecek, 2017, Nature Biomedical Engineering

Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells

Xiaoping Bao, Xiaojun Lian, Kaitlin K. Dunn, Mengxuan Shi, Tianxiao Han, Tongcheng Qian, Vijesh J. Bhute, Scott G. Canfield, Sean P. Palecek, 2015, Stem Cell Research on p. 122-129

Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)

Reza Nouri, Yuqian Jiang, Xiaojun Lance Lian, Weihua Guan, 2020, ACS Sensors on p. 1273-1280

Insulin inhibits cardiac mesoderm, not mesendoderm, formation during cardiac differentiation of human pluripotent stem cells and modulation of canonical wnt signaling can rescue this inhibition

Xiaojun Lian, Jianhua Zhang, Kexian Zhu, Timothy J. Kamp, Sean P. Palecek, 2013, Stem Cells on p. 447-457

Temporal impact of substrate mechanics on differentiation of human embryonic stem cells to cardiomyocytes

Laurie B. Hazeltine, Mehmet G. Badur, Xiaojun Lian, Amritava Das, Wenqing Han, Sean P. Palecek, 2014, Acta Biomaterialia on p. 604-612

Laser-induced graphene non-enzymatic glucose sensors for on-body measurements

Jian Yang, Jia Zhu, Shangbin Liu, Zhihui Hu, Xianzhe Zhang, Ning Yi, Kairui Tang, Michael Dexheimer, Xiaojun Lian, Q Wang, J Yang, Jennifer Gray, Huanyu Cheng, 2021, Biosensors and Bioelectronics on p. 113606

Human pluripotent stem cell culture density modulates YAP signaling

Cheston Hsiao, Michael Lampe, Songkhun Nillasithanukroh, Wenqing Han, Xiaojun Lian, Sean P. Palecek, 2016, Biotechnology Journal on p. 662-675

News Articles Featuring Xiaojun (Lance) Lian

Dec 19, 2022

New 'Life From All Angles' video highlights Lance Lian’s stem cell research

The latest episode of "Life From All Angles," a YouTube series from the Huck Institutes of the Life Sciences, features Associate Professor of Biomedical Engineering Xiaojun "Lance" Lian and his efforts to produce new cardiomyocytes to advance the effectiveness of stem cell differentiation.

Full Article

Sep 27, 2022

Game-changing stem cell engineer Lance Lian featured on Symbiotic podcast

Xiaojun “Lance” Lian, associate professor of biomedical engineering, is set to be the Symbiotic podcast’s final guest for 2022 when he appears at noon on Wednesday, Sept. 28 on the Huck Institutes of the Life Sciences’ livestream page.

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Sep 09, 2022

Penn State-led team develops more efficient system for delivering CRISPR to stem cells

Modified mRNA (modRNA)-based CRISPR systems improve on the transfection and knockout efficiency of plasmid-based systems in human pluripotent stem cells (hPSCs), a Penn State-led team of interdisciplinary researchers has found.

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Sep 07, 2022

New approach more than doubles stem cell editing efficiency, researchers report

A Penn State-led team of interdisciplinary researchers has developed techniques to improve the efficiency of CRISPR-Cas9, the genome editing technique that earned the Nobel Prize in 2020.

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Mar 07, 2022

Studying diseases with better delivery of gene-editing tools

Using gene-editing tools with stem cells can be challenging because their delivery into cells can be inefficient, time-consuming, or expensive. A Penn State-led team of interdisciplinary researchers has developed a method that improves the lifespan and efficiency of CRISPR gene-editing tools after delivery.

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