Susan Hafenstein
Director of the Center for Structural Biology; Huck Chair of Structural Virology; Professor of Biochemistry and Molecular Biology

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W231 Millennium Science Complex
University Park, PA - suh21@psu.edu
- 814-865-5729
Research Summary
Using a structural approach to learn more about viral infectivity, tropism, evolution and pathogenicity. Developing approaches to visualize critical events that cause a break from the regular symmetry of the virus, including packaging of the genome, receptor usage, antibody interactions and uncoating of the viral genome during the final stages of infection.
Huck Graduate Students
Huck Affiliations
- Center for Infectious Disease Dynamics
- Molecular, Cellular, and Integrative Biosciences
- Cryo-Electron Microscopy Facility
- Center for Structural Oncology
- Center for Structural Biology
Links
Publication Tags
Viruses Capsid Enterovirus Neutralizing Antibodies Mutation Epitopes Cryoelectron Microscopy Antibodies Receptors Proteins Cryo Electron Microscopy Dogs Lipids Canine Parvovirus Carnivore Protoparvovirus 1 Genome Infection Immunoglobulin Fragments Ribonucleoproteins Ribonucleases Capsid Proteins Amino Acids Human Papillomavirus 16 Rna NeoplasmsMost Recent Papers
High resolution cryo EM analysis of HPV16 identifies minor structural protein L2 and describes capsid flexibility
Daniel J. Goetschius, Samantha R. Hartmann, Suriyasri Subramanian, Carol M. Bator, Neil D. Christensen, Susan L. Hafenstein, 2021, Scientific Reports
Cryo em analysis reveals inherent flexibility of authentic murine papillomavirus capsids
Samantha R. Hartmann, Daniel J. Goetschius, Jiafen Hu, Joshua J. Graff, Carol M. Bator, Neil D. Christensen, Susan L. Hafenstein, 2021, Viruses
High-resolution asymmetric structure of a Fab-virus complex reveals overlap with the receptor binding site
Daniel J. Goetschius, Samantha R. Hartmann, Lindsey J. Organtini, Heather Callaway, Kai Huang, Carol M. Bator, Robert E. Ashley, Alexander M. Makhov, James F. Conway, Colin R. Parrish, Susan L. Hafenstein, 2021, Proceedings of the National Academy of Sciences of the United States of America
Activating Sphingosine-1-phospahte signaling in endothelial cells increases myosin light chain phosphorylation to decrease endothelial permeability thereby inhibiting cancer metastasis
Yu Chi Chen, Saketh S. Dinavahi, Qilong Feng, Raghavendra Gowda, Srinivasa Ramisetti, Xinghai Xia, Kyle B. LaPenna, Venkat R. Chirasani, Sung Hyun Cho, Susan L. Hafenstein, Madhu Babu Battu, Arthur Berg, Arun K. Sharma, Tom Kirchhausen, Nikolay V. Dokholyan, Shantu Amin, Pingnian He, Gavin P. Robertson, 2021, Cancer Letters on p. 107-119
Identification of a pocket factor that is critical to Zika virus assembly
Nadia M. DiNunno, Daniel J. Goetschius, Anoop Narayanan, Sydney A. Majowicz, Ibrahim Moustafa, Carol M. Bator, Susan L. Hafenstein, Joyce Jose, 2020, Nature Communications
Cryo-EM structure of catalytic ribonucleoprotein complex RNase MRP
Anna Perederina, Di Li, Hyunwook Lee, Carol Bator, Igor Berezin, Susan L. Hafenstein, Andrey S. Krasilnikov, 2020, Nature Communications
Antibody escape by polyomavirus capsid mutation facilitates neurovirulence
Matthew D. Lauver, Daniel J. Goetschius, Colleen S. Netherby-Winslow, Katelyn N. Ayers, Ge Jin, Daniel G. Haas, Elizabeth L. Frost, Sung Hyun Cho, Carol M. Bator, Stephanie M. Bywaters, Neil D. Christensen, Susan L. Hafenstein, Aron E. Lukacher, 2020, eLife on p. 1-68
Scaffold Simplification Strategy Leads to a Novel Generation of Dual Human Immunodeficiency Virus and Enterovirus-A71 Entry Inhibitors
Belén Martínez-Gualda, Liang Sun, Olaia Martí-Marí, Sam Noppen, Rana Abdelnabi, Carol M. Bator, Ernesto Quesada, Leen Delang, Carmen Mirabelli, Hyunwook Lee, Dominique Schols, Johan Neyts, Susan Hafenstein, María José Camarasa, Federico Gago, Ana San-Félix, 2020, Journal of Medicinal Chemistry on p. 349-368
Limited intrahost diversity and background evolution accompany 40 years of canine parvovirus host adaptation and spread
Ian E.H. Voorhees, Hyunwook Lee, Andrew B. Allison, Robert Lopez-Astacio, Laura B. Goodman, Oyebola O. Oyesola, Olutayo Omobowale, Olusegun Fagbohun, Edward J. Dubovi, Susan L. Hafenstein, Edward C. Holmes, Colin R. Parrish, 2020, Journal of Virology
Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy
Suriyasri Subramanian, Anna C. Maurer, Carol M. Bator, Alexander M. Makhov, James F. Conway, Kevin B. Turner, James H. Marden, Luk H. Vandenberghe, Susan L. Hafenstein, 2019, Human Gene Therapy on p. 1449-1460
Most-Cited Papers
The Enterovirus 71 A-particle Forms a Gateway to Allow Genome Release
Kristin L. Shingler, Jennifer L. Yoder, Michael S. Carnegie, Robert E. Ashley, Alexander M. Makhov, James F. Conway, Susan Hafenstein, 2013, PLoS Pathogens
Enterovirus 71 Binding to PSGL-1 on Leukocytes: VP1-145 Acts as a Molecular Switch to Control Receptor Interaction
Yorihiro Nishimura, Hyunwook Lee, Susan Hafenstein, Chikako Kataoka, Takaji Wakita, Jeffrey M. Bergelson, Hiroyuki Shimizu, 2013, PLoS Pathogens on p. e1003511
Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype
Antonio V. Bordería, Ofer Isakov, Gonzalo Moratorio, Rasmus Henningsson, Sonia Agüera-González, Lindsey Organtini, Nina F. Gnädig, Hervé Blanc, Andrés Alcover, Susan Hafenstein, Magnus Fontes, Noam Shomron, Marco Vignuzzi, 2015, PLoS Pathogens
A strain-specific epitope of enterovirus 71 identified by cryo-electron microscopy of the complex with fab from neutralizing antibody
Hyunwook Lee, Javier O. Cifuente, Robert E. Ashley, James F. Conway, Alexander M. Makhov, Yoshio Tano, Hiroyuki Shimizu, Yorihiro Nishimura, Susan Hafenstein, 2013, Journal of Virology on p. 11363-11370
A cryo-electron microscopy study identifies the complete H16.V5 epitope and reveals global conformational changes initiated by binding of the neutralizing antibody fragment
Hyunwook Lee, Sarah A. Brendle, Stephanie M. Bywaters, Jian Guan, Robert E. Ashley, Joshua D. Yoder, Alexander M. Makhov, James F. Conway, Neil D. Christensen, Susan Hafenstein, 2015, Journal of Virology on p. 1428-1438
Structural comparison of four different antibodies interacting with human papillomavirus 16 and mechanisms of neutralization
Jian Guan, Stephanie M. Bywaters, Sarah A. Brendle, Hyunwook Lee, Robert E. Ashley, Alexander M. Makhov, James F. Conway, Neil Christensen, Susan Hafenstein, 2015, Virology on p. 253-263
The role of evolutionary intermediates in the host adaptation of canine parvovirus
Karla M. Stucker, Israel Pagan, Javier O. Cifuente, Jason T. Kaelber, Tyler D. Lillie, Susan Hafenstein, Edward C. Holmes, Colin R. Parrish, 2012, Journal of Virology on p. 1514-1521
Single mutations in the VP2 300 loop region of the three-fold spike of the carnivore parvovirus capsid can determine host range
Andrew B. Allison, Lindsey J. Organtini, Sheng Zhang, Susan L. Hafenstein, Edward C. Holmes, Colin R. Parrish, 2016, Journal of Virology on p. 753-767
The crystal structure of a coxsackievirus B3-RD variant and a refined 9-angstrom cryo-electron microscopy reconstruction of the virus complexed with decay-accelerating factor (DAF) provide a new footprint of DAF on the virus surface
Joshua D. Yoder, Javier O. Cifuente, Jieyan Pan, Jeffrey M. Bergelson, Susan Hafenstein, 2012, Journal of Virology on p. 12571-12581
Kinetic and structural analysis of coxsackievirus B3 receptor interactions and formation of the A-particle
Lindsey J. Organtini, Alexander M. Makhov, James F. Conway, Susan Hafenstein, Steven D. Carsonc, 2014, Journal of Virology on p. 5755-5765
News Articles Featuring Susan Hafenstein
Apr 06, 2022
First round of funded projects announced from the Benkovic Research Initiative
Four projects have been announced as recipients of funding from the Patricia and Stephen Benkovic Research Initiative, which supports risky, highly innovative investigation at the interface of chemistry and the life sciences.
Full Article
Jun 01, 2021
New images of canine parvovirus may help predict how virus jumps to new species
Canine parvovirus (CPV) is a highly infectious pathogen that causes severe diseases in unvaccinated dogs, including inflammation of the heart and acute gastrointestinal illness. Originating in cats, the virus is a rare example of a DNA-based virus that can jump between species, and a team of researchers’ discovery may help in predicting this and the virus’ ability to evolve, which could have implications for current vaccines used in dogs.
Full Article
Nov 19, 2020
Altered 'coat' disguises fatal brain virus from neutralizing antibodies
A genetic modification in the ‘coat’ of a brain infection-causing virus may allow it to escape antibodies, according to Penn State College of Medicine researchers.
Full Article
Nov 01, 2019
Scientists pinpoint how the deadly canine parvovirus learned to infect dogs in the 1970s
Canine parvovirus, a highly contagious and deadly virus of dogs, initially infected cats and other animals long before it acquired a few mutations and started a worldwide epidemic in the late 1970s. Now, a team led by the Baker Institute’s Dr. Colin Parrishhave worked with Dr. Susan Hafenstein’s laboratory at Penn State University to show exactly how parvovirus enters canine cells, and it’s a surprisingly wobbly interaction.
Full Article
Sep 23, 2019
Virus may jump species through 'rock-and-roll' motion with receptors
Like a janitor thumbing through a keychain to find just the right key to open a lock, the "rock-and-roll" motion of the canine parvovirus during the binding process may help explain how the virus can find the spot on a receptor to infect not just dogs, but multiple species, according to an international team of researchers.
Full Article
Aug 19, 2019
Resolution Revolution: Penn State welcomes a new era of atomic-level imaging with cryo EM facility
Using extreme cold to arrest fluid samples in motion, cryo EM allows researchers to see proteins, clusters of molecules, and viruses with astounding clarity—to the point where individual atoms may become visible.
Full Article
Jun 12, 2019
Scientists discover weakness in common cold virus
An indentation on the surface of the viruses that cause a multitude of illnesses, including the common cold, has been discovered. Scientists believe this vulnerability marks a weak spot on the viruses that antiviral medications could target to administer effective treatment for colds and polio, among other illnesses
Full Article
Jun 11, 2019
A tiny crater on viruses behind the common cold may be their Achilles’ heel
A newly discovered indentation on the surface of viruses that cause many illnesses, including the common cold, could be their Achilles’ heel — and a possible target for effective drugs.
Full Article