Squire Booker
Evan Pugh Professor of Chemistry; Professor of Biochemistry and Molecular Biology
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104 Chemistry
University Park, PA 16802 - sjb14@psu.edu
- 814-865-8793
Research Summary
Elucidating the chemical mechanisms by which enzymes containing iron-sulfur clusters catalyze chemical reactions. Most ongoing projects deal with members of the Radical S-adenosylmethionine Superfamily, a diverse group of enzymes that employ radical chemistry to catalyze transformations involved in post-transcriptional and post-translational modifications, cofactor biosynthesis, secondary metabolite biosynthesis, and enzyme activation.
Huck Affiliations
Links
Publication Tags
Enzymes S Adenosylmethionine Iron Proteins Sulfur Catalysis Methionine Methylation Cofactor Biosynthesis Molecular Cluster Cleavage Vitamin B 12 Carbon Formic Acid Aldehyde Decarbonylase Aldehydes Modification Electrons Protein Spectrum Analysis Escherichia Coli Nucleic Acids X Rays CysteineMost Recent Papers
The biosynthesis of lipoic acid
Squire Booker, 2022, FASEB Journal
Structure of a B<sub>12</sub>-dependent radical SAM enzyme in carbapenem biosynthesis
Hayley L. Knox, Erica K. Sinner, Craig A. Townsend, Amie K. Boal, Squire J. Booker, 2022, Nature on p. 343-348
Structural characterization of cobalamin-dependent radical S-adenosylmethionine methylases
Hayley L. Knox, Squire J. Booker, 2022, on p. 3-27
[FeFe]-Hydrogenase
Adrien Pagnier, Batuhan Balci, Eric M. Shepard, Hao Yang, Douglas M. Warui, Stella Impano, Squire J. Booker, Brian M. Hoffman, William E. Broderick, Joan B. Broderick, 2022, Angewandte Chemie - International Edition
Using peptide substrate analogs to characterize a radical intermediate in NosN catalysis
Bo Wang, Alexey Silakov, Squire J. Booker, 2022, on p. 469-487
Structural basis for non-radical catalysis by TsrM, a radical SAM methylase
Hayley L. Knox, Percival Yang Ting Chen, Anthony J. Blaszczyk, Arnab Mukherjee, Tyler L. Grove, Erica L. Schwalm, Bo Wang, Catherine L. Drennan, Squire J. Booker, 2021, Nature Chemical Biology on p. 485-491
Biochemical Approaches to Probe the Role of the Auxiliary Iron-Sulfur Cluster of Lipoyl Synthase from Mycobacterium Tuberculosis
Vivian Robert Jeyachandran, Jay V. Pendyala, Erin L. McCarthy, Amie K. Boal, Squire J. Booker, 2021, on p. 307-332
Structural basis for carbapenem C-alkylation by TokK, a B12-dependent radical SAM enzyme
O Knox, Sinner, C Townsend, A Boal, Squire Booker, 2021, Nature
Radical S-adenosylmethionine methylases
Danielle V. Millera, Hayley L. Knoxa, Bo Wang, Erica L. Schwalm, Anthony J. Blaszczyk, Squire J. Booker, 2020, on p. 24-69
Overview and introduction
J. Martin Bollinger, Vahe Bandarian, Squire J. Booker, 2020, on p. 1-2
Most-Cited Papers
Mechanistic diversity of radical S-adenosylmethionine (SAM)-dependent methylation
Matthew R. Bauerle, Erica L. Schwalm, Squire J. Booker, 2015, Journal of Biological Chemistry on p. 3995-4002
Evidence for only oxygenative cleavage of aldehydes to alk(a/e)nes and formate by cyanobacterial aldehyde decarbonylases
Ning Li, Wei Chen Chang, Douglas Warui, Squire J. Booker, Carsten Krebs, Joseph M. Bollinger, Jr., 2012, Biochemistry on p. 7908-7916
X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification
Peter J. Goldman, Tyler L. Grove, Lauren A. Sites, Martin I. McLaughlin, Squire J. Booker, Catherine L. Drennan, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 8519-8524
RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins
Nicholas D. Lanz, Tyler L. Grove, Camelia Baleanu Gogonea, Kyung Hoon Lee, Carsten Krebs, Squire J. Booker, 2012, Methods in enzymology on p. 125-152
Mössbauer spectroscopy of Fe/S proteins
Maria Eirini Pandelia, Nicholas D. Lanz, Squire J. Booker, Carsten Krebs, 2015, Biochimica et Biophysica Acta - Molecular Cell Research on p. 1395-1405
Auxiliary iron-sulfur cofactors in radical SAM enzymes
Nicholas D. Lanz, Squire J. Booker, 2015, Biochimica et Biophysica Acta - Molecular Cell Research on p. 1316-1334
A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases
Quin H. Christensen, Tyler L. Grove, Squire J. Booker, E. Peter Greenberg, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 13815-13820
Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase
Erin L. McCarthy, Squire J. Booker, 2017, Science on p. 373-377
Atlas of the Radical SAM Superfamily
Gemma L. Holliday, Eyal Akiva, Elaine C. Meng, Shoshana D. Brown, Sara Calhoun, Ursula Pieper, Andrej Sali, Squire J. Booker, Patricia C. Babbitt, 2018, on p. 1-71
Identification and function of auxiliary iron-sulfur clusters in radical SAM enzymes
Nicholas D. Lanz, Squire J. Booker, 2012, BBA - Protein Structure on p. 1196-1212
News Articles Featuring Squire Booker
Apr 18, 2022
Booker receives Hans Neurath Award from Protein Society
Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, Holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been honored with the Hans Neurath Award from the Protein Society, the premier international society dedicated to supporting protein research.
Full Article
Apr 06, 2022
First round of funded projects announced from the Benkovic Research Initiative
Four projects have been announced as recipients of funding from the Patricia and Stephen Benkovic Research Initiative, which supports risky, highly innovative investigation at the interface of chemistry and the life sciences.
Full Article
Feb 02, 2022
The key to a powerful antibiotic’s formation now clear
A powerful class of antibiotics called carbapenems can circumvent antibiotic resistance thanks to a particular chain of atoms in their structure. Now, a team of researchers from Penn State and Johns Hopkins University have imaged an enzyme involved in the creation of this chain to better understand how it forms — and perhaps replicate the process to improve future antibiotics.
Full Article
Oct 22, 2021
How To Modify RNA: Crucial Steps for Adding Chemical Tag To Transfer RNA Discovered
The chemical steps in an important cellular modification process that adds a chemical tag to some RNAs have been revealed in a new study. Interfering with this process in humans can lead to neuronal diseases, diabetes, and cancers. A research team, led by chemists at Penn State, has imaged a protein that facilitates this RNA modification in bacteria, allowing the researchers to reconstruct the process.
Full Article
Sep 30, 2021
Enzymes show off new moves
A 2022 annual meeting session on enzymology
Full Article
Sep 15, 2021
Modifying RNA: Crucial steps for adding chemical tag to transfer RNA revealed
The chemical steps in an important cellular modification process that adds a chemical tag to some RNAs have been revealed in a new study. Interfering with this process in humans can lead to neuronal diseases, diabetes, and cancers.
Full Article
Apr 27, 2021
Squire Booker named inaugural fellow of the American Society for Biochemistry and Molecular Biology
Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been named an inaugural fellow of the American Society for Biochemistry and Molecular Biology.
Full Article
Jan 18, 2021
Synthesis of potent antibiotic follows unusual chemical pathway
Images of a protein involved in creating a potent antibiotic reveal the unusual first steps of the antibiotic’s synthesis. The improved understanding of the chemistry behind this process, detailed in a new study led by Penn State chemists, could allow researchers to adapt this and similar compounds for use in human medicine.
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Sep 30, 2020
Mapping a trail of chemical reactions, Howard Hughes Investigator Squire Booker shows how one group of enzymes helps us stay healthy
Mapping a trail of chemical reactions, Howard Hughes Investigator Squire Booker shows how one group of enzymes helps us stay healthy
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May 01, 2019
Squire Booker Elected as Member of the National Academy of Sciences
Squire J. Booker, professor of chemistry and of biochemistry and molecular biology at Penn State, Holder of the Eberly Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been elected as a member of the National Academy of Sciences.
Full Article