Squire Booker

Evan Pugh Professor of Chemistry; Professor of Biochemistry and Molecular Biology

Squire Booker

Research Summary

Elucidating the chemical mechanisms by which enzymes containing iron-sulfur clusters catalyze chemical reactions. Most ongoing projects deal with members of the Radical S-adenosylmethionine Superfamily, a diverse group of enzymes that employ radical chemistry to catalyze transformations involved in post-transcriptional and post-translational modifications, cofactor biosynthesis, secondary metabolite biosynthesis, and enzyme activation.

Huck Affiliations

Links

Publication Tags

S Adenosylmethionine Enzymes Proteins Methylation Iron Biosynthesis Peptides Sulfur Escherichia Coli X Ray Crystallography Vitamin B 12 Methyltransferases Electrons Acids Substrates Health Cysteine Rna Catalysis Methionine Genes Quinolinic Acid Reducing Agents Pyochelin Oxidation Reduction

Most Recent Papers

First Step in Catalysis of the Radical <i>S</i>-Adenosylmethionine Methylthiotransferase MiaB Yields an Intermediate with a [3Fe-4S]<sup>0</sup>-Like Auxiliary Cluster.

Bao Zhang, A Arcinas, M Radle, Alexey Silakov, Squire Booker, Carsten Krebs, 2020, Journal of the American Chemical Society on p. 1911-1924

The Expanding Role of Methyl-Coenzyme M Reductase in the Anaerobic Functionalization of Alkanes.

David Miller, Squire Booker, 2019, Biochemistry on p. 4269-4271

An unexpected species determined by X-ray crystallography that may represent an intermediate in the reaction catalyzed by quinolinate synthase

Olga A. Esakova, Alexey Silakov, Tyler L. Grove, Douglas M. Warui, Neela H. Yennawar, Squire J. Booker, 2019, Journal of the American Chemical Society on p. 14142-14151

Understanding the role of electron donors in the reaction catalyzed by Tsrm, a cobalamin-dependent radical S-adenosylmethionine methylase

Anthony J. Blaszczyk, Hayley L. Knox, Squire J. Booker, 2019, Journal of Biological Inorganic Chemistry on p. 831-839

Methanogenesis marker protein 10 (Mmp10) from Methanosarcina acetivorans is a radical S-adenosylmethionine methylase that unexpectedly requires cobalamin

M Radle, David Miller, T Laremore, Squire Booker, 2019, The Journal of Biological Chemistry on p. 11712-11725

Analysis of RNA Methylation by Phylogenetically Diverse Cfr Radical S-Adenosylmethionine Enzymes Reveals an Iron-Binding Accessory Domain in a Clostridial Enzyme

James D. Gumkowski, Ryan J. Martinie, Patrick S. Corrigan, Juan Pan, Matthew R. Bauerle, Mohamed Almarei, Squire J. Booker, Alexey Silakov, Carsten Krebs, Amie Kathleen Boal, 2019, Biochemistry on p. 3169-3184

Capturing Intermediates in the Reaction Catalyzed by NosN, a Class C Radical S-Adenosylmethionine Methylase Involved in the Biosynthesis of the Nosiheptide Side-Ring System

Bo Wang, Joseph W. Lamattina, Savannah L. Marshall, Squire J. Booker, 2019, Journal of the American Chemical Society on p. 5788-5797

Stuffed methyltransferase catalyzes the penultimate step of pyochelin biosynthesis

Trey A. Ronnebaum, Jeffrey S. McFarlane, Thomas E. Prisinzano, Squire J. Booker, Audrey L. Lamb, 2019, Biochemistry on p. 665-678

The A-type domain in Escherichia coli NfuA is required for regenerating the auxiliary [4Fe– 4S] cluster in Escherichia coli lipoyl synthase

Erin L. McCarthy, Ananda N. Rankin, Zerick R. Dill, Squire J. Booker, 2019, Journal of Biological Chemistry on p. 1609-1617

Parsing redox potentials of five ferredoxins found within Thermotoga maritima

Stephanie J. Maiocco, Arthur J. Arcinas, Squire J. Booker, Sean J. Elliott, 2019, Protein Science on p. 257-266

Most-Cited Papers

A radically different mechanism for S-adenosylmethionine-dependent methyltransferases

Tyler L. Grove, Jack S. Benner, Matthew I. Radle, Jessica H. Ahlum, Bradley J. Landgraf, Carsten Krebs, Squire J. Booker, 2011, Science on p. 604-607

Structural basis for methyl transfer by a radical SAM enzyme

Amie Kathleen Boal, Tyler L. Grove, Monica I. McLaughlin, Neela Yennawar, Squire J. Booker, Amy C. Rosenzweig, 2011, Science on p. 1089-1092

Radical S-Adenosylmethionine Enzymes in Human Health and Disease

Bradley J. Landgraf, Erin L. McCarthy, Squire J. Booker, 2016, Annual Review of Biochemistry on p. 485-514

X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification

Peter J. Goldman, Tyler L. Grove, Lauren A. Sites, Martin I. McLaughlin, Squire J. Booker, Catherine L. Drennan, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 8519-8524

RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins

Nicholas D. Lanz, Tyler L. Grove, Camelia Baleanu Gogonea, Kyung Hoon Lee, Carsten Krebs, Squire J. Booker, 2012, Methods in enzymology on p. 125-152

Cfr and RlmN contain a single [4Fe-4S] cluster, which directs two distinct reactivities for s -adenosylmethionine: Methyl transfer by S <sub>N</sub>2 displacement and radical generation

Tyler L. Grove, Matthew I. Radle, Carsten Krebs, Squire J. Booker, 2011, Journal of the American Chemical Society on p. 19586-19589

A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases

Quin H. Christensen, Tyler L. Grove, Squire J. Booker, E. Peter Greenberg, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 13815-13820

Consecutive radical S-adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis

Daniel R. Marous, Evan P. Lloyd, Andrew R. Buller, Kristos A. Moshos, Tyler L. Grove, Anthony J. Blaszczyk, Squire J. Booker, Craig A. Townsend, 2015, Proceedings of the National Academy of Sciences of the United States of America on p. 10354-10358

X-ray analysis of butirosin biosynthetic enzyme BtrN redefines structural motifs for AdoMet radical chemistry

Peter J. Goldman, Tyler L. Groèe, Squire J. Booker, Catherine L. Drennan, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 15949-15954

Crystallographic snapshots of sulfur insertion by lipoyl synthase

Martin I. McLaughlin, Nicholas D. Lanz, Peter J. Goldman, Kyung Hoon Lee, Squire J. Booker, Catherine L. Drennan, 2016, Proceedings of the National Academy of Sciences of the United States of America on p. 9446-9450