Squire Booker

Evan Pugh Professor of Chemistry; Professor of Biochemistry and Molecular Biology

Squire Booker

Research Summary

Elucidating the chemical mechanisms by which enzymes containing iron-sulfur clusters catalyze chemical reactions. Most ongoing projects deal with members of the Radical S-adenosylmethionine Superfamily, a diverse group of enzymes that employ radical chemistry to catalyze transformations involved in post-transcriptional and post-translational modifications, cofactor biosynthesis, secondary metabolite biosynthesis, and enzyme activation.

Huck Affiliations

Links

Publication Tags

Enzymes S Adenosylmethionine Oxidation Reduction Aldehydes Proteins Formic Acid Oxygenases Methylation Electrons Substrates Aldehyde Decarbonylase Iron Biosynthesis Fatty Aldehyde Ferredoxins Catalysis Sulfur Peroxides Peptides Carbon Monoxide Escherichia Coli X Ray Crystallography Methyltransferases Oxygen Acids

Most Recent Papers

First step in catalysis of the radical s-adenosylmethionine methylthiotransferase MiaB yields an intermediate with a [3Fe-4S]0-like auxiliary cluster

Bo Zhang, Arthur J. Arcinas, Matthew I. Radle, Alexey Silakov, Squire J. Booker, Carsten Krebs, 2020, Journal of the American Chemical Society on p. 1911-1924

The Expanding Role of Methyl-Coenzyme M Reductase in the Anaerobic Functionalization of Alkanes.

D Miller, Squire Booker, 2019, Biochemistry on p. 4269-4271

An unexpected species determined by X-ray crystallography that may represent an intermediate in the reaction catalyzed by quinolinate synthase

Olga A. Esakova, Alexey Silakov, Tyler L. Grove, Douglas M. Warui, Neela H. Yennawar, Squire J. Booker, 2019, Journal of the American Chemical Society on p. 14142-14151

Understanding the role of electron donors in the reaction catalyzed by Tsrm, a cobalamin-dependent radical S-adenosylmethionine methylase

Anthony J. Blaszczyk, Hayley L. Knox, Squire J. Booker, 2019, Journal of Biological Inorganic Chemistry on p. 831-839

Methanogenesis marker protein 10 (Mmp10) from Methanosarcina acetivorans is a radical S-adenosylmethionine methylase that unexpectedly requires cobalamin

M Radle, D Miller, T Laremore, Squire Booker, 2019, The Journal of Biological Chemistry on p. 11712-11725

Analysis of RNA Methylation by Phylogenetically Diverse Cfr Radical S-Adenosylmethionine Enzymes Reveals an Iron-Binding Accessory Domain in a Clostridial Enzyme

James D. Gumkowski, Ryan J. Martinie, Patrick S. Corrigan, Juan Pan, Matthew R. Bauerle, Mohamed Almarei, Squire J. Booker, Alexey Silakov, Carsten Krebs, Amie Kathleen Boal, 2019, Biochemistry on p. 3169-3184

Capturing Intermediates in the Reaction Catalyzed by NosN, a Class C Radical S-Adenosylmethionine Methylase Involved in the Biosynthesis of the Nosiheptide Side-Ring System

Bo Wang, Joseph W. Lamattina, Savannah L. Marshall, Squire J. Booker, 2019, Journal of the American Chemical Society on p. 5788-5797

Stuffed methyltransferase catalyzes the penultimate step of pyochelin biosynthesis

Trey A. Ronnebaum, Jeffrey S. McFarlane, Thomas E. Prisinzano, Squire J. Booker, Audrey L. Lamb, 2019, Biochemistry on p. 665-678

Parsing redox potentials of five ferredoxins found within Thermotoga maritima

Stephanie J. Maiocco, Arthur J. Arcinas, Squire J. Booker, Sean J. Elliott, 2019, Protein Science on p. 257-266

Ferredoxins as interchangeable redox components in support of MiaB, a radical S-adenosylmethionine methylthiotransferase

Arthur J. Arcinas, Stephanie J. Maiocco, Sean J. Elliott, Alexey Silakov, Squire J. Booker, 2019, Protein Science on p. 267-282

Most-Cited Papers

A radically different mechanism for S-adenosylmethionine-dependent methyltransferases

Tyler L. Grove, Jack S. Benner, Matthew I. Radle, Jessica H. Ahlum, Bradley J. Landgraf, Carsten Krebs, Squire J. Booker, 2011, Science on p. 604-607

Structural basis for methyl transfer by a radical SAM enzyme

Amie Kathleen Boal, Tyler L. Grove, Monica I. McLaughlin, Neela Yennawar, Squire J. Booker, Amy C. Rosenzweig, 2011, Science on p. 1089-1092

Radical S-Adenosylmethionine Enzymes in Human Health and Disease

Bradley J. Landgraf, Erin L. McCarthy, Squire J. Booker, 2016, Annual Review of Biochemistry on p. 485-514

Evidence for only oxygenative cleavage of aldehydes to alk(a/e)nes and formate by cyanobacterial aldehyde decarbonylases

Ning Li, Wei Chen Chang, Douglas Warui, Squire J. Booker, Carsten Krebs, Joseph M. Bollinger, Jr., 2012, Biochemistry on p. 7908-7916

Detection of formate, rather than carbon monoxide, as the stoichiometric coproduct in conversion of fatty aldehydes to alkanes by a cyanobacterial aldehyde decarbonylase

Douglas M. Warui, Ning Li, Hanne Nørgaard, Carsten Krebs, J. Martin Bollinger, Squire J. Booker, 2011, Journal of the American Chemical Society on p. 3316-3319

Conversion of fatty aldehydes to alka(e)nes and formate by a cyanobacterial aldehyde decarbonylase: Cryptic redox by an unusual dimetal oxygenase

Ning Li, Hanne Nørgaard, Douglas M. Warui, Squire J. Booker, Carsten Krebs, J. Martin Bollinger, 2011, Journal of the American Chemical Society on p. 6158-6161

X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification

Peter J. Goldman, Tyler L. Grove, Lauren A. Sites, Martin I. McLaughlin, Squire J. Booker, Catherine L. Drennan, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 8519-8524

A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases

Quin H. Christensen, Tyler L. Grove, Squire J. Booker, E. Peter Greenberg, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 13815-13820

RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins

Nicholas D. Lanz, Tyler L. Grove, Camelia Baleanu Gogonea, Kyung Hoon Lee, Carsten Krebs, Squire J. Booker, 2012, Methods in enzymology on p. 125-152

Substrate-triggered addition of dioxygen to the diferrous cofactor of aldehyde-deformylating oxygenase to form a diferric-peroxide intermediate

Maria E. Pandelia, Ning Li, Hanne Nørgaard, Douglas Warui, Lauren J. Rajakovich, Wei Chen Chang, Squire J. Booker, Carsten Krebs, Joseph M. Bollinger, Jr., 2013, Journal of the American Chemical Society on p. 15801-15812