Evan Pugh Professor of Chemistry; Professor of Biochemistry and Molecular Biology
Elucidating the chemical mechanisms by which enzymes containing iron-sulfur clusters catalyze chemical reactions. Most ongoing projects deal with members of the Radical S-adenosylmethionine Superfamily, a diverse group of enzymes that employ radical chemistry to catalyze transformations involved in post-transcriptional and post-translational modifications, cofactor biosynthesis, secondary metabolite biosynthesis, and enzyme activation.
Publication TagsEnzymes Proteins Sulfur S Adenosylmethionine Iron Molecular Cluster Cofactor Biosynthesis Catalysis Protein Methionine Electrons Cleavage Escherichia Coli Aldehydes Formic Acid Aldehyde Decarbonylase Modification Ferredoxins In Vitro Techniques Thioctic Acid Lipoic Acid Sulfur Atom Spectrum Analysis Nucleic Acids
Most Recent Publications
Characterization of LipS1 and LipS2 from Thermococcus kodakarensis: Proteins Annotated as Biotin Synthases, which Together Catalyze Formation of the Lipoyl Cofactor
Syam Sundar Neti, Debangsu Sil, Douglas M. Warui, Olga A. Esakova, Amy E. Solinski, Dante A. Serrano, Carsten Krebs, Squire J. Booker, 2022, ACS Bio and Med Chem Au on p. 509-520
Douglas M. Warui, Debangsu Sil, Kyung Hoon Lee, Syam Sundar Neti, Olga A. Esakova, Hayley L. Knox, Carsten Krebs, Squire J. Booker, 2022, ACS Bio and Med Chem Au on p. 456-468
Cody T. Lloyd, David F. Iwig, Bo Wang, Matteo Cossu, William W. Metcalf, Amie K. Boal, Squire J. Booker, 2022, Nature on p. 197-203
[FeFe]-Hydrogenase: Defined Lysate-Free Maturation Reveals a Key Role for Lipoyl-H-Protein in DTMA Ligand Biosynthesis
Adrien Pagnier, Batuhan Balci, Eric M. Shepard, Hao Yang, Douglas M. Warui, Stella Impano, Squire J. Booker, Brian M. Hoffman, William E. Broderick, Joan B. Broderick, 2022, Angewandte Chemie - International Edition
The biosynthesis of lipoic acid
Squire Booker, 2022, FASEB Journal
Happy Birthday ACS Bio & Med Chem Au!
Squire J. Booker, 2022, ACS Bio and Med Chem Au on p. 1-3
Hayley L. Knox, Erica K. Sinner, Craig A. Townsend, Amie K. Boal, Squire J. Booker, 2022, Nature on p. 343-348
Structural characterization of cobalamin-dependent radical S-adenosylmethionine methylases
Hayley L. Knox, Squire J. Booker, 2022, on p. 3-27
Using peptide substrate analogs to characterize a radical intermediate in NosN catalysis
Bo Wang, Alexey Silakov, Squire J. Booker, 2022, on p. 469-487
Vinzent Schulz, Somsuvro Basu, Sven A. Freibert, Holger Webert, Linda Boss, Ulrich Mühlenhoff, Fabien Pierrel, Lars O. Essen, Douglas M. Warui, Squire J. Booker, Oliver Stehling, Roland Lill, 2022, Nature Chemical Biology
Radical S-Adenosylmethionine Enzymes in Human Health and Disease
Bradley Landgraf, Erin McCarthy, Squire Booker, 2016, Annual Review of Biochemistry on p. 485-514
Evidence for only oxygenative cleavage of aldehydes to alk(a/e)nes and formate by cyanobacterial aldehyde decarbonylases
Ning Li, Wei Chen Chang, Douglas Warui, Squire J. Booker, Carsten Krebs, Joseph M. Bollinger, Jr., 2012, Biochemistry on p. 7908-7916
X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification
Peter J. Goldman, Tyler L. Grove, Lauren A. Sites, Martin I. McLaughlin, Squire J. Booker, Catherine L. Drennan, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 8519-8524
RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins
Nicholas D. Lanz, Tyler L. Grove, Camelia Baleanu Gogonea, Kyung Hoon Lee, Carsten Krebs, Squire J. Booker, 2012, Methods in enzymology on p. 125-152
Maria-Eirini Pandelia, Nicholas Lanz, Squire Booker, Carsten Krebs, 2015, Biochimica et Biophysica Acta - Molecular Cell Research on p. 1395-1405
Atlas of the Radical SAM Superfamily: Divergent Evolution of Function Using a “Plug and Play” Domain
Gemma L. Holliday, Eyal Akiva, Elaine C. Meng, Shoshana D. Brown, Sara Calhoun, Ursula Pieper, Andrej Sali, Squire J. Booker, Patricia C. Babbitt, 2018, on p. 1-71
Auxiliary iron-sulfur cofactors in radical SAM enzymes
Nicholas Lanz, Squire Booker, 2015, Biochimica et Biophysica Acta - Molecular Cell Research on p. 1316-1334
Erin L. McCarthy, Squire J. Booker, 2017, Science on p. 373-377
A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases
Quin H. Christensen, Tyler L. Grove, Squire J. Booker, E. Peter Greenberg, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 13815-13820
Identification and function of auxiliary iron-sulfur clusters in radical SAM enzymes
Nicholas D. Lanz, Squire J. Booker, 2012, BBA - Protein Structure on p. 1196-1212
News Articles Featuring Squire Booker
Aug 22, 2022
Revealed missing step in lipid formation could enable detection of past climate
The missing step in the formation of a lipid molecule that allows certain single-celled organisms to survive the most extreme environments on Earth has now been deciphered.
Aug 18, 2022
Booker honored by American Society for Biochemistry and Molecular Biology
Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been awarded the ASBMB-Merck Award and the Ruth Kirschstein Diversity in Science Award from the American Society of Biochemistry and Molecular Biology (ASBMB)
Apr 18, 2022
Booker receives Hans Neurath Award from Protein Society
Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, Holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been honored with the Hans Neurath Award from the Protein Society, the premier international society dedicated to supporting protein research.
Apr 06, 2022
First round of funded projects announced from the Benkovic Research Initiative
Four projects have been announced as recipients of funding from the Patricia and Stephen Benkovic Research Initiative, which supports risky, highly innovative investigation at the interface of chemistry and the life sciences.
Feb 02, 2022
The key to a powerful antibiotic’s formation now clear
A powerful class of antibiotics called carbapenems can circumvent antibiotic resistance thanks to a particular chain of atoms in their structure. Now, a team of researchers from Penn State and Johns Hopkins University have imaged an enzyme involved in the creation of this chain to better understand how it forms — and perhaps replicate the process to improve future antibiotics.
Oct 22, 2021
How To Modify RNA: Crucial Steps for Adding Chemical Tag To Transfer RNA Discovered
The chemical steps in an important cellular modification process that adds a chemical tag to some RNAs have been revealed in a new study. Interfering with this process in humans can lead to neuronal diseases, diabetes, and cancers. A research team, led by chemists at Penn State, has imaged a protein that facilitates this RNA modification in bacteria, allowing the researchers to reconstruct the process.
Sep 30, 2021
Enzymes show off new moves
A 2022 annual meeting session on enzymology
Sep 15, 2021
Modifying RNA: Crucial steps for adding chemical tag to transfer RNA revealed
The chemical steps in an important cellular modification process that adds a chemical tag to some RNAs have been revealed in a new study. Interfering with this process in humans can lead to neuronal diseases, diabetes, and cancers.
Apr 27, 2021
Squire Booker named inaugural fellow of the American Society for Biochemistry and Molecular Biology
Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been named an inaugural fellow of the American Society for Biochemistry and Molecular Biology.
Jan 18, 2021
Synthesis of potent antibiotic follows unusual chemical pathway
Images of a protein involved in creating a potent antibiotic reveal the unusual first steps of the antibiotic’s synthesis. The improved understanding of the chemistry behind this process, detailed in a new study led by Penn State chemists, could allow researchers to adapt this and similar compounds for use in human medicine.