Squire Booker

Evan Pugh Professor of Chemistry; Professor of Biochemistry and Molecular Biology

Squire Booker

Research Summary

Elucidating the chemical mechanisms by which enzymes containing iron-sulfur clusters catalyze chemical reactions. Most ongoing projects deal with members of the Radical S-adenosylmethionine Superfamily, a diverse group of enzymes that employ radical chemistry to catalyze transformations involved in post-transcriptional and post-translational modifications, cofactor biosynthesis, secondary metabolite biosynthesis, and enzyme activation.

Huck Affiliations

Links

Publication Tags

Enzymes Sulfur Iron Catalysis S Adenosylmethionine Proteins Aldehydes Molecular Cluster Formic Acid Substrates Methionine Oxygenases Methylation X Ray Crystallography Peroxides Electrons Oxygen Atoms Quinolinic Acid Formate Iminoaspartic Acid Aldehyde Decarbonylase Carbon Dihydroxyacetone Phosphate Molecules

Most Recent Papers

Structural basis for tRNA methylthiolation by the radical SAM enzyme MiaB

Olga A. Esakova, Tyler L. Grove, Neela H. Yennawar, Arthur J. Arcinas, Bo Wang, Carsten Krebs, Steven C. Almo, Squire J. Booker, 2021, Nature on p. 566-570

Structural basis for non-radical catalysis by TsrM, a radical SAM methylase

Hayley L. Knox, Percival Yang Ting Chen, Anthony J. Blaszczyk, Arnab Mukherjee, Tyler L. Grove, Erica L. Schwalm, Bo Wang, Catherine L. Drennan, Squire J. Booker, 2021, Nature Chemical Biology on p. 485-491

Biochemical Approaches to Probe the Role of the Auxiliary Iron-Sulfur Cluster of Lipoyl Synthase from Mycobacterium Tuberculosis

Vivian Robert Jeyachandran, Jay V. Pendyala, Erin L. McCarthy, Amie K. Boal, Squire J. Booker, 2021, on p. 307-332

Radical S-adenosylmethionine methylases

Danielle V. Millera, Hayley L. Knoxa, Bo Wang, Erica L. Schwalm, Anthony J. Blaszczyk, Squire J. Booker, 2020, on p. 24-69

Overview and introduction

J. Martin Bollinger, Vahe Bandarian, Squire J. Booker, 2020, on p. 1-2

First step in catalysis of the radical s-adenosylmethionine methylthiotransferase MiaB yields an intermediate with a [3Fe-4S]0-like auxiliary cluster

Bo Zhang, Arthur J. Arcinas, Matthew I. Radle, Alexey Silakov, Squire J. Booker, Carsten Krebs, 2020, Journal of the American Chemical Society on p. 1911-1924

The biosynthesis of lipoic acid

E. McCarthy, Squire Booker, 2020, Comprehensive Natural Products III. Chemistry and Biology

Crystallographic snapshots of TsrM, a radical S-adenosylmethionine enzyme whose reaction is not so radical

Hayley Knox, Percival Chen, Anthony Blaszczyk, Tyler Grove, Erica Schwalm, B Wang, Catherine Drennan, Squire Booker, 2020, FASEB JOURNAL

The expanding role of methyl-coenzyme m reductase in the anaerobic functionalization of alkanes

Danielle V. Miller, Squire J. Booker, 2019, Biochemistry on p. 4269-4271

An unexpected species determined by X-ray crystallography that may represent an intermediate in the reaction catalyzed by quinolinate synthase

Olga A. Esakova, Alexey Silakov, Tyler L. Grove, Douglas M. Warui, Neela H. Yennawar, Squire J. Booker, 2019, Journal of the American Chemical Society on p. 14142-14151

Most-Cited Papers

Mechanistic diversity of radical S-adenosylmethionine (SAM)-dependent methylation

Matthew R. Bauerle, Erica L. Schwalm, Squire J. Booker, 2015, Journal of Biological Chemistry on p. 3995-4002

Evidence for only oxygenative cleavage of aldehydes to alk(a/e)nes and formate by cyanobacterial aldehyde decarbonylases

Ning Li, Wei Chen Chang, Douglas Warui, Squire J. Booker, Carsten Krebs, Joseph M. Bollinger, Jr., 2012, Biochemistry on p. 7908-7916

X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification

Peter J. Goldman, Tyler L. Grove, Lauren A. Sites, Martin I. McLaughlin, Squire J. Booker, Catherine L. Drennan, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 8519-8524

RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins

Nicholas D. Lanz, Tyler L. Grove, Camelia Baleanu Gogonea, Kyung Hoon Lee, Carsten Krebs, Squire J. Booker, 2012, Methods in enzymology on p. 125-152

Mössbauer spectroscopy of Fe/S proteins

Maria Eirini Pandelia, Nicholas D. Lanz, Squire J. Booker, Carsten Krebs, 2015, Biochimica et Biophysica Acta - Molecular Cell Research on p. 1395-1405

A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases

Quin H. Christensen, Tyler L. Grove, Squire J. Booker, E. Peter Greenberg, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 13815-13820

Auxiliary iron-sulfur cofactors in radical SAM enzymes

Nicholas D. Lanz, Squire J. Booker, 2015, Biochimica et Biophysica Acta - Molecular Cell Research on p. 1316-1334

Identification and function of auxiliary iron-sulfur clusters in radical SAM enzymes

Nicholas D. Lanz, Squire J. Booker, 2012, BBA - Protein Structure on p. 1196-1212

Substrate-triggered addition of dioxygen to the diferrous cofactor of aldehyde-deformylating oxygenase to form a diferric-peroxide intermediate

Maria E. Pandelia, Ning Li, Hanne Nørgaard, Douglas M. Warui, Lauren J. Rajakovich, Wei Chen Chang, Squire J. Booker, Carsten Krebs, J. Martin Bollinger, 2013, Journal of the American Chemical Society on p. 15801-15812

Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase

Erin L. McCarthy, Squire J. Booker, 2017, Science on p. 373-377

News Articles Featuring Squire Booker

How To Modify RNA: Crucial Steps for Adding Chemical Tag To Transfer RNA Discovered

The chemical steps in an important cellular modification process that adds a chemical tag to some RNAs have been revealed in a new study. Interfering with this process in humans can lead to neuronal diseases, diabetes, and cancers. A research team, led by chemists at Penn State, has imaged a protein that facilitates this RNA modification in bacteria, allowing the researchers to reconstruct the process.

Enzymes show off new moves

A 2022 annual meeting session on enzymology

Modifying RNA: Crucial steps for adding chemical tag to transfer RNA revealed

The chemical steps in an important cellular modification process that adds a chemical tag to some RNAs have been revealed in a new study. Interfering with this process in humans can lead to neuronal diseases, diabetes, and cancers.

Squire Booker named inaugural fellow of the American Society for Biochemistry and Molecular Biology

Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been named an inaugural fellow of the American Society for Biochemistry and Molecular Biology.

Synthesis of potent antibiotic follows unusual chemical pathway

Images of a protein involved in creating a potent antibiotic reveal the unusual first steps of the antibiotic’s synthesis. The improved understanding of the chemistry behind this process, detailed in a new study led by Penn State chemists, could allow researchers to adapt this and similar compounds for use in human medicine.

Mapping a trail of chemical reactions, Howard Hughes Investigator Squire Booker shows how one group of enzymes helps us stay healthy

Mapping a trail of chemical reactions, Howard Hughes Investigator Squire Booker shows how one group of enzymes helps us stay healthy

Squire Booker Elected as Member of the National Academy of Sciences

Squire J. Booker, professor of chemistry and of biochemistry and molecular biology at Penn State, Holder of the Eberly Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been elected as a member of the National Academy of Sciences.