Squire Booker
Evan Pugh Professor of Chemistry; Professor of Biochemistry and Molecular Biology

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104 Chemistry
University Park, PA 16802 - sjb14@psu.edu
- 814-865-8793
Research Summary
Elucidating the chemical mechanisms by which enzymes containing iron-sulfur clusters catalyze chemical reactions. Most ongoing projects deal with members of the Radical S-adenosylmethionine Superfamily, a diverse group of enzymes that employ radical chemistry to catalyze transformations involved in post-transcriptional and post-translational modifications, cofactor biosynthesis, secondary metabolite biosynthesis, and enzyme activation.
Huck Affiliations
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Publication Tags
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Enzymes S Adenosylmethionine Sulfur Proteins Cofactor Biosynthesis Methylation Iron Protein Carbon Vitamin B 12 Molecular Cluster Catalysis Carbon Atom Carbapenems Nitrogen Thioctic Acid Chemistry Lipoic Acid Methionine Hydrogenase Ligands X Rays Nucleic Acids LigandMost Recent Publications
FDX1 regulates cellular protein lipoylation through direct binding to LIAS
Margaret B. Dreishpoon, Nolan R. Bick, Boryana Petrova, Douglas M. Warui, Alison Cameron, Squire J. Booker, Naama Kanarek, Todd R. Golub, Peter Tsvetkov, 2023, Journal of Biological Chemistry
Enzymatic Fluoromethylation Enabled by the S-Adenosylmethionine Analog Te-Adenosyl- L-(fluoromethyl)homotellurocysteine
Syam Sundar Neti, Bo Wang, David F. Iwig, Elizabeth L. Onderko, Squire J. Booker, 2023, ACS Central Science on p. 905-914
Twenty Years of Radical SAM! the Genesis of the Superfamily
Squire J. Booker, Cody T. Lloyd, 2022, ACS Bio and Med Chem Au on p. 538-547
Discovery, structure and mechanism of a tetraether lipid synthase
Cody T. Lloyd, David F. Iwig, Bo Wang, Matteo Cossu, William W. Metcalf, Amie K. Boal, Squire J. Booker, 2022, Nature on p. 197-203
Characterization of LipS1 and LipS2 from Thermococcus kodakarensis: Proteins Annotated as Biotin Synthases, which Together Catalyze Formation of the Lipoyl Cofactor
Syam Sundar Neti, Debangsu Sil, Douglas M. Warui, Olga A. Esakova, Amy E. Solinski, Dante A. Serrano, Carsten Krebs, Squire J. Booker, 2022, ACS Bio and Med Chem Au on p. 509-520
The biosynthesis of lipoic acid
Squire Booker, 2022, FASEB Journal
[FeFe]-Hydrogenase: Defined Lysate-Free Maturation Reveals a Key Role for Lipoyl-H-Protein in DTMA Ligand Biosynthesis
Adrien Pagnier, Batuhan Balci, Eric M. Shepard, Hao Yang, Douglas M. Warui, Stella Impano, Squire J. Booker, Brian M. Hoffman, William E. Broderick, Joan B. Broderick, 2022, Angewandte Chemie - International Edition
Happy Birthday ACS Bio & Med Chem Au!
Squire J. Booker, 2022, ACS Bio and Med Chem Au on p. 1-3
Structure of a B<sub>12</sub>-dependent radical SAM enzyme in carbapenem biosynthesis
Hayley L. Knox, Erica K. Sinner, Craig A. Townsend, Amie K. Boal, Squire J. Booker, 2022, Nature on p. 343-348
Structural characterization of cobalamin-dependent radical S-adenosylmethionine methylases
Hayley L. Knox, Squire J. Booker, 2022, on p. 3-27
Most-Cited Papers
Mechanistic diversity of radical S-adenosylmethionine (SAM)-dependent methylation
Matthew Bauerle, Erica Schwalm, Squire Booker, 2015, Journal of Biological Chemistry on p. 3995-4002
Radical S-Adenosylmethionine Enzymes in Human Health and Disease
Bradley Landgraf, Erin McCarthy, Squire Booker, 2016, Annual Review of Biochemistry on p. 485-514
X-ray structure of an AdoMet radical activase reveals an anaerobic solution for formylglycine posttranslational modification
Peter J. Goldman, Tyler L. Grove, Lauren A. Sites, Martin I. McLaughlin, Squire J. Booker, Catherine L. Drennan, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 8519-8524
Atlas of the Radical SAM Superfamily: Divergent Evolution of Function Using a “Plug and Play” Domain
Gemma L. Holliday, Eyal Akiva, Elaine C. Meng, Shoshana D. Brown, Sara Calhoun, Ursula Pieper, Andrej Sali, Squire J. Booker, Patricia C. Babbitt, 2018, on p. 1-71
Mössbauer spectroscopy of Fe/S proteins
Maria-Eirini Pandelia, Nicholas Lanz, Squire Booker, Carsten Krebs, 2015, Biochimica et Biophysica Acta - Molecular Cell Research on p. 1395-1405
Auxiliary iron-sulfur cofactors in radical SAM enzymes
Nicholas Lanz, Squire Booker, 2015, Biochimica et Biophysica Acta - Molecular Cell Research on p. 1316-1334
Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase
Erin L. McCarthy, Squire J. Booker, 2017, Science on p. 373-377
A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases
Quin H. Christensen, Tyler L. Grove, Squire J. Booker, E. Peter Greenberg, 2013, Proceedings of the National Academy of Sciences of the United States of America on p. 13815-13820
Crystallographic snapshots of sulfur insertion by lipoyl synthase
Martin I. McLaughlin, Nicholas D. Lanz, Peter J. Goldman, Kyung Hoon Lee, Squire J. Booker, Catherine L. Drennan, 2016, Proceedings of the National Academy of Sciences of the United States of America on p. 9446-9450
Consecutive radical S-adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis
Daniel R. Marous, Evan P. Lloyd, Andrew R. Buller, Kristos A. Moshos, Tyler L. Grove, Anthony J. Blaszczyk, Squire J. Booker, Craig A. Townsend, 2015, Proceedings of the National Academy of Sciences of the United States of America on p. 10354-10358
News Articles Featuring Squire Booker
Dec 05, 2022
Why synonymous mutations are not always silent
New modeling shows how synonymous mutations — those that change the DNA sequence of a gene but not the sequence of the encoded protein — can still impact protein production and function.
Full Article
Aug 22, 2022
Revealed missing step in lipid formation could enable detection of past climate
The missing step in the formation of a lipid molecule that allows certain single-celled organisms to survive the most extreme environments on Earth has now been deciphered.
Full Article
Aug 18, 2022
Booker honored by American Society for Biochemistry and Molecular Biology
Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been awarded the ASBMB-Merck Award and the Ruth Kirschstein Diversity in Science Award from the American Society of Biochemistry and Molecular Biology (ASBMB)
Full Article
Apr 18, 2022
Booker receives Hans Neurath Award from Protein Society
Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, Holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been honored with the Hans Neurath Award from the Protein Society, the premier international society dedicated to supporting protein research.
Full Article
Apr 06, 2022
First round of funded projects announced from the Benkovic Research Initiative
Four projects have been announced as recipients of funding from the Patricia and Stephen Benkovic Research Initiative, which supports risky, highly innovative investigation at the interface of chemistry and the life sciences.
Full Article
Feb 02, 2022
The key to a powerful antibiotic’s formation now clear
A powerful class of antibiotics called carbapenems can circumvent antibiotic resistance thanks to a particular chain of atoms in their structure. Now, a team of researchers from Penn State and Johns Hopkins University have imaged an enzyme involved in the creation of this chain to better understand how it forms — and perhaps replicate the process to improve future antibiotics.
Full Article
Oct 22, 2021
How To Modify RNA: Crucial Steps for Adding Chemical Tag To Transfer RNA Discovered
The chemical steps in an important cellular modification process that adds a chemical tag to some RNAs have been revealed in a new study. Interfering with this process in humans can lead to neuronal diseases, diabetes, and cancers. A research team, led by chemists at Penn State, has imaged a protein that facilitates this RNA modification in bacteria, allowing the researchers to reconstruct the process.
Full Article
Sep 30, 2021
Enzymes show off new moves
A 2022 annual meeting session on enzymology
Full Article
Sep 15, 2021
Modifying RNA: Crucial steps for adding chemical tag to transfer RNA revealed
The chemical steps in an important cellular modification process that adds a chemical tag to some RNAs have been revealed in a new study. Interfering with this process in humans can lead to neuronal diseases, diabetes, and cancers.
Full Article
Apr 27, 2021
Squire Booker named inaugural fellow of the American Society for Biochemistry and Molecular Biology
Squire J. Booker, Evan Pugh Professor of Chemistry and of Biochemistry and Molecular Biology at Penn State, holder of the Eberly Family Distinguished Chair in Science, and investigator with the Howard Hughes Medical Institute, has been named an inaugural fellow of the American Society for Biochemistry and Molecular Biology.
Full Article