Howard Salis
Associate Professor of Chemical Engineering; Associate Professor of Agricultural and Biological Engineering
-
224 Ag Engineering
University Park, PA - hms17@psu.edu
- 814-863-1931
Research Summary
Engineering microorganisms for applications in synthetic biology and metabolic engineering.
Huck Affiliations
- Bioinformatics and Genomics
- Molecular, Cellular, and Integrative Biosciences
- Center for Biorenewables
Links
Publication Tags
Proteins Kinetics Rna Genes Messenger Rna Dna Bacteria Ribosomes Methane Biofuels Machine Learning Gene Expression Genome Enzymes Riboswitch Liquids Binding Sites Biosynthesis Plasmids Rna Folding Synthetic Biology Escherichia Coli Engineering Networks (Circuits) Nucleic Acid Repetitive SequencesMost Recent Papers
Automated design of thousands of nonrepetitive parts for engineering stable genetic systems
Ayaan Hossain, Eriberto Lopez, Sean M. Halper, Daniel P. Cetnar, Alexander C. Reis, Devin Strickland, Eric Klavins, Howard M. Salis, 2020, Nature Biotechnology on p. 1466-1475
Synthesis Success Calculator
Sean M. Halper, Ayaan Hossain, Howard M. Salis, 2020, ACS Synthetic Biology on p. 1563-1571
RNA size and 3-dimensional structure determine ultrafiltration behavior of small RNA molecules
Ivan Manzano, Grace Vezeau, Howard Salis, Andrew L. Zydney, 2020, Separation and Purification Technology
Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays
Alexander C. Reis, Sean M. Halper, Grace E. Vezeau, Daniel P. Cetnar, Ayaan Hossain, Phillip R. Clauer, Howard M. Salis, 2019, Nature Biotechnology on p. 1294-1301
Controlling Heterogeneity and Increasing Titer from Riboswitch-Regulated Bacillus subtilis Spores for Time-Delayed Protein Expression Applications
Denis Tamiev, Alyssa Lantz, Grace Vezeau, Howard Salis, Nigel F. Reuel, 2019, ACS Synthetic Biology on p. 2336-2346
A Canonical Biophysical Model of the CsrA Global Regulator Suggests Flexible Regulator-Target Interactions
A. N. Leistra, G. Gelderman, S. W. Sowa, A. Moon-Walker, Howard M. Salis, L. M. Contreras, 2018, Scientific Reports
Precise quantification of translation inhibition by mRNA structures that overlap with the ribosomal footprint in N-terminal coding sequences
Amin Espah Borujeni, Daniel Cetnar, Iman Farasat, Ashlee Smith, Natasha Lundgren, Howard M. Salis, 2017, Nucleic Acids Research on p. 5437-5448
Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism
Amin Espah Borujeni, Howard M. Salis, 2016, Journal of the American Chemical Society on p. 7016-7023
Reversing methanogenesis to capture methane for liquid biofuel precursors
Valerie W.C. Soo, Michael J. McAnulty, Arti Tripathi, Fayin Zhu, Limin Zhang, Emmanuel Hatzakis, Philip B. Smith, Saumya Agrawal, Hadi Nazem-Bokaee, Saratram Gopalakrishnan, Howard M. Salis, James G. Ferry, Costas D. Maranas, Andrew D. Patterson, Thomas K. Wood, 2016, Microbial Cell Factories on p. 11
Automated physics-based design of synthetic riboswitches from diverse RNA aptamers
Amin Espah Borujeni, Dennis M. Mishler, Jingzhi Wang, Walker Huso, Howard M. Salis, 2016, Nucleic Acids Research on p. 1-13
Most-Cited Papers
Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites
Amin Espah Borujeni, Anirudh S. Channarasappa, Howard M. Salis, 2014, Nucleic Acids Research on p. 2646-2659
The ribosome binding site calculator
Howard M. Salis, 2011, Methods in enzymology on p. 19-42
Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria
Iman Farasat, Manish Kushwaha, Jason Collens, Michael Easterbrook, Matthew Guido, Howard M. Salis, 2014, Molecular Systems Biology
Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration
Chiam Yu Ng, Iman Farasat, Costas D. Maranas, Howard M. Salis, 2015, Metabolic Engineering on p. 86-96
Automated physics-based design of synthetic riboswitches from diverse RNA aptamers
Amin Espah Borujeni, Dennis M. Mishler, Jingzhi Wang, Walker Huso, Howard M. Salis, 2016, Nucleic Acids Research on p. 1-13
A portable expression resource for engineering cross-species genetic circuits and pathways
Manish Kushwaha, Howard M. Salis, 2015, Nature Communications
Reversing methanogenesis to capture methane for liquid biofuel precursors
Valerie W.C. Soo, Michael J. McAnulty, Arti Tripathi, Fayin Zhu, Limin Zhang, Emmanuel Hatzakis, Philip B. Smith, Saumya Agrawal, Hadi Nazem-Bokaee, Saratram Gopalakrishnan, Howard M. Salis, James G. Ferry, Costas D. Maranas, Andrew D. Patterson, Thomas K. Wood, 2016, Microbial Cell Factories on p. 11
A predictive biophysical model of translationalcoupling to coordinate and control protein expressionin bacterial operons
Tian Tian, Howard M. Salis, 2015, Nucleic Acids Research on p. 7137-7151
Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism
Amin Espah Borujeni, Howard M. Salis, 2016, Journal of the American Chemical Society on p. 7016-7023
A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation
Iman Farasat, Howard Salis, 2016, PLoS Computational Biology on p. e1004724
News Articles Featuring Howard Salis
Jul 21, 2020
Researchers offer solution for one of synthetic biology's biggest problems
A team of Penn State and University of Washington researchers recently developed a new algorithm to help prevent the failure of engineered genetic systems, or organisms engineered to have new capabilities.
Full Article