Edward O’Brien

Associate Professor of Chemistry

Edward O’Brien

Research Summary

Developing and applying Physical Bioinformatic techniques to measure rates of translation transcriptome-wide and their molecular origins as relates to fundamental biology and disease.

Huck Graduate Students

Huck Affiliations

Publication Tags

Proteins Protein Folding Protein Ribosomes Messenger Rna Kinetics Force Simulation Enzymes Molecular Dynamics Molecules Experiments Kd Codon Peptides Tunnels Amino Acids Viruses Proteome Molecule Folding Antibodies Angiotensins Angiotensin Converting Enzyme 2 Thermodynamics

Most Recent Papers

RiboA: a web application to identify ribosome A-site locations in ribosome profiling data

Danying Shao, Nabeel Ahmed, Nishant Soni, Edward P. O’Brien, 2021, BMC Bioinformatics

The driving force for co-translational protein folding is weaker in the ribosome vestibule due to greater water ordering

Quyen V. Vu, Yang Jiang, Mai Suan Li, Edward P. O'Brien, 2021, Chemical Science on p. 11851-11857

Electrostatic Interactions Explain the Higher Binding Affinity of the CR3022 Antibody for SARS-CoV-2 than the 4A8 Antibody

Hung Nguyen, Pham Dang Lan, Daniel A. Nissley, Edward P. O'Brien, Mai Suan Li, 2021, Journal of Physical Chemistry B on p. 7368-7379

Mechanical Forces Have a Range of Effects on the Rate of Ribosome Catalyzed Peptidyl Transfer Depending on Direction

Yang Jiang, Edward P. O’Brien, 2021, Journal of Physical Chemistry B on p. 7128-7136

Genomic and experimental evidence that ALKATI does not predict single agent sensitivity to ALK inhibitors

H Inam, I Sokirniy, Y Rao, A Shah, F Naeemikia, Edward O'Brien, C Dong, D McCandlish, J Pritchard, 2021, on p. 103343

Combinations of slow translating codon clusters can increase mRNA half-life in S. cerevisiae

A Sharma, A Schiber, B Bukau, G Kramer, Edward O'Brien, 2021,

Ribosome elongation kinetics are coupled to electrostatic force generation

S Leininger, J Rodriguez, Q Vu, Y Jiang, M Li, C Deutsch, Edward O'Brien, 2021, on p. 3223 – 3235

Pairs of amino acids at the P- and A-sites of the ribosome predictably and causally modulate translation-elongation rates: Amino acid pairs module translation-elongation rates

Nabeel Ahmed, Ulrike A. Friedrich, Pietro Sormanni, Prajwal Ciryam, Naomi S. Altman, Bernd Bukau, Günter Kramer, Edward P. O'Brien, 2020, Journal of Molecular Biology

Does SARS-CoV-2 bind to human ACE2 more strongly than does SARS-CoV?

Hoang Linh Nguyen, Pham Dang Lan, Nguyen Quoc Thai, Daniel A. Nissley, Edward P. O'Brien, Mai Suan Li, 2020, Journal of Physical Chemistry B on p. 7336-7347

Forcing the ribosome to change its message

Sarah E. Leininger, Carol Deutsch, Edward P. O’Brien, 2020, Journal of Biological Chemistry on p. 6809-6810

Most-Cited Papers

Cotranslational Protein Folding inside the Ribosome Exit Tunnel

Ola B. Nilsson, Rickard Hedman, Jacopo Marino, Stephan Wickles, Lukas Bischoff, Magnus Johansson, Annika Müller-Lucks, Fabio Trovato, Joseph D. Puglisi, Edward P. O'Brien, Roland Beckmann, Gunnar von Heijne, 2015, Cell Reports on p. 1533-1540

Profiling Ssb-Nascent Chain Interactions Reveals Principles of Hsp70-Assisted Folding

Kristina Döring, Nabeel Ahmed, Trine Riemer, Harsha Garadi Suresh, Yevhen Vainshtein, Markus Habich, Jan Riemer, Matthias P. Mayer, Edward P. O'Brien, Günter Kramer, Bernd Bukau, 2017, Cell on p. 298-311.e20

Prediction of variable translation rate effects on cotranslational protein folding

Edward P. O'Brien, Michele Vendruscolo, Christopher M. Dobson, 2012, Nature Communications

Understanding the influence of codon translation rates on cotranslational protein folding

Edward P. O'Brien, Prajwal Ciryam, Michele Vendruscolo, Christopher M. Dobson, 2014, Accounts of Chemical Research on p. 1536-1544

Trigger factor slows Co-translational folding through kinetic trapping while sterically protecting the nascent chain from aberrant cytosolic interactions

Edward P. O'Brien, John Christodoulou, Michele Vendruscolo, Christopher M. Dobson, 2012, Journal of the American Chemical Society on p. 10920-10932

Kinetic modelling indicates that fast-translating codons can coordinate cotranslational protein folding by avoiding misfolded intermediates

Edward P. O'brien, Michele Vendruscolo, Christopher M. Dobson, 2014, Nature Communications

Protein folding

D. Thirumalai, Zhenxing Liu, Edward P. O'Brien, Govardhan Reddy, 2013, Current Opinion in Structural Biology on p. 22-29

Effects of pH on proteins

Edward P. O'Brien, Bernard R. Brooks, D. Thirumalai, 2012, Journal of the American Chemical Society on p. 979-987

Does SARS-CoV-2 bind to human ACE2 more strongly than does SARS-CoV?

Hoang Linh Nguyen, Pham Dang Lan, Nguyen Quoc Thai, Daniel A. Nissley, Edward P. O'Brien, Mai Suan Li, 2020, Journal of Physical Chemistry B on p. 7336-7347

Timing is everything

Daniel A. Nissley, Edward P. Obrien, 2014, Journal of the American Chemical Society on p. 17892-17898