Ashley Shay

Director, Metabolomics Core Facility

Ashley Shay

Research Summary

The implementation and expansion of liquid chromatography tandem mass spectrometry for the structural elucidation of novel lipid mediators and metabolites in various inflammatory diseases coupled with diverse cellular and in vivo approaches to determine functionality.

Huck Affiliations

Most Recent Publications

Library Enabling Annotation of Botanical Natural Products (LEAFBot): An Open-Access Library of Mass Spectrometry Fragmentation Spectra for Plant Metabolites

Victoria Anderson, Madhusha Ranaweera, Alan Jarmusch, Ashley Shay, Daniel Todd, Nadja Cech, J Kellogg, 2025, Journal of the American Society for Mass Spectrometry

Upregulated selenoprotein I during lipopolysaccharide-induced B cell activation promotes lipidomic changes and is required for effective differentiation into IgM-secreting plasma B cells

Chi Ma, FuKun Hoffmann, Ashley E. Shay, Imhoi Koo, Kathy Green, William Green, Peter Hoffmann, 2024, Journal of Leukocyte Biology on p. 6-17

Selenoprotein I is indispensable for ether lipid homeostasis and proper myelination

Lance G.A. Nunes, Chi Ma, Fu Kun W. Hoffmann, Ashley E. Shay, Matthew W. Pitts, Peter R. Hoffmann, 2024, Journal of Biological Chemistry

Eosinophil Phenotypes Are Functionally Regulated by Resolvin D2 during Allergic Lung Inflammation

Thayse R. Brüggemann, Hong Yong Peh, Luciana P. Tavares, Julie Nijmeh, Ashley E. Shay, Rafael M. Rezende, Toby B. Lanser, Charles N. Serhan, Bruce D. Levy, 2023, American Journal of Respiratory Cell and Molecular Biology on p. 666-677

Resolvin D1 prevents injurious neutrophil swarming in transplanted lungs

Wenjun Li, Hailey M. Shepherd, Yuriko Terada, Ashley E. Shay, Amit I. Bery, Andrew E. Gelman, Kory J. Lavine, Charles N. Serhan, Daniel Kreisel, 2023, Proceedings of the National Academy of Sciences of the United States of America on p. e2302938120

Transcellular biosynthesis of leukotriene B<sub>4</sub> orchestrates neutrophil swarming to fungi

Alex Hopke, Tian Lin, Allison K. Scherer, Ashley E. Shay, Kyle D. Timmer, Brittany Wilson-Mifsud, Michael K. Mansour, Charles N. Serhan, Daniel Irimia, Bryan P. Hurley, 2022, iScience

Periodontal Stem Cells Synthesize Maresin Conjugate in Tissue Regeneration 3

A. Rakian, R. Rakian, A. E. Shay, C. N. Serhan, T. E. Van Dyke, 2022, Journal of Dental Research on p. 1205-1213

A newly synthesized 17-epi-NeuroProtectin D1/17-epi-Protectin D1: Authentication and functional regulation of Inflammation-Resolution

Kajal Hamidzadeh, Jodi Westcott, Nicholas Wourms, Ashley E. Shay, Anand Panigrahy, Michael J. Martin, Robert Nshimiyimana, Charles N. Serhan, 2022, Biochemical Pharmacology

Cysteinyl Maresins Reprogram Macrophages to Protect Mice from Streptococcus pneumoniae after Influenza A Virus Infection

Luciana P. Tavares, Thayse R. Brüggemann, Rafael M. Rezende, Marina G. Machado, R. Elaine Cagnina, Ashley E. Shay, Cristiana C. Garcia, Julie Nijmeh, Mauro M. Teixeira, Bruce D. Levy, 2022, mBio

Human leukocytes selectively convert 4S,5S-epoxy-resolvin to resolvin D3, resolvin D4, and a cys-resolvin isomer

Ashley E. Shay, Robert Nshimiyimana, Bengt Samuelsson, Nicos A. Petasis, Jesper Z. Haeggstrom, Charles N. Serhan, 2021, Proceedings of the National Academy of Sciences of the United States of America

Most-Cited Papers

Selenium and inflammatory bowel disease

A Kudva, Ashley Shay, K. Sandeep Prabhu, 2015, American Journal of Physiology on p. G71-G77

A New E-Series Resolvin: RvE4 Stereochemistry and Function in Efferocytosis of Inflammation-Resolution

Stephania Libreros, Ashley E. Shay, Robert Nshimiyimana, David Fichtner, Michael J. Martin, Nicholas Wourms, Charles N. Serhan, 2021, Frontiers in Immunology

Protectins PCTR1 and PD1 Reduce Viral Load and Lung Inflammation During Respiratory Syncytial Virus Infection in Mice

Katherine H. Walker, Nandini Krishnamoorthy, Thayse R. Brüggemann, Ashley E. Shay, Charles N. Serhan, Bruce D. Levy, 2021, Frontiers in Immunology

Selenoprotein expression in macrophages is critical for optimal clearance of parasitic helminth Nippostrongylus brasiliensis

S Nelson, Ashley Shay, J James, B Carlson, J Urban, Jr, Joseph F. Urban, K. Sandeep Prabhu, 2016, Journal of Biological Chemistry on p. 2787-2798

Human leukocytes selectively convert 4S,5S-epoxy-resolvin to resolvin D3, resolvin D4, and a cys-resolvin isomer

Ashley E. Shay, Robert Nshimiyimana, Bengt Samuelsson, Nicos A. Petasis, Jesper Z. Haeggstrom, Charles N. Serhan, 2021, Proceedings of the National Academy of Sciences of the United States of America

Biosynthetic metabolomes of cysteinyl-containing immunoresolvents

Charlotte C. Jouvene, Ashley E. Shay, Mieke A. Soens, Paul C. Norris, Jesper Z. Haeggström, Charles N. Serhan, 2019, FASEB Journal on p. 13794-13807

IL-4 up-regulates cyclooxygenase-1 expression in macrophages

Ashley Shay, B Diwakar, B Guan, V Narayan, Joseph F. Urban, J Urban, Jr, K. Sandeep Prabhu, 2017, Journal of Biological Chemistry on p. 14544-14555

Selenium-dependent metabolic reprogramming during inflammation and resolution

A Korwar, A Hossain, T Lee, Ashley Shay, V Basrur, K Conlon, Philip B. Smith, B Carlson, Howard M. Salis, Andrew D. Patterson, K. Sandeep Prabhu, 2021, Journal of Biological Chemistry on p. 100410

Stereoselective Synthesis and Structural Confirmation of the Specialized Pro-Resolving Mediator Resolvin E4

Amalie Føreid Reinertsen, Karoline Gangestad Primdahl, Ashley Elizabeth Shay, Charles Nicholas Serhan, Trond Vidar Hansen, Marius Aursnes, 2021, Journal of Organic Chemistry on p. 3535-3545

Transcellular biosynthesis of leukotriene B<sub>4</sub> orchestrates neutrophil swarming to fungi

Alex Hopke, Tian Lin, Allison K. Scherer, Ashley E. Shay, Kyle D. Timmer, Brittany Wilson-Mifsud, Michael K. Mansour, Charles N. Serhan, Daniel Irimia, Bryan P. Hurley, 2022, iScience