Amie Boal

Professor of Chemistry and of Biochemistry and Molecular Biology

Amie Boal

Research Summary

The structural differences between members of large metalloenzyme superfamilies that share common features but promote different reactions or use distinct cofactors.

Huck Affiliations

Links

Publication Tags

Enzymes Oxygenases Iron Proteins Hydrogen Carbon Arginine X Rays Glutarates Protein Hydroxylation Ketoglutaric Acids Mixed Function Oxygenases Ligands Catalytic Domain Carbon Atom S Adenosylmethionine Hydrogen Atom Chemical Transformation Oxygen Ferryl Iron Halogenation Cofactor Alkenes Substrates

Most Recent Publications

Discovery, structure and mechanism of a tetraether lipid synthase

Cody T. Lloyd, David F. Iwig, Bo Wang, Matteo Cossu, William W. Metcalf, Amie K. Boal, Squire J. Booker, 2022, Nature on p. 197-203

Use of Noncanonical Tyrosine Analogues to Probe Control of Radical Intermediates during Endoperoxide Installation by Verruculogen Synthase (FtmOx1)

Chi Yun Lin, Angel L. Muñoz Hernández, Tatiana N. Laremore, Alexey Silakov, Carsten Krebs, Amie K. Boal, J. Martin Bollinger, 2022, ACS Catalysis on p. 6968-6979

Structural insights into auxiliary cofactor usage by radical S-adenosylmethionine enzymes

Vivian Robert Jeyachandran, Amie K. Boal, 2022, Current Opinion in Chemical Biology

Progress Toward Understanding Protein Control of Reaction Outcome in the Diverse Reactivity of Iron(II)- and 2-Oxoglutarate-dependent Oxygenases

J. M. Bollinger, Carsten Krebs, Amie K. Boal, Alexey Silakov, John C. Price, Megan L. Matthews, Wei Chen Chang, Ryan J. Martinie, Juan Pan, Noah P. Dunham, Eliott S. Wenger, Rachelle A. Copeland, Chi Yun Lin, 2022, FASEB Journal

Substrate-Triggered μ-Peroxodiiron(III) Intermediate in the 4-Chloro- l -Lysine-Fragmenting Heme-Oxygenase-like Diiron Oxidase (HDO) BesC: Substrate Dissociation from, and C4 Targeting by, the Intermediate

Molly J. McBride, Mrutyunjay A. Nair, Debangsu Sil, Jeffrey W. Slater, Monica E. Neugebauer, Michelle C.Y. Chang, Amie K. Boal, Carsten Krebs, J. Martin Bollinger, 2022, Biochemistry on p. 689-702

Structure of a B<sub>12</sub>-dependent radical SAM enzyme in carbapenem biosynthesis

Hayley L. Knox, Erica K. Sinner, Craig A. Townsend, Amie K. Boal, Squire J. Booker, 2022, Nature on p. 343-348

Structural Basis for an Unprecedented Enzymatic Alkylation in Cylindrocyclophane Biosynthesis

Nathaniel R. Braffman, Terry B. Ruskoski, Katherine M. Davis, Nathaniel R. Glasser, Cassidy Johnson, C. Denise Okafor, Amie K. Boal, Emily P. Balskus, 2022, eLife

The periodic table of ribonucleotide reductases

Terry B. Ruskoski, Amie K. Boal, 2021, Journal of Biological Chemistry

An Iron(IV)-Oxo Intermediate Initiating l -Arginine Oxidation but Not Ethylene Production by the 2-Oxoglutarate-Dependent Oxygenase, Ethylene-Forming Enzyme

Rachelle Copeland, Katherine Davis, Tokufu Shoda, Elizabeth Blaesi, A Boal, Carsten Krebs, J. Bollinger, 2021, Journal of the American Chemical Society on p. 2293-2303

Structure and assembly of the diiron cofactor in the heme-oxygenase–like domain of the N-nitrosourea–producing enzyme SznF

M McBride, S Pope, K Hu, C. Denise Okafor, C Denise Okafor, E Balskus, J Bollinger, J. Martin Bollinger, Amie K. Boal, 2021, Proceedings of the National Academy of Sciences of the United States of America

Most-Cited Papers

Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers

Franklin D. Fuller, Sheraz Gul, Ruchira Chatterjee, E. Sethe Burgie, Iris D. Young, Hugo Lebrette, Vivek Srinivas, Aaron S. Brewster, Tara Michels-Clark, Jonathan A. Clinger, Babak Andi, Mohamed Ibrahim, Ernest Pastor, Casper De Lichtenberg, Rana Hussein, Christopher J. Pollock, Miao Zhang, Claudiu A. Stan, Thomas Kroll, Thomas Fransson, Clemens Weninger, Markus Kubin, Pierre Aller, Louise Lassalle, Philipp Bräuer, Mitchell D. Miller, Muhamed Amin, Sergey Koroidov, Christian G. Roessler, Marc Allaire, Raymond G. Sierra, Peter T. Docker, James M. Glownia, Silke Nelson, Jason E. Koglin, Diling Zhu, Matthieu Chollet, Sanghoon Song, Henrik Lemke, Mengning Liang, Dimosthenis Sokaras, Roberto Alonso-Mori, Athina Zouni, Johannes Messinger, Uwe Bergmann, Amie K. Boal, J. Martin Bollinger, Carsten Krebs, Martin Högbom, George N. Phillips, Richard D. Vierstra, Nicholas K. Sauter, Allen M. Orville, Jan Kern, Vittal K. Yachandra, Junko Yano, 2017, Nature Methods on p. 443-449

Mechanism of the C5 stereoinversion reaction in the biosynthesis of carbapenem antibiotics

Wei Chen Chang, Yisong Guo, Chen Wang, Susan E. Butch, Amy C. Rosenzweig, Amie K. Boal, Carsten Krebs, J. Martin Bollinger, 2014, Science on p. 1140-1144

Structural basis for halogenation by iron-and 2-oxo-glutarate-dependent enzyme WelO5

Andrew J. Mitchell, Qin Zhu, Ailiena O. Maggiolo, Nikhil R. Ananth, Matthew L. Hillwig, Xinyu Liu, Amie K. Boal, 2016, Nature Chemical Biology on p. 636-640

Visualizing the reaction cycle in an Iron(II)- and 2-(Oxo)-glutarate-dependent hydroxylase

Andrew J. Mitchell, Noah P. Dunham, Ryan J. Martinie, Jonathan A. Bergman, Christopher J. Pollock, Kai Hu, Benjamin D. Allen, Wei Chen Chang, Alexey Silakov, J. Martin Bollinger, Carsten Krebs, Amie K. Boal, 2017, Journal of the American Chemical Society on p. 13830-13836

Two Distinct Mechanisms for C-C Desaturation by Iron(II)- and 2-(Oxo)glutarate-Dependent Oxygenases: Importance of α-Heteroatom Assistance

Noah P. Dunham, Wei Chen Chang, Andrew J. Mitchell, Ryan J. Martinie, Bo Zhang, Jonathan A. Bergman, Lauren J. Rajakovich, Bo Wang, Alexey Silakov, Carsten Krebs, Amie K. Boal, J. Martin Bollinger, 2018, Journal of the American Chemical Society on p. 7116-7126

An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin

Tai L. Ng, Roman Rohac, Andrew J. Mitchell, Amie K. Boal, Emily P. Balskus, 2019, Nature on p. 94-99

AgHalo: A Facile Fluorogenic Sensor to Detect Drug-Induced Proteome Stress

Yu Liu, Matthew Fares, Noah P. Dunham, Zi Gao, Kun Miao, Xueyuan Jiang, Samuel S. Bollinger, Amie K. Boal, Xin Zhang, 2017, Angewandte Chemie - International Edition on p. 8672-8676

Mechanisms of 2-oxoglutarate-dependent oxygenases: The hydroxylation paradigm and beyond

J. Martin Bollinger, Wei Chen Chang, Megan L. Matthews, Ryan J. Martinie, Amie K. Boal, Carsten Krebs, 2015, on p. 95-122

The Cation−π Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification

Yu Liu, Kun Miao, Noah P. Dunham, Hongbin Liu, Matthew Fares, Amie K. Boal, Xiaosong Li, Xin Zhang, 2017, Biochemistry on p. 1585-1595

Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA

Erica L. Schwalm, Tyler L. Grove, Squire J. Booker, Amie K. Boal, 2016, Science on p. 309-312