Amie Boal

Associate Professor of Chemistry and of Biochemistry and Molecular Biology

Amie Boal

Research Summary

The structural differences between members of large metalloenzyme superfamilies that share common features but promote different reactions or use distinct cofactors.

Huck Affiliations


Publication Tags

Iron Oxygenases Enzymes Proteins Hydroxylation Glutarates Substrates Carbon Arginine Hydrogen X Rays Decarboxylation Mixed Function Oxygenases Ligands Fatty Acids Oxidoreductases Protein Carbon Atom Catalytic Domain Ferryl Iron Ketoglutaric Acids Streptozocin Cofactor Heme Oxygenase (Decyclizing) Dioxygenases

Most Recent Papers

The periodic table of ribonucleotide reductases

Terry B. Ruskoski, Amie K. Boal, 2021, Journal of Biological Chemistry

An Iron(IV)-Oxo Intermediate Initiating l -Arginine Oxidation but Not Ethylene Production by the 2-Oxoglutarate-Dependent Oxygenase, Ethylene-Forming Enzyme

Rachelle Copeland, Katherine Davis, Tokufu Shoda, Elizabeth Blaesi, A Boal, Carsten Krebs, J. Bollinger, 2021, Journal of the American Chemical Society on p. 2293-2303

Intrinsically disordered substrates dictate SPOP subnuclear localization and ubiquitination activity

Emery T. Usher, Nafiseh Sabri, Roman Rohac, Amie K. Boal, Tanja Mittag, Scott A. Showalter, 2021, Journal of Biological Chemistry

Biochemical Approaches to Probe the Role of the Auxiliary Iron-Sulfur Cluster of Lipoyl Synthase from Mycobacterium Tuberculosis

Vivian Robert Jeyachandran, Jay V. Pendyala, Erin L. McCarthy, Amie K. Boal, Squire J. Booker, 2021, on p. 307-332

Structure-function relationships in assembly of the radical-initiating cofactors of class la-e ribonucleotide reductases

Hannah R. Rose, Gavin M. Palowitch, Kai Hu, Avani Gandhi, Amie K. Boal, 2020, on p. 415-441

Emerging structural and functional diversity in proteins with dioxygen-reactive dinuclear transition metal cofactors

Lauren J. Rajakovich, Bo Zhang, Molly J. McBride, Amie K. Boal, Carsten Krebs, J. Martin Bollinger, 2020, Comprehensive Natural Products III: Chemistry and Biology on p. 215-250

A peroxodiiron(III/III) intermediate mediating both n-hydroxylation steps in biosynthesis of the n-nitrosourea pharmacophore of streptozotocin by the multi-domain metalloenzyme SznF

Molly J. McBride, Debangsu Sil, Tai L. Ng, Anne Marie Crooke, Grace E. Kenney, Christina R. Tysoe, Bo Zhang, Emily P. Balskus, Amie K. Boal, Carsten Krebs, J. Martin Bollinger, 2020, Journal of the American Chemical Society on p. 11818-11828

The immune system mimics a pathogen

Amie K. Boal, 2019, Science on p. 574-575

Structure of a Ferryl Mimic in the Archetypal Iron(II)- And 2-(Oxo)-glutarate-Dependent Dioxygenase, TauD

Katherine M. Davis, Madison Altmyer, Ryan J. Martinie, Irene Schaperdoth, Carsten Krebs, J. Martin Bollinger, Amie K. Boal, 2019, Biochemistry on p. 4218-4223

Substrate-triggered formation of a peroxo-Fe<sub>2</sub>(III/III) intermediate during fatty acid decarboxylation by UndA

Bo Zhang, Lauren J. Rajakovich, Devon Van Cura, Elizabeth J. Blaesi, Andrew J. Mitchell, Christina R. Tysoe, Xuejun Zhu, Bennett R. Streit, Zhe Rui, Wenjun Zhang, Amie K. Boal, Carsten Krebs, J. Martin Bollinger, 2019, Journal of the American Chemical Society on p. 14510-14514

Most-Cited Papers

Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers

Franklin D. Fuller, Sheraz Gul, Ruchira Chatterjee, E. Sethe Burgie, Iris D. Young, Hugo Lebrette, Vivek Srinivas, Aaron S. Brewster, Tara Michels-Clark, Jonathan A. Clinger, Babak Andi, Mohamed Ibrahim, Ernest Pastor, Casper De Lichtenberg, Rana Hussein, Christopher J. Pollock, Miao Zhang, Claudiu A. Stan, Thomas Kroll, Thomas Fransson, Clemens Weninger, Markus Kubin, Pierre Aller, Louise Lassalle, Philipp Bräuer, Mitchell D. Miller, Muhamed Amin, Sergey Koroidov, Christian G. Roessler, Marc Allaire, Raymond G. Sierra, Peter T. Docker, James M. Glownia, Silke Nelson, Jason E. Koglin, Diling Zhu, Matthieu Chollet, Sanghoon Song, Henrik Lemke, Mengning Liang, Dimosthenis Sokaras, Roberto Alonso-Mori, Athina Zouni, Johannes Messinger, Uwe Bergmann, Amie K. Boal, J. Martin Bollinger, Carsten Krebs, Martin Högbom, George N. Phillips, Richard D. Vierstra, Nicholas K. Sauter, Allen M. Orville, Jan Kern, Vittal K. Yachandra, Junko Yano, 2017, Nature Methods on p. 443-449

Mechanism of the C5 stereoinversion reaction in the biosynthesis of carbapenem antibiotics

Wei Chen Chang, Yisong Guo, Chen Wang, Susan E. Butch, Amy C. Rosenzweig, Amie K. Boal, Carsten Krebs, J. Martin Bollinger, 2014, Science on p. 1140-1144

Structural basis for halogenation by iron- and 2-oxo-glutarate-dependent enzyme WelO5

Andrew J. Mitchell, Qin Zhu, Ailiena O. Maggiolo, Nikhil R. Ananth, Matthew L. Hillwig, Xinyu Liu, Amie K. Boal, 2016, Nature Chemical Biology on p. 636-640

Visualizing the reaction cycle in an Iron(II)- and 2-(Oxo)-glutarate-dependent hydroxylase

Andrew J. Mitchell, Noah P. Dunham, Ryan J. Martinie, Jonathan A. Bergman, Christopher J. Pollock, Kai Hu, Benjamin D. Allen, Wei Chen Chang, Alexey Silakov, J. Martin Bollinger, Carsten Krebs, Amie K. Boal, 2017, Journal of the American Chemical Society on p. 13830-13836

Two Distinct Mechanisms for C-C Desaturation by Iron(II)- and 2-(Oxo)glutarate-Dependent Oxygenases

Noah P. Dunham, Wei Chen Chang, Andrew J. Mitchell, Ryan J. Martinie, Bo Zhang, Jonathan A. Bergman, Lauren J. Rajakovich, Bo Wang, Alexey Silakov, Carsten Krebs, Amie K. Boal, J. Martin Bollinger, 2018, Journal of the American Chemical Society on p. 7116-7126

Mechanisms of 2-oxoglutarate-dependent oxygenases: The hydroxylation paradigm and beyond

J. Martin Bollinger, Wei Chen Chang, Megan L. Matthews, Ryan J. Martinie, Amie K. Boal, Carsten Krebs, 2015, on p. 95-122

AgHalo: A facile fluorogenic sensor to detect drug induced proteome stress

Yu Liu, Matthew Fares, Noah P. Dunham, Zi Gao, Kun Miao, Xueyuan Jiang, Samuel S. Bollinger, Amie K. Boal, Xin Zhang, 2017, Angewandte Chemie - International Edition on p. 8672-8676

An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin

Tai L. Ng, Roman Rohac, Andrew J. Mitchell, Amie K. Boal, Emily P. Balskus, 2019, Nature on p. 94-99

Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA

Erica L. Schwalm, Tyler L. Grove, Squire J. Booker, Amie K. Boal, 2016, Science on p. 309-312

The cation-Pi interaction enables a halo-tag fluorogenic probe for fast no-wash live cell imaging and gel-free protein quantification

Yu Liu, Kun Miao, Noah P. Dunham, Hongbin Liu, Matthew Fares, Amie K. Boal, Xiaosong Li, Xin Zhang, 2017, Biochemistry on p. 1585-1595