Nikki Shariat (Edward Dudley lab, Penn State): Introduction to CRISPR. Yinong Yang (Penn State): Implementing CRISPR/Cas9 genome editing in plants. Kate O Connor Giles (Wisconsin): Implementing CRISPR/Cas9 genome editing in Drosophila.

Format: We will start with a short talk to bring everyone up to speed on the bacterial CRISPR/Cas9 system. We will then have two talks on using this guided nuclease to introduce changes into the genome of model systems._ Background: Genome engineering with CRISPR is being rapidly implemented in all model systems. It seems like a very easy way to generate targeted deletions, and in more sophisticated versions, to insert sequences at specific genomic sites. In the workshop we will hear how it has been implemented in model systems, with the idea of determining if and how it might be useful for our own research projects.

Contact

  Melissa Rolls
  mur22@psu.edu
  814-867-1395