Introduces users to commonly used proteomics techniques and providing basic skills that can be applied in your research projects.
Proteomics short course
August 3, 2009 @ 10:00 am to August 7, 2009 @ 05:00 pm
3 Althouse Laboratory
Proteomics and Mass Spectrometry Core Facility is sponsoring a week-long Proteomics workshop aimed at introducing users to commonly used proteomics techniques and basic skills which can be incorporate into your research projects._ Techniques covered 2D Gel Electrophoresis image analysis automated spot cutting in-gel digestion MALDI-TOF analysis 1D and 2D nanoLC-MS/MS analysis protein database searching Enrollment Enrollment is limited to 6 people. If space is not available in this workshop, you may ask to be included in the waiting list for upcoming workshops which are planned to be held quarterly. Fees The fee for the workshop is $500 per person payable by IDCC (credited to 430-46 1001J, send to user ID: qub8) Questions / requests Please e-mail all requests to ms@huck.psu.edu Schedule Following schedule is_tentative. Workshops run from 9:00 AM to 4:00 PM daily. Day 1 - Mon Aug 3, 2009 Background Lecture Biological Mass Spectrometry Sample Preparation for 2D-GE Day 2 - Tues Aug 4, 2009 First dimension of 2D-GE, Isoelectric Focusing (IEF) Protein MW determination by LC-MS Day 3 - Wed Aug 5, 2009 Second dimension of 2D-GE, SDS-PAGE Gel staining Scanning with densitometer and laser scanner Image Analysis by PDQuest Day 4 - Thurs Aug 6, 2009 Automated spot cutting In-gel digestion Day 5 - Fri Aug 7, 2009 Peptide Mass Fingerprinting by MALDI-TOF Protein analysis by nanoLC-MS/MS and identification by protein database search _