Amnis FlowSight – Imaging Flow Cytometer

The Amnis FlowSight is an imaging flow cytometer that can produce fluorescent, darkfield and brightfield images of every cell that runs through the instrument.

Flow Cytometry Facility

W124A Millennium Science Complex
491 Pollock Rd
University Park, PA 16802

Phone: 814-865-6595

AMNIS FlowSight Imaging Cytometer

Uses

  • Cell Cycle and Mitosis Analysis
  • Functional Assays (Phagocytosis etc.)
  • Internalization; Co-localization
  • Shape Changes; Spot Counting
  • Cell-Cell Interactions – Immune Synapse
  • Oceanography and Water – Phytoplankton analysis

Specifications

  • 20 X magnification
  • Four excitation lasers (Violet 405nm, Blue 488nm, Red 642nm and 785nm-SSC)
  • Up to 10 fluorescent parameters in addition to brightfield
  • 785nm laser-based SSC
  • Quantitative Imaging – IDEAS analysis software
  • Numeric Aperture: 0.6
  • Pixel Size: 1.0 x 1.0 µm
  • Field of View: 60 x 256 µm
  • Imaging Rate: 4,000 Obj/Sec

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Flow Cytometry Core Facility (RRID: SCR_024460) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.