Genomics Core Facility

Agilent Bioanalyzer

The Agilent Bioanalyzer is a capillary electrophoresis device used by our lab for QC of RNA and DNA samples. We support the use of RNA Nano, RNA Pico, DNA 1000, and DNA HS chips.

Specification

  • 12 samples can be assayed on a single RNA Nano or DNA 1000 chip.
  • 11 samples can be assayed on a single RNA Pico or DNA HS chip.
  • NGS libraries or fragmented DNA samples from 50 nt – 7000 nt at concentrations of 100 pg/ul – 10 ng/ul can be analyzed on the DNA HS chip.
  • DNA from 50 nt – 1000 nt and concentrations of 0.5 ng/ul – 50/ng/ul can be analyzed on the DNA 1000 chip
  • RNA samples at concentrations of 25 ng/ul – 500 ng/ul can be analyzed on the RNA Nano chip.
  • RNA samples at concentrations of 50 pg/ul – 5000 pg/ul can be analyzed on the RNA Pico chip.
  • An objective measure of RNA quality, the RNA Integrity Number (RIN) can be determined for total RNA samples run on either the RNA Nano or RNA Pico chip.

Details

  • Please provide us a minimum of 5 ul per sample. This allows us to check the concentration of your sample prior to assaying it on the Bioanalyzer. This also provides enough sample so that it can be re-run if necessary. We are happy to return the unused portion of your samples.
  • Provide your samples in standard 0.5 or 1.5 ml microfuge tubes. No 0.2 ml PCR tubes or PCR strip tubes allowed.

Used for a variety of applications:

  • NGS library QC
  • Total RNA RIN determination
  • Assessment of rRNA depletion
  • Accurate size determination and quantification of DNA fragments.

This instrument is operated by Genomics Core Facility staff in conjunction with next-generation sequencing services