The Huck Institutes of the Life Sciences

Microscopy and Cytometry Facility

Providing services in all aspects of optical and electron microscopy, histology, flow cytometry, and cell sorting

The Facility is specialized in four clusters – optical microscopy, electron microscopy, histology and flow cytometry – equipped with confocal microscopes, research fluorescence microscopes, transmission- and scanning electron microscopes, flow cytometers and a cell sorter, and whose research staff is engaged in experimentation, training, project collaboration and consultation.

 

Picture of the Month

Detection of xyloglucan at the surface of onion epidermal cell walls by use of a carbohydrate binding module (CBM76) tagged with 5-nm nanogold (white and colored spheres). The ‘chain of pearls’ appear to delimit xyloglucan chains that in some cases assume an extended conformation that follows cellulose microfibrils and in other cases appear more like a random coil. Gold particles were hand-colored to suggest particles that may be bound to the same chain. The image was collected by a Zeiss Sigma field-emission scanning electron microscopy (FESEM) with back-scattered electron (BSE) detection to highlight the gold particles. Image width 663 nm, height 533 nm. This image is from Zheng, Y., Wang, X., Chen, Y., Wagner, E. and Cosgrove, D.J. (2017) Xyloglucan in the primary cell wall: Assessment by FESEM, selective enzyme digestions and nanogold affinity tags. Plant J, online before print. http://onlinelibrary.wiley.com/doi/10.1111/tpj.13778/full