Student

Rishi Ravichandran

Graduate Student

Adviser: Francesco Di Gioia

Email rbr5349@psu.edu
Pronouns He/Him

Overview

Molecular mechanisms underlying iron-zinc interaction and biofortification

Centers & Institutes

Plant Institute

Plant Biology IGDP

Researchers have worked out the mechanism of transcription pausing in some bacteria by determining the structures of RNA polymerase in a complex with a DNA template, an RNA product, and a protein factor using cryo-electron microscopy (cryo-EM). The nucleotide addition cycle of bacterial RNA polymerase (RNAP) is depicted by cartoon models of template DNA (tDNA, green), RNA (red), trigger loop (TL, magenta), bridge helix (BH, blue), incoming NTP (red) and Mg2+ (yellow spheres) together with the conformational changes of RNAP (swiveling, folding Trigger loop) associated with each step of the nucleotide addition cycle. NusG and non-template DNA (ntDNA, light green) interaction (center panel) inhibits the RNAP swiveling, the Trigger loop folding allosterically thus pauses RNA synthesis. Credit: Murakami Laboratory / Penn State. Creative Commons

February 09, 2023

DNA stuck in the gears of the RNA production machine

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