Uses
- Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules
- Binding affinity (Kd), Enthalpy (ΔH), Entropy (ΔS), Stoichiometry (n)
- Dimer dissociation
- Enzyme kinetics
- Assessment of molecular structure changes on binding mechanisms
Specifications
- Operating temperature: 2°C to 80°C
- Autosampler storage temperature: 4°C to ambient
- Response time: 3.3 seconds
- Cell design: 185uL active volume cylindrical cell
- Injection syringe: up to 270uL
- Usable syringe volume: 100µl
- Smallest injection size: 0.1µl
- Stirring speed: up to 200 rpm
- Full Automation 24K Gold cell
Details
The affinity ITC is fully automated, Users can set up samples in 96 well plate in a controlled temperature autosampler. Automated cleaning can also be programmed to reduce contamination between runs. Optional manual mode is available for using even less volume. Affinity ITC is located in a glove box in order to run oxygen sensitive samples.
Acknowledgement
Users of this instrument are required to acknowledge the Huck Institutes’ Biomolecular Interactions Core Facility (RRID: SCR_024458) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.
Research reported here is supported by the NIH grant S10OD025145 to Neela Yennawar for the TA Instruments Low Volume AutoAffinity ITC housed in the Biomolecular Interactions Core Facility at Penn State's Huck Institutes of the Life Sciences.