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Qubit

The ThermoFisher Qubit is a fluorometer, in conjunction with associated reagents, which can accurately measure the concentration of RNA and DNA samples. While spectrophotometers can accurately measure the concentrations of pure preparations of DNA or RNA, the accuracy can be greatly effected by the presence of a number of contaminants including other nucleic acids. The Qubit utilizes the binding of fluorescent dyes specific to RNA or dsDNA to allow an accurate determination of concentration even in the presence of contaminants.

Genomics Facility

407 Chandlee Lab
209 Pollock Rd
University Park, PA 16802

Phone: 814-867-4067

Penn State shield logo featuring a stylized mountain lion in white and light blue on a blue gradient with white s-curve background.

Uses

  • Essential for accurately determining dsDNA concentration for input into NGS library construction.
  • Determination of total RNA concentration for input into NGS library construction.

Specifications

  • When assaying 2ul of sample, 0.1 ng/ul – 50ng/ul of dsDNA can be measured using the DNA HS assay.
  • When assaying 2ul of sample 1 ng/ul – 500 ng/ul of dsDNA can be measured using the DNA BR assay.
  • When assaying 2 ul of sample 2.5 ng/ul – 50 ng/ul of RNA can be measured using the RNA HS assay.

Details

  • Please provide us a minimum of 4 ul per sample.
  • Provide your samples in standard 0.5 or 1.5 ml microfuge tubes. No 0.2 ml PCR tubes or PCR strip tubes allowed.

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Genomics Core Facility (RRID: SCR_023645) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.