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PacBio Revio

The PacBio Revio is our new long-read sequencer and replaces our PacBio Sequel IIe

Genomics Facility

407 Chandlee Lab
209 Pollock Rd
University Park, PA 16802

Phone: 814-867-4067

Penn State shield logo featuring a stylized mountain lion in white and light blue on a blue gradient with white s-curve background.

Uses

  • De novo whole genome sequencing
  • Amplicon sequencing
  • Target enrichment
  • Iso-seq (RNA sequencing, Methylation detection, Fiber-seq)

Specifications

  • The Revio can run up to 4 SMRT cells at a time
  • Each SMRTcell can produce up to ~120 Gb of data
  • High Fidelity (HiFi) reads with >99% accuracy from templates of 1 – 20 kb in length can be generated from circular consensus sequencing
  • Multiplexing available for Iso-seq, amplicon, and bacterial genome sequencing
  • Each sample requires only 500 ng input

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Genomics Core Facility (RRID: SCR_023645) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.