Millipore Direct Detect Spectrometer

Uses

• Protein Quantitation
• Peptide Quantitation
• Detection of biomolecules including nucleic acids, lipids, and carbohydrates
• Quantification of long aliphatic chains in lipids and detergents

Specifications

• Samples are introduced using the assay-free sample card
• Each card contains 4 polymer membrane sample positions
• Time: 2 minutes per card
• Sample volume: 2 microliters
• Range: Protein 0.25–5.0 mg/ml; Lipid 0.1–10 mg/ml

Details

The Direct Detect spectrometer provides accurate and precise results even in the presence of detergent (SDS) and reducing agent (DTT). By measuring amide bonds in protein chains, the system accurately quantitates an intrinsic component of every protein without relying on amino acid composition, dye binding properties or reduction-oxidation (redox) potential.