MicroCal VP-capillary DSC

The VP-capillary DSC is the most sensitive, easy-to-use differential scanning calorimeter to study samples in solution, providing fast, accurate transition midpoint (Tm) determination and ΔH.

Biomolecular Interactions Facility

8 Althouse Lab
363 Science Dr
University Park, PA 16802

Phone: 814-863-9387

MicroCal VP-capillary DSC equipment

Uses

Study macromolecular folding stability:

  • Effects of protein mutagenesis and engineering
  • Effects of buffer, pH, salt, additives
  • Effects of post-translational modification
  • Reversibility of unfolding, by changes in ΔH
  • Presence of multiple unfolding domains
  • Stability contributions of individual domains
  • Formation of protein complexes
  • Stability of oligonucelotides
  • Effects of lipid, nucleic acid, or other biopolymer on protein stability

Specifications

  • Operating temperature range: -10°C to 130°C
  • Minimum response time (high gain): 5 seconds
  • Cell design: Tantalum 130 µl capillary type
  • Short term noise: 0.6µcal/°C
  • Baseline reliability: 1.5 µcal/°C

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Biomolecular Interactions Core Facility (RRID: SCR_024458) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.

Acknowledgement of the Biomolecular Interactions Core Facility must include the use of the core's unique RRID (SCR_024458)