Uses
The sequencing chemistry is similar across all Illumina platforms. Libraries compatible with one Illumina sequencer should be compatible with all Illumina sequencers.
Used for a variety of applications, including but not limited to:
- De novo whole genome sequencing
- Whole genome resequencing
- Exome sequencing
- RNA-Seq
- scRNA-Seq
- miRNA/small RNA-Seq
- ChIP-Seq
- RIP-Seq
- Degradome sequencing
Specifications
- Four output sizes available: P1, P2, P3, and P4 producing 100 million, 400 million, 1.2 billion reads, 1.8 billion reads (or read pairs) per run respectively
- P1 is available as 100, 300, or 600 cycle kits
- P2 is available as 100, 200, 300, or 600 cycle kits
- P3 is available as 100, 200, 300 cycle kits
- P2 is available as 50, 100, 200, 300 cycle kits
- One library pool per flow cell, no individual lane loading
- Sample multiplexing available up to 96-576 samples, depending on the application
Details
Data produced in the FASTQ format.
Acknowledgement
Users of this instrument are required to acknowledge the Huck Institutes’ Genomics Core Facility (RRID: SCR_023645) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.