Illumina NextSeq 2000

The Illumina NextSeq 2000 is our newest short-read sequencer, and has replaced the NextSeq 550.

Genomics Facility

407 Chandlee Lab
209 Pollock Rd
University Park, PA 16802

Phone: 814-867-4067

Illumina NextSeq 2000

Uses

The sequencing chemistry is similar across all Illumina platforms. Libraries compatible with one Illumina sequencer should be compatible with all Illumina sequencers.

Used for a variety of applications, including but not limited to:

  • De novo whole genome sequencing
  • Whole genome resequencing
  • Exome sequencing
  • RNA-Seq
  • scRNA-Seq
  • miRNA/small RNA-Seq
  • ChIP-Seq
  • RIP-Seq
  • Degradome sequencing

Specifications

  • Four output sizes available: P1, P2, P3, and P4 producing 100 million, 400 million, 1.2 billion reads, 1.8 billion reads (or read pairs) per run respectively
  • P1 is available as 100, 300, or 600 cycle kits
  • P2 is available as 100, 200, 300, or 600 cycle kits
  • P3 is available as 100, 200, 300 cycle kits
  • P2 is available as 50, 100, 200, 300 cycle kits
  • One library pool per flow cell, no individual lane loading
  • Sample multiplexing available up to 96-576 samples, depending on the application

Details

Data produced in the FASTQ format.

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Genomics Core Facility (RRID: SCR_023645) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.