Xin Zhang

Assistant Professor of Chemistry; Assistant Professor of Biochemistry and Molecular Biology; Paul Berg Early Career Professorship in the Eberly College of Science

Xin Zhang

Research Summary

Developing chemical tools to monitor cellular stresses that influence protein folding in real time. Deciphering how the energy landscapes associated with proper protein folding and function are regulated by the cellular folding environment. Examining how this regulation leads to significant biological consequences.

Huck Affiliations


Publication Tags

Proteins Fluorescence Sensors Proteome Agglomeration Molecules Signal Recognition Particle Chromophores Nanoparticles Proteostasis Dna Fluorophores Enzyme Activity Benzene Imaging Techniques Homeostasis Oxidation Reduction Gels Photolithography Escherichia Coli Cations Red Fluorescent Protein Cerium Oxide Pyridine Electrons

Most Recent Papers

A general strategy to control viscosity sensitivity of molecular rotor-based fluorophores

S. Ye, H. Zhang, J. Fei, C. Wolstenholme, Xin Zhang, 2021, Angew. Chem. Int. Ed on p. 1339-1346

A General Strategy to Enhance Donor-Acceptor Molecules Using Solvent-Excluding Substituents

Conner A. Hoelzel, Hang Hu, Charles H. Wolstenholme, Basel A. Karim, Kyle T. Munson, Kwan Ho Jung, Han Zhang, Yu Liu, Hemant P. Yennawar, John B. Asbury, Xiaosong Li, Xin Zhang, 2020, Angewandte Chemie - International Edition on p. 4785-4792

Visualizing and Modulating Biological Processes Using Self-Labeling Protein Tags

Conner Hoelzel, Xin Zhang, 2020, CHEMBIOCHEM on p. 1935-1946

AggFluor: Fluorogenic toolbox enables direct visualization of the multi-step protein aggregation process in live cells

Xin Zhang, 2020, J. Am. Chem. Soc. on p. 17515–17523

Fluorogenic detection of protein aggregates in live cells using the AggTag method

K.H. Jung, Xin Zhang, 2020, Meth. Enzymol. on p. 1-22

Visualizing and manipulating biological processes using Halo-Tag and SNAP-Tag technologies

C.A. Hoelzel, Xin Zhang, 2020, ChemBioChem on p. 1935-1946

Super-resolution optical lithography with DNA

Shi Ho Kim, Yu Liu, Conner Hoelzel, Xin Zhang, Tae Hee Lee, 2019, Nano Letters on p. 6035-6042

A Fluorogenic AggTag Method Based on Halo- and SNAP-Tags to Simultaneously Detect Aggregation of Two Proteins in Live Cells

Kwan Ho Jung, Sojung F. Kim, Yu Liu, Xin Zhang, 2019, ChemBioChem on p. 1078-1087

A SNAP-tag fluorogenic probe mimicking the chromophore of the red fluorescent protein Kaede

Kwan Ho Jung, Matthew Fares, Leeann S. Grainger, Charles H. Wolstenholme, Anna Hou, Yu Liu, Xin Zhang, 2019, Organic and Biomolecular Chemistry on p. 1906-1915

A HaloTag-based multi-color fluorogenic sensor visualizes and quantifies proteome stress in live cells using solvatochromic and molecular rotor-based fluorophores

Yu Liu, Kun Miao, Yinghao Li, Matthew Fares, Shuyuan Chen, Xin Zhang, 2018, Biochemistry on p. 4663-4674

Most-Cited Papers

Mechanisms of transthyretin inhibition of β-amyloid aggregation in vitro

Xinyi Li, Xin Zhang, Ali Reza A. Ladiwala, Deguo Du, Jay K. Yadav, Peter M. Tessier, Peter E. Wright, Jeffery W. Kelly, Joel N. Buxbaum, 2013, Journal of Neuroscience on p. 19423-19433

Apoferritin-CeO <sub>2</sub> nano-truffle that has excellent artificial redox enzyme activity

Xiangyou Liu, Wei Wei, Quan Yuan, Xin Zhang, Ning Li, Yuguang Du, Guanghui Ma, Chunhua Yan, Ding Ma, 2012, Chemical Communications on p. 3155-3157

Modulation of fluorescent protein chromophores to detect protein aggregation with turn-on fluorescence

Yu Liu, Charles H. Wolstenholme, Gregory C. Carter, Hongbin Liu, Hang Hu, Leeann S. Grainger, Kun Miao, Matthew Fares, Conner A. Hoelzel, Hemant P. Yennawar, Gang Ning, Manyu Du, Lu Bai, Xiaosong Li, Xin Zhang, 2018, Journal of the American Chemical Society on p. 7381-7384

Molecular dynamics simulation reveals preorganization of the chloroplast FtsY towards complex formation induced by GTP binding

Ming Jun Yang, Xue Qin Pang, Xin Zhang, Ke Li Han, 2011, Journal of Structural Biology on p. 57-66

Fidelity of cotranslational protein targeting by the signal recognition particle

Xin Zhang, Shu Ou Shan, 2014, Annual Review of Biophysics on p. 381-408

Carbon nitride nanothread crystals derived from pyridine

Xiang Li, Tao Wang, Pu Duan, Maria Baldini, Haw Tyng Huang, Bo Chen, Stephen J. Juhl, Daniel Koeplinger, Vincent Henry Crespi, Klaus Schmidt-Rohr, Roald Hoffmann, Nasim Alem, Malcolm Guthrie, Xin Zhang, John V. Badding, 2018, Journal of the American Chemical Society on p. 4969-4972

The cation-Pi interaction enables a halo-tag fluorogenic probe for fast no-wash live cell imaging and gel-free protein quantification

Yu Liu, Kun Miao, Noah P. Dunham, Hongbin Liu, Matthew Fares, Amie K. Boal, Xiaosong Li, Xin Zhang, 2017, Biochemistry on p. 1585-1595

AgHalo: A facile fluorogenic sensor to detect drug induced proteome stress

Yu Liu, Matthew Fares, Noah P. Dunham, Zi Gao, Kun Miao, Xueyuan Jiang, Samuel S. Bollinger, Amie Kathleen Boal, Xin Zhang, 2017, Angewandte Chemie - International Edition on p. 8672-8676

Fluorescence Turn-On Folding Sensor to Monitor Proteome Stress in Live Cells

Yu Liu, Xin Zhang, Wentao Chen, Yun Lei Tan, Jeffery W. Kelly, 2015, Journal of the American Chemical Society on p. 11303-11311

Individual and collective contributions of chaperoning and degradation to protein homeostasis in E. coli

Younhee Cho, Xin Zhang, Kristine Faye R. Pobre, Yu Liu, David L. Powers, Jeffery W. Kelly, Lila M. Gierasch, Evan T. Powers, 2015, Cell Reports on p. 321-333