Workshop Day 2: Structural and Biophysical Methods in Biomolecular Research

X-Ray Crystallography, Scattering and Biomolecular Interactions Core Facilities

Day 2 Schedule

Zoom:

9:00 a.m. - Biophysical and biochemical studies of Farnesoid X receptor homodimer; Sabab Khan, assistant research professor, Okafor Lab, Dept. of Biochem & Mol. Biology

9:15 a.m. - Structural characterization of Fabl-PFAS interactions; Avanti Vairagkar, grad student, Patterson Lab, Dept. of Vet & Biomed Science

9:30 a.m. - Applying isothermal calorimetery, differential, scanning calorimetry and circular dichroism to, study intermolecular interactions, thermodynamic properties and structure; Neela Yennawar, director, Biomolecular Interactions & X-ray Crystallography & Scattering Cores, Huck Institutes

10:15 a.m. - Introduction to Cryo-EM: From sample, preparation and biophysical characterization to grid preparation, data collection and processing; Ibrahim Moustafa, asst research professor, Biomolecular Interactions & X-ray Crystallography & Scattering Cores, Dept. of Biochem & Mol. Biology

11:00 a.m. - Protein complex characterization by size-exclusion chromatography coupled with dynamic and multi-angle light scattering; Aubree O'Neill, light scattering specialist, Waters-Wyatt Technologies, Goleta, CA

8 Althouse Lab:

1:30 p.m. - Step-by-step Cryo-EM data processing with cryoSPARC; Ibrahim Moustafa, asst research professor, Biomolecular Interactions & X-ray Crystallography & Scattering Cores, Dept. of Biochem & Mol. Biology

3:00 p.m. - Isothermal titration calorimetry and differential scanning calorimetry study of copper sulfate-bovine serum albumin binding, and circular dichroism analysis of lysozyme; Julia Fecko, research technologist, Biomolecular Interactions & X-ray Crystallography & Scattering Cores, Huck Institutes

Contact

  Neela Yennawar
  nhy1@psu.edu