X-Ray Crystallography Facility

Fortebio Octet QK

The first generation Octet to perform label-free quantitation of antibodies, proteins, peptides, DNA and other biomolecules and provide kinetic characterization of biomolecular binding interactions

Uses

  • Epitope mapping/binding
  • Yes/No binding
  • Automated IgG concentration determinations
  • Custom protein concentration determinations

Details

Detection technology

Bio-layer interferometry (BLI)

Capabilities

  • Kinetic and affinity data: kobs, ka, kd, KD
  • Binding specificity and cooperativity

Sampling format

Test plate
  • 96-well, black, flat bottom, polypropylene microplate
Test volume
  • 100-200 µL
  • Application-dependent
  • Non-destructive and recoverable

Sample types

  • Proteins
  • Antibodies
  • Peptides

Buffer compatibility

  • Cell media
  • Crude lysates
  • Untreated sups
  • Periplasmic fractions
  • Serum containing media (up to 10%)
  • DMSO

Analysis runtime per interaction

  • Variable, up to 3 hours total

Typical working ranges

Kinetics
  • Association rate constant: (ka) 101 to 105 M-1 s-1
  • Dissociation rate constant: (kd) 10-6 to 10-3 s-1
  • Affinity range: 10-4 to 10-10 M
Quantitation
  • 1 µg/mL to 500 µg/mL (protein and assay–dependent)
Molecular weight detection
  • >10 kDa

Automation

  • Up to 8 biosensors in parallel
  • Maximum of 96 tests unattended

Test plate temperature

  • 2°C over ambient to 40°C
  • 1°C increments

Orbital flow

  • Static or 100-1500 rpm

Data handling and storage

  • Integrated software package for operation and data analysis
  • Microsoft® Windows® XP Professional
  • RS232 and USB interfacing