Transgenic Mouse Production

Production of transgenic mice by pronuclear injection

What you will need:

  • An approved Penn State Institutional Animal Care and Use Committee protocol so that any transgenic progeny can be transferred to your protocols at weaning
  • Evidence of a screening assay, either PCR or Southern, for your proposed transgene
  • Consulted with the Animal Resources Program to acquire mouse space in one of the Penn State campus facilities
  • Consulted with us to establish protocols for isolation of and preparation of DNA constructs