Production of transgenic mice by pronuclear injection
What you will need:
- An approved Penn State Institutional Animal Care and Use Committee protocol so that any transgenic progeny can be transferred to your protocols at weaning
- Evidence of a screening assay, either PCR or Southern, for your proposed transgene
- Consulted with the Animal Resources Program to acquire mouse space in one of the Penn State campus facilities
- Consulted with us to establish protocols for isolation of and preparation of DNA constructs