Gene-Targeted Mouse Production

Production of targeted mutant mice by embryonic stem cell microinjection

What you will need:

  • An approved Penn State Institutional Animal Care and Use Committee protocol so that chimeric progeny can be transferred to your protocols at weaning
  • Consultation with the Animal Resources Program to acquire mouse space in one of the Penn State campus facilities
  • Evidence of a screening assay (either PCR or Southern), for your mutant allele, so you can detect germline transmission
  • Investigators who want to supply their own targeted embryonic stem cell line must have commercial documentation that the cell line was MAP tested for murine adventitious agents prior to expansion