Research Publications

Facility offers highly sensitive and accurate mass spectrometry analysis, including identification and quantitation of proteins by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) or by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-TOF-MS).

RRID

SCR_024462

Research Publications Supported by This Facility

• Degradation factor 1, Def1, regulates mRNA translation and decay through Ccr4-Not-dependent ubiquitylation of the ribosome.
  Akinniyi OT, Sebastian A, Kulkarni S, Albert I, Reese JC
  RNA. 2026 May 18; 32(6): 930-944. doi: 10.1261/rna.080884.125. 2026 May 18.
• Enabling Fluoroalkyl-Sulfonylalkylation and Fluoroalkyl-Halogenation of Alkenes and Alkynes via Photoredox Catalysis.
  Hewa Inaththappulige SIN, Acharya A, Agampodi NDDZ, Singh H, Giri R
  J Am Chem Soc. 2026 Apr 29; 148(16): 16882-16893. doi: 10.1021/jacs.6c00567. 2026 Apr 17.
• FLEXTAG: a small and self-renewable protein labeling system for anti-fading multi-color super-resolution imaging.
  Zhang H, Yao Y, Wang X, Zheng Y, Zhang S, Tao Y, Yang H, Wang Y, Liu M, Feric M, Zhou R
  Nat Commun. 2026 Mar 9; 17(1): . doi: 10.1038/s41467-026-69658-9. 2026 Mar 9.
• Increasing the Compositional Heterogeneity of Single-Chain Amphiphile Membranes Supported by Coacervate Cores Alters Stability and Properties of the Hybrid Protocells.
  Joshi MP, Lee J, Chan M, Keating CD
  Langmuir. 2026 May 19; 42(19): 13582-13595. doi: 10.1021/acs.langmuir.6c00637. 2026 May 1.
• Oxidative stress impairs processive motility of the axonal transport motor KIF1A.
  Chen AP, Pandey H, Hancock WO
  J Biol Chem. 2026 Jun; 302(6): 111471. doi: 10.1016/j.jbc.2026.111471. 2026 Apr 17.
• PAX3-FOXO1 Contacts BRD4 through Its Acetylated Intrinsically Disordered Region.
  Fraser OA, Schleicher MN, Pagano ML, Showalter SA
  Biochemistry. 2026 Jul 7; 65(13): 2066-2076. doi: 10.1021/acs.biochem.6c00040. 2026 Jun 12.
• Gene expression analysis and proximity labeling reveal post-transcriptional functions of the yeast RNA polymerase II regulator Def1.
  Akinniyi OT, Kulkarni S, Hribal MM, Keller CA, Giardine BM, Reese JC
  J Biol Chem. 2026 Feb; 302(2): 111003. doi: 10.1016/j.jbc.2025.111003. 2025 Dec 5.
• Proteomic insights into Pholiota nameko mycelial response to wood-based substrates.
  Kothattil S, Pecchia JA, O'Connor E
  Fungal Biol. 2026 Feb; 130(1): 101714. doi: 10.1016/j.funbio.2025.101714. 2025 Dec 19.
• Kinetically Divergent Dimanganese-Tyrosyl Radical Cofactor Assembly in Listeria monocytogenes Class I Ribonucleotide Reductase.
  Chong PG, Biswas S, Chen C, Cotruvo JA Jr
  Biochemistry. 2026 Aug 4; 65(15): 2387-2399. doi: 10.1021/acs.biochem.6c00369. .
• FLEXTAG: A Small and Self-renewable Protein Labeling System for Anti-fading Multicolor Super-resolution Imaging.
  Zhang H, Yao Y, Wang X, Tao Y, Yang H, Wang Y, Liu M, Feric M, Zhou R
  bioRxiv. 2025 Jul 23; (): . pii: 2025.07.19.665678. doi: 10.1101/2025.07.19.665678. 2025 Jul 23.
• Auxin-induced depletion of human CCR4-NOT subunits reveals opposing functions of CNOT1 and CNOT4 in mRNA metabolism.
  Kulkarni S, Smith C, Akinniyi OT, Giardine BM, Keller CA, Arnaoutov A, Reese JC
  J Biol Chem. 2025 Dec; 301(12): 110862. doi: 10.1016/j.jbc.2025.110862. 2025 Oct 27.
• Degradation factor 1, Def1, regulates mRNA translation and decay through Ccr4-Not-dependent ubiquitylation of the ribosome.
  Akinniyi OT, Sebastian A, Kulkarni S, Albert I, Reese JC
  bioRxiv. 2025 Dec 12; (): . pii: 2025.12.10.693450. doi: 10.64898/2025.12.10.693450. 2025 Dec 12.
• Anti-miR oligo-mediated detection of human salivary microRNAs for mild traumatic brain injury.
  Saha P, van der Vlies AJ, Aditya T, Dighe K, Hicks SD, Pan D
  Biosens Bioelectron. 2025 Jun 15; 278(): 117333. doi: 10.1016/j.bios.2025.117333. 2025 Mar 2.
• Identifying bacterial heme sensor protein interacting partners under varying oxygen tensions using proximity labeling.
  Fekete FJ, Weinert EE
  J Inorg Biochem. 2026 Jan; 274(): 113067. doi: 10.1016/j.jinorgbio.2025.113067. 2025 Sep 9.
• Probing Enzymatic Acetylation Events in Real Time With NMR Spectroscopy: Insights Into Acyl-Cofactor Dependent p300 Modification of Histone H4.
  Dewing SM, Showalter SA
  Proteins. 2025 Oct; 93(10): 1837-1847. doi: 10.1002/prot.26848. 2025 Jun 1.
• The DNA damage response and RNA Polymerase II regulator Def1 has posttranscriptional functions in the cytoplasm.
  Akinniyi OT, Sebastian A, Kulkarni S, Hribal MM, Keller CA, Giardine BM, Albert I, Reese JC
  bioRxiv. 2025 Apr 8; (): . pii: 2024.09.16.613278. doi: 10.1101/2024.09.16.613278. 2025 Apr 8.
• Hydrophobicity analysis of Chinese Hamster Ovary host cell proteins using analytical hydrophobic interaction chromatography and LC-MS.
  Chu LK, Laremore T, Borujeni EE, Xu X, Zydney AL
  J Chromatogr A. 2025 Nov 22; 1763(): 466440. doi: 10.1016/j.chroma.2025.466440. 2025 Oct 4.
• Acetylation-Dependent Compaction of the Histone H4 Tail Ensemble.
  Dewing SM, Phan TM, Kraft EJ, Mittal J, Showalter SA
  J Phys Chem B. 2024 Oct 31; 128(43): 10636-10649. doi: 10.1021/acs.jpcb.4c05701. 2024 Oct 22.
• Human CCR4-NOT globally regulates gene expression and is a novel silencer of retrotransposon activation.
  Kulkarni S, Morrissey A, Sebastian A, Giardine B, Smith C, Akinniyi OT, Keller CA, Arnaoutov A, Albert I, Mahony S, Reese JC
  bioRxiv. 2024 Nov 25; (): . pii: 2024.09.10.612038. doi: 10.1101/2024.09.10.612038. 2024 Nov 25.