Zeiss LSM 880

The Zeiss LSM 880 Airyscan Fast with PicoQuant FLIM is a high-end confocal microscope.

Microscopy Facility

433 Wartik Lab
360 Science Dr
University Park, PA 16802

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Specifications

  • Resolution: The Airyscan and Airyscan Fast model allows for super resolution imaging to resolve structures of 130 nm (in x, y) and 350 nm (in z) even in thick samples.
  • Speed: The Airyscan Fast module allows for super resolution imaging at a fast mode with acquisition speeds of upto 27fps (at 480 x 480 pixels).
  • Sensitivity: The microscope’s 34 channel NLO array detectors can obtain sharp images at a very low (0.01 %) laser powers and thus is suited to imaging prolonged live-cell imaging, reducing the likelihood of photobleaching and phototoxicity. This feature also makes easier to perform quantitative microscopy, such as FRAP, FRET, FLIM, FCS and other F-functionalities.
  • Seven laser lines (405, 458, 488, 514, 561, 594, 633 nm): provides a broad range of fluorophores to be exited for imaging.
  • Objective lens: 5x, 10x, 20x, 40x/1.2w, 40x/1.3, 63x/1.4, 100x/1.46
  • Incubator for long-term Life-cell imaging
  • CherryTemp Core Platform for rapid temperature shift experiments
  • Definite Focus and Z-piezo ensure auto-focusing and precise stage movement.
  • PicoQuant-FLIM LSM upgrade KIT with four PLs (440, 485, 530, 561 nm). This kit allows for fluorescence-lifetime imaging microscopy to add fluorescence lifetime as an additional domain in imaging and quantitative microscopy analysis.

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Microscopy Core Facility (RRID: SCR_024457) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.