Uses
- Cell sorting [multi-color, fluorescent protein etc.] for culture or sequencing
- Nuclei sorting for genomics
- Rare population enrichment
- Single cell clone /cell line derivation
- Plate sorting [6 well, 12 well, 24 well, 48 well, 96 well] and Index Sorting
Specifications
- Five spatially separated lasers (UV 349nm, Violet 405nm, Blue 488nm, YG 561nm, and Red 640nm)
- 53 parameters: 48 fluorescent parameter detectors and 5 light scatter detectors
- 405nm laser based FSC- SPD (small particle detector)
- Spectral unmixing and conventional compensation
- Nozzles (70µm, 100µm, 120µm, and 150µm) with adjustable pressure settings
- Up to 6-way sorting
- Temperature control from 4°C to 37°C
- Integrated biocontainment enclosure (Class II) and aerosol management system
Acknowledgement
Users of this instrument are required to acknowledge the Huck Institutes’ Flow Cytometry Core Facility (RRID: SCR_024460) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.