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Illumina MiSeq

The Illumina MiSeq is our smallest scale short read sequencer. The sequencing chemistry is similar across all Illumina platforms. Libraries compatible with one Illumina sequencer should be compatible with all Illumina sequencers. The MiSeq produces a modest number of reads. Therefore, it is best used for pilot projects or for applications such as de novo sequencing or resequencing of bacterial and viral genomes, amplicon sequencing, and miRNA/small RNA sequencing.

Genomics Facility

407 Chandlee Lab
209 Pollock Rd
University Park, PA 16802

Phone: 814-867-4067

Illumina MiSeq

Specifications

  • 50 nt single read, 150 x 150 nt paired-end, and 250 x 250 nt paired-end sequencing are available in the standard v2 format.
  • Standard v2 reagent kits produce 15 million single reads (or read pairs).
  • 150 nt single read, 75 x 75 paired-end, and 300 x 300 paired-end sequencing are available in the v3 format.
  • v3 reagent kits produce 24 million single reads (or read pairs).
  • “Nano” reagent kits produce about 1 million single reads (or read pairs) and are available for 150 x 150 nt paired-end, and 250 x 250 nt paired-end sequencing.
  • Sample multiplexing is available for 96 – 384 samples depending on the application.

Details

Data is produced in the FASTQ format.

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Genomics Core Facility (RRID: SCR_023645) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.