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Hydra Bio pFIB-SEM

The Hydra Bio pFIB-SEM is a versatile system that supports both room-temperature and cryogenic workflows. It prepares lamella for volume EM and cryo-ET, performs slice-by-slice SEM imaging, and integrates iFLM for correlative light and electron microscopy (CLEM). This system is equipped with multiple chemical protection sources to mitigate charging and reduce milling artifacts.

Cryo-Electron Microscopy Facility

Millennium Science Complex, N034, N030F, N030H
491 Pollock Rd
University Park, PA 16802

Uses

  • Lamella preparation for CryoET and Volume EM
  • Correlative light electron microscopy (CLEM)
  • Slice-by-slice SEM imaging

Specifications

  • Four different ion sources (xenon, argon, nitrogen, and oxygen) for fast milling
  • The integrated cryo-stage and cryo-transfer module provide a stable cryogenic sample preparation environment
  • The Easylift Cryo Lift-Out System supports lamella preparation from bulk high-pressure frozen (HPF) specimens
  • Integrated fluorescence light microscope (iFLM) correlates the light microscopy information with the SEM data
  • Three chemical coating materials (Platinum, Carbon, and Tungsten) for a wider range of protection
  • AutoTEM Cryo, MAPS 3, and Auto Slice and View software packages facilitate sample preparation and data collection

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Cryo-Electron Microscopy Core Facility RRID (SCR_024456) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.