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FEI Titan Krios

The FEI Titan Krios is an instrument that realizes the revolutionary developments in cryo-EM leading to the “resolution revolution” that allows atomic resolution maps of biological objects with low or no symmetry. These transformative advances include the exceptionally stable microscope stage, automated data collection, direct electron detection technology, fast read out for data transfer. However, our Penn State Krios microscope is one of a kind.

Cryo-Electron Microscopy Facility

Millennium Science Complex, N034, N030F, N030H
491 Pollock Rd
University Park, PA 16802

Uses

  • Screening of proteins, viruses, nanoparticles, etc. at cryogenic temperature
  • Data collection of single particles for 3D reconstruction
  • Data collection for tomography

Specifications

  • Cs (spherical aberration) corrector​
  • Falcon 4i direct electron detector
  • Selectris X energy filter
  • Fringe-Free Imaging (FFI)
  • Cryo-Flow for real-time quality check during data collection
  • EPU software
  • Tomography software
  • CryoSparc-live
  • The best resolution (1.46A) from apoferritin

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Cryo-Electron Microscopy Core Facility RRID (SCR_024456) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.