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​BioVision VT-iSIM

With no requirement to use specific fluorophores and without any computation, this system produces super-resolution images in real time at up to 1,000 frames per second. This high temporal resolution is combined with a spatial resolution enhancement on all axes of up to 2× that of conventional fluorescence microscopes.

Microscopy Facility

433 Wartik Lab
360 Science Dr
University Park, PA 16802

Penn State shield logo featuring a stylized mountain lion in white and light blue on a blue gradient with white s-curve background.

Specifications

  • Resolution: 125 nm laterally and 350 nm axially
  • Scan speed up to 1000 fps, full frame
  • Six SS lasers from visible range (405, 445, 488, 514, 561, and 642)
  • Hamamatsh ORCA-Flash 4.0 VT+ sCMOS
  • Objective lens
  • Nikon CFI Apochromat TIRF 100× oil (NA1.49)
  • Nikon CFI Apochromat TIRF 60× oil (NA1.45)
  • Nikon CFI PlanApochromat Lambda 20x (NA0.75)
  • Software: MetaMorph

Details

For more information visit http://www.biovis.com/vtisim.html

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Microscopy Core Facility (RRID: SCR_024457) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.