5L Cytek Aurora – Spectral Analyzer

The Cytek Aurora is a full spectrum flow cytometer which enables extraction of autofluorescence and resolves highly overlapping signals using spectral unmixing.

Flow Cytometry Facility

W124A Millennium Science Complex
491 Pollock Rd
University Park, PA 16802

Phone: 814-865-6595

5L Cytek Aurora – Spectral Analyzer

Uses

  • Immunophenotyping (30+ color analysis)
  • Cell Cycle / DNA Ploidy Analysis
  • Apoptosis Assays; Proliferation Assays
  • Stem Cell Analysis; Rare Event Analysis
  • Fluorescent Protein Analysis (BFP, GFP, mCherry, dtomato etc.)
  • Functional Assays (Lysis, Mitochondrial Potential etc.)
  • Cell Line Characterization
  • Cell-based Therapeutics and Biomarker Evaluation

Specifications

  • Five spatially separated lasers (UV 355nm, Violet 405nm, Blue 488nm, YG 561nm, and Red 640nm)
  • 64 fluorescent parameter detectors (16UV-16V-14B-10YG-8R) and 3 light scatter detectors
  • 405nm violet laser-based SSC
  • Spectral unmixing [SpectroFlo® software]
  • Autofluorescence extraction and Full Spectrum Profiling (FSP) of cells
  • Capable of resolving 0.1 μm beads from noise. ESP Detection
  • Automated sample loader for 96-well plates and 40-tube racks

Acknowledgement

Users of this instrument are required to acknowledge the Huck Institutes’ Flow Cytometry Core Facility (RRID: SCR_024460) in any resulting publications. Please review the acknowledgment and co-authorship guidelines for full details.