Illumina NextSeq

Illumina NextSeq
Illumina NextSeq

The Illumina NextSeq is our newest short-read sequencer, and replaces the obsolete HiSeq 2500.   


  • Single flow cell instrument. One library pool per flow cell, no individual lane loading.
  • High-Output flow cells produce 400 million reads (or 400 million pairs of reads) per run.
  • 75 nt single read, 75 x 75 nt paired-end read, 150 nt single read, or 150 x 150 paired-end read sequencing available from High-Ouput flow cells.
  • Mid-Output flow cells produce 120 million reads (or 120 million pairs of reads) per run.
  • 75 x 75 paired-end sequencing, 150 nt single read sequencing, 150 x 150 paired-end sequencing available from Mid-Output flow cells.
  • Sample multiplexing available up to 96-384, depending on the application.


The sequencing chemistry is similar across all Illumina platforms. Libraries compatible with one Illumina sequencer should be compatible with all Illumina sequencers.

Used for a variety of applications, including but not limited to:

  • De novo whole genome sequencing
  • Whole genome resequencing
  • Exome sequencing
  • mRNA-Seq
  • miRNA/small RNA-Seq
  • ChIP-Seq
  • RIP-Seq
  • Degradome sequencing

Data produced in the FASTQ format.

This instrument is operated by Genomics Core Facility staff in conjunction with next-generation sequencing services